Novel molecular mechanism maintaining genomic homeostasis against environmental stresses
Project/Area Number |
24241019
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Risk sciences of radiation/Chemicals
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Research Institution | Kobe University |
Principal Investigator |
SUGASAWA Kaoru 神戸大学, 自然科学系先端融合研究環バイオシグナル研究センター, 教授 (70202124)
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Co-Investigator(Kenkyū-buntansha) |
IWAI Shigenori 大阪大学, 基礎工学研究科, 教授 (10168544)
|
Co-Investigator(Renkei-kenkyūsha) |
YOKOTA Hiroaki 光産業創成大学院大学, 光産業創成研究科, 准教授 (90415547)
YOSHINO Ken-ichi 神戸大学, 自然科学系先端融合研究環バイオシグナル研究センター, 助教 (90280792)
|
Research Collaborator |
AKITA Masaki
MATSUMOTO Syota
TONE Daisuke
ONISHI Yuki
KINOSHITA Minoru
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥43,030,000 (Direct Cost: ¥33,100,000、Indirect Cost: ¥9,930,000)
Fiscal Year 2014: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2013: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2012: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
|
Keywords | 遺伝子 / ゲノム / 蛋白質 / DNA損傷修復 / 一分子解析 |
Outline of Final Research Achievements |
This research project was aimed to understand the molecular bases, which enable the global genomic nucleotide excision repair (GG-NER) pathway to efficiently survey genomic DNA for damage caused by various environmental stresses. By using the defined cell-free GG-NER system reconstituted with purified recombinant protein factors, it was shown that certain endogenous DNA lesions, such as abasic sites, facilitate the damage recognition process in GG-NER, and that DNA repair efficiency is affected by the topological state of damaged DNA as well as protein phosphorylation. We also elucidated novel roles of ubiquitination and SUMOylation of the xeroderma pigmentosum group C (XPC) protein in recognition of ultraviolet (UV)-induced DNA photolesions mediated by the UV-damaged DNA binding protein complex. Finally, single molecule imaging experiments revealed one-dimensional diffusion of the XPC protein during the search for lesions along DNA.
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] Functional regulation of the DNA damage-recognition factor DDB2 by ubiquitination and interaction with xeroderma pigmentosum group C protein.2015
Author(s)
Syota Matsumoto, Eric S. Fischer, Takeshi Yasuda, Naoshi Dohmae, Shigenori Iwai, Toshio Mori, Ryotaro Nishi, Ken-ichi Yoshino, Wataru Sakai, Fumio Hanaoka, Nicolas H. Thoma, Kaoru Sugasawa
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Journal Title
Nucleic Acids Research
Volume: 3
Issue: 3
Pages: 1700-1713
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Fluorescence detection of cellular nucleotide excision repair of damaged DNA.2014
Author(s)
Toga, T., Kuraoka, I., Watanabe, S., Nakano, E., Takeuchi, S., Nishigori, C., Sugasawa, K. and Iwai, S.
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Journal Title
Scientific Reports
Volume: 4
Issue: 1
Pages: 5578-5578
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] PRDM14 promotes active DNA demethylation through the Ten-eleven translocation (TET)-mediated base excision repair pathway in embryonic stem cells.2014
Author(s)
Okashita, N., Kumaki, Y., Ebi, K., Nishi, M., Okamoto, Y., Nakayama, M., Hashimoto, S., Nakamura, T., Sugasawa, K., Kojima, N., Takada, T., Okano, M. and Seki, Y.
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Journal Title
Development
Volume: 141
Issue: 2
Pages: 269-280
DOI
Related Report
Peer Reviewed
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[Journal Article] Comparative analysis of interaction of human and yeast DNA damage recognition complexes with damaged DNA in nucleotide excision repair.2013
Author(s)
Krasikova, Y. S., Rechkunova, N. I., Maltseva, E. A., Pestryakov, P. E., Petruseva, I. O., Sugasawa, K., Chen, X., Min, J. H. and Lavrik, O. I.
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Journal Title
Journal of Biological Chemistry
Volume: 288
Issue: 15
Pages: 10936-10947
DOI
Related Report
Peer Reviewed
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[Journal Article] Structure-specific endonucleases Xpf and Mus81 play overlapping but essential roles in DNA repair by homologous recombination.2013
Author(s)
Kikuchi K, Narita T, Pham 〓, Iij〓 J, Hirota K, Islam SK, Mohiuddin M, Okawa K, Bori T, Fukagama T, Essers J, Kanser R, Whithy NC, Sugasawa K, Taniguchi T, Kitagawa K. Takeda S.
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Journal Title
Cancer Res.
Volume: (in press)
Issue: 14
Pages: 6917-6929
DOI
Related Report
Peer Reviewed
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[Journal Article] Human and yeast DNA damage recognition complexes bind with high affinity DNA structures mimicking in size transcription bubble.2013
Author(s)
Krasikova, Y. S., Rechkunova, N. I., Maltseva, E. A., Anarbaev, R. O., Pestryakov, P. E., Sugasawa, K., Min, J. H. and Lavrik, O. I.
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Journal Title
Journal of Molecular Recognition
Volume: 26
Issue: 12
Pages: 653-661
DOI
Related Report
Peer Reviewed
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