• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Cooperative Conformational Dynamics of Ring-Shaped ATPase studied by High-Speed AFM/Fluorescence Microscopy

Research Project

Project/Area Number 24241048
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Nanomaterials/Nanobioscience
Research InstitutionKanazawa University

Principal Investigator

UCHIHASHI TAKAYUKI  金沢大学, 数物科学系, 教授 (30326300)

Co-Investigator(Kenkyū-buntansha) IINO Ryota  大学共同利用機関法人自然科学研究機構, 岡崎統合バイオサイエンスセンター, 教授 (70403003)
WATANABE Yo-hei  甲南大学, 理工学部, 准教授 (40411839)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥46,930,000 (Direct Cost: ¥36,100,000、Indirect Cost: ¥10,830,000)
Fiscal Year 2014: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2013: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Fiscal Year 2012: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Keywords一分子イメージング / 走査型プローブ顕微鏡 / 高速原子間力顕微鏡 / タンパク質 / 分子シャペロン / 構造変化 / 一分子計測 / 蛋白質 / ATPase / 走査プローブ顕微鏡 / AAAタンパク質 / シャペロニン / 蛍光顕微鏡
Outline of Final Research Achievements

We observed conformational dynamics of a molecular chaperone ClpB which disaggregates denatured proteins. We found that the hexameric ring of the TClpB dynamically changes between symmetric, asymmetric and broken ring. Also it was indicated that the dynamic fluctuation of the ring structure is essential for the chaperon activity of ClpB.
We also improved combined system high-speed AFM and fluorescent microscopy. The combined system enabled simultaneous single-molecule imaging with high-speed AFM and fluorescence microscopy. We succeeded in observing processive movement of enzymatic chitinase with fluorophores along a chitin fibril and further conformational change of F1-ATPase and binding/dissociation of fluorescent-ATP analogue. We have applied high-speed AFM to investigate cooperative conformational change of V1-ATPase without the shaft (DF subunit). We found that conformational change of the A subunit propagates unidirectionally in the hexameric ring even without the shaft.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (59 results)

All 2015 2014 2013 2012 Other

All Journal Article (22 results) (of which Peer Reviewed: 18 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (32 results) (of which Invited: 26 results) Book (5 results)

  • [Journal Article] Long-tip high-speed atomic force microscopy for nanometer-scale imaging in live cells2015

    • Author(s)
      M. Shibata, T. Uchihashi, T. Ando and R. Yasuda
    • Journal Title

      Scientific Reports

      Volume: 5 Pages: 8724-8724

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Probing structural dynamics of an artificial protein cage using high-speedatomic force microscopy2015

    • Author(s)
      M. Imamura, T. Uchihashi, T. Ando, A. Leifert, U. Simon, A. D. Malay and J. G. Heddle
    • Journal Title

      Nano Letters

      Volume: 15 Issue: 2 Pages: 1331-1335

    • DOI

      10.1021/nl5045617

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two-way traffic of glycoside hydrolase family 18 processive chitinases on crystalline chitin2014

    • Author(s)
      K. Igarashi, T. Uchihashi, T. Uchiyama, H. Sugimoto, M. Wada, K. Suzuki, S. Sakuda, T. Ando, T. Watanabe, and M. Samejima
    • Journal Title

      Nature Communications

      Volume: 5 Pages: 2975-2975

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] キチナーゼによる結晶性キチンのプロセッシブ(連続的)な分解機構の解明2014

    • Author(s)
      杉本華幸, 五十嵐圭日子, 内橋貴之, 鈴木一史, 渡邉剛志
    • Journal Title

      日本応用糖質化学会誌

      Volume: 4 Pages: 107-112

    • Related Report
      2014 Annual Research Report
  • [Journal Article] 高速原子間力顕微鏡による生体試料のダイナミクス観察2014

    • Author(s)
      内橋貴之
    • Journal Title

      膜誌

      Volume: 39 Pages: 322-328

    • NAID

      130005069445

    • Related Report
      2014 Annual Research Report
  • [Journal Article] 高速原子間力顕微鏡による生体分子のナノ動体撮影2014

    • Author(s)
      古寺哲幸, 内橋貴之, 安藤敏夫
    • Journal Title

      日本物理学会誌

      Volume: 69 Pages: 459-464

    • Related Report
      2014 Annual Research Report
  • [Journal Article] ingle-molecule imaging analysis of elementary reaction steps of Trichoderma Reesei cellobiohydrolase I (Cel7A) hydrolyzing crystalline cellulose Iα and IIII2014

    • Author(s)
      Y.Shibafuji, A. Nakamura, T. Uchihashi, N. Sugimoto, S. Fukuda, H. Watanabe, M. Samejima, T. Ando, H. Noji, A. Koivula, K. Igarashi, R. Iino
    • Journal Title

      Journal of Biological Chemistry

      Volume: 印刷中

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Trade-off between Processivity and Hydrolytic Velocity of Cellobiohydrolases at the Surface of Crystalline Cellulose2014

    • Author(s)
      Akihiko Nakamura, Watanabe, H., Ishida, T., Uchihashi, T., Wada, M., Ando, T., Igarashi, K. and Samejima, M.
    • Journal Title

      Journal of the American Chemical Society

      Volume: 136 Issue: 12 Pages: 4584-4592

    • DOI

      10.1021/ja4119994

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Filming Biomolecular Processes by High-Speed Atomic Force Microscopy2014

    • Author(s)
      T. Ando, T. Uchihashi, S. Scheuring
    • Journal Title

      Chemical Reviews

      Volume: 114 Issue: 6 Pages: 3120-3188

    • DOI

      10.1021/cr4003837

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Real-Time Visualization of Assembling of a Sphingomyelin-Specific Toxin.2013

    • Author(s)
      N. Yilmaz, T. Yamada, P. Greimel, T. Uchihashi, T. Ando, T. Kobayashi
    • Journal Title

      Biophys. J.

      Volume: 105 Issue: 6 Pages: 1397-1405

    • DOI

      10.1016/j.bpj.2013.07.052

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-speed atomic force microscope combined with single-molecule fluorescence microscope.2013

    • Author(s)
      S. Fukuda, T. Uchihashi, R. Iino, Y. Okazaki, M. Yoshida, K. Igarashi, T. Ando
    • Journal Title

      Rev. Sci. Instrum.

      Volume: 84 Issue: 7 Pages: 73706-73706

    • DOI

      10.1063/1.4813280

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-speed AFM and applications to biomolecular systems2013

    • Author(s)
      T. Ando, T. Uchihashi, and N. Kodera
    • Journal Title

      Annu. Rev. Biophys

      Volume: 42 Issue: 1 Pages: 393-414

    • DOI

      10.1146/annurev-biophys-083012-130324

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Wide-area scanner for high-speed atomic force microscopy.2013

    • Author(s)
      H. Watanabe, T. Uchihashi, T. Kobashi, M. Shibata, J. Nishiyama, R. Yasuda, T. Ando
    • Journal Title

      Rev. Sci. Instrum.

      Volume: 84 Issue: 5 Pages: 53702-53702

    • DOI

      10.1063/1.4803449

    • NAID

      120005304728

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of trimer-trimer interaction of bacteriorhodopsin studied by optical spectroscopy and high-speed atomic force microscopy2013

    • Author(s)
      H. Yamashita, K. Inoue, M. Shibata, T. Uchihashi, J. Sasaki, H. Kandori, and T. Ando
    • Journal Title

      J. Struct. Biol.

      Volume: (accepted) Issue: 1 Pages: 2-11

    • DOI

      10.1016/j.jsb.2013.02.011

    • NAID

      120005253833

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Real Time Single Molecular Imaging of Enzymatic Degradation of Crystalline Cellulose by High-speed Atomic Force Microscopy2013

    • Author(s)
      五十嵐 圭日子, 内橋 貴之, 鮫島 正浩, 安藤 敏夫
    • Journal Title

      Seibutsu Butsuri

      Volume: 53 Issue: 3 Pages: 140-144

    • DOI

      10.2142/biophys.53.140

    • NAID

      10031159065

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of High-speed Atomic Force Microcscopy2013

    • Author(s)
      内橋貴之, 安藤敏夫
    • Journal Title

      Journal of the Japan Society for Precision Engineering

      Volume: 79 Issue: 3 Pages: 218-222

    • DOI

      10.2493/jjspe.79.218

    • NAID

      130003369799

    • ISSN
      0912-0289, 1882-675X
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] リアルタイム原子間力顕微鏡の開発とたんぱく質の機能動態イメ  ージング2013

    • Author(s)
      内橋貴之, 古寺哲幸
    • Journal Title

      光学

      Volume: 42 Pages: 89-94

    • NAID

      10031154878

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] リアルタイム原子間力顕微鏡の開発とたんぱく質の機能動態イメージング2013

    • Author(s)
      内橋貴之
    • Journal Title

      光学

      Volume: 42 Pages: 89-94

    • NAID

      10031154878

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Visualization of cellobiohydrolase I from Trichoderma reesei moving on crystalline cellulose using high-speed atomic force microscopy2012

    • Author(s)
      Kiyohiko Igarashi
    • Journal Title

      Methods in Enzymology

      Volume: 510 Pages: 169-182

    • DOI

      10.1016/b978-0-12-415931-0.00009-4

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Guide to video recording of structure dynamics and dynamic processes of proteins by high-speed atomic force microscopy2012

    • Author(s)
      Takayuki Uchihashi
    • Journal Title

      Nature Protocols

      Volume: 7 Pages: 1193-1206

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single molecule imaging on living bacterial cell surface by high-speed AFM2012

    • Author(s)
      Hayato Yamashita
    • Journal Title

      Journal of Molecular Biology

      Volume: 422 Issue: 2 Pages: 300-309

    • DOI

      10.1016/j.jmb.2012.05.018

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-speed atomic force microscopy2012

    • Author(s)
      T. Ando, T. Uchihashi, and N. Kodera
    • Journal Title

      Jpn. J. Appl. Phys

      Volume: 51 Issue: 8S3 Pages: 08KA02-08KA02

    • DOI

      10.1143/jjap.51.08ka02

    • NAID

      210000141129

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] High-speed atomic force microscope for imaging of biomolecular dynamics at solid surface2015

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      10th Annual International Electromaterials Science Symposium
    • Place of Presentation
      Wollongong, Australia
    • Year and Date
      2015-02-11
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Visualization of single molecule dynamics at work with high-speed atomic force microscopy2015

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      The 7th Biennial Australian Colloid & Interface Symposium
    • Place of Presentation
      Hobart, Tasmania, Australia
    • Year and Date
      2015-02-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 高速原子間力顕微鏡によるタンパク質のダイナミクスと物性計測2015

    • Author(s)
      内橋貴之
    • Organizer
      NIBBバイオイメージングフォーラム「物理特性のイメージング」
    • Place of Presentation
      基礎生物学研究所, 愛知県
    • Year and Date
      2015-01-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 高速原子間力顕微鏡の開発とバイオ応用2015

    • Author(s)
      内橋貴之
    • Organizer
      新世代研究所水和ナノ構造・界面ナノ科学合同研究会「固液界面の水和ナノ構造と生体高分子ダイナミクス」
    • Place of Presentation
      伊豆長岡温泉 天坊, 静岡県
    • Year and Date
      2015-01-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] High-speed atomic force microscope for studying dynamic interactions in biomolecular system2015

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      The 3rd International Symposium on Dynamical Ordering of Biomolecular Systems for Creation of Integrated Functions
    • Place of Presentation
      合歓の郷,三重県
    • Year and Date
      2015-01-10
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Single-molecule imaging of proteins at work with high-speed atomic force microscopy2014

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      16th International Conference on Retinal Proteins
    • Place of Presentation
      長浜ロイヤルホテル, 滋賀県
    • Year and Date
      2014-10-05
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Cooperative Conformational Change in Ring-Shaped ATPase Observed by High-Speed AFM2014

    • Author(s)
      内橋貴之
    • Organizer
      第52回日本生物物理学会年会 Japan-China-Taiwan Joint Symposium on Cooperativity in Supramolecular Machine
    • Place of Presentation
      札幌国際会議場, 北海道
    • Year and Date
      2014-09-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] High-speed atomic force microscopy for imaging of protein dynamics2014

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      Agilent Nanomeasure 2014
    • Place of Presentation
      Beijing, China
    • Year and Date
      2014-09-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 高速AFMによる生体試料のダイナミクス観察2014

    • Author(s)
      内橋貴之
    • Organizer
      日本膜学会第36回年会境界領域シンポジウム
    • Place of Presentation
      早稲田大学, 東京都
    • Year and Date
      2014-05-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Visualization of single molecule dynamics at work with high-speed atomic force microscopy2014

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      Single Protein Dynamics in Cellulo 2014: Spatio-Temporal, Structural and Quantitative Analyses
    • Place of Presentation
      沖縄科学技術大学院大学、沖縄県
    • Year and Date
      2014-04-23
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] High-speed AFM observation of the FliI/ FliJ complex2013

    • Author(s)
      D. Carriou1, T. Uchihashi, Y. Uchida, H. Yanagawa, T. Minamino, K. Imada
    • Organizer
      日本生物物理学会第51回年会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] Nanoscale investigation on bacterial cell surface using high-speed AFM2013

    • Author(s)
      H. Watanabe, C. David, T. Uchihashi, T. Ando
    • Organizer
      日本生物物理学会第51回年会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] Combined system of high-speed-AFM and optical microscopy2013

    • Author(s)
      S. Fukuda1, T. Uchihashi, R. Iino, T. Ando
    • Organizer
      日本生物物理学会第51回年会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] Conformations and dynamics of ClpB hexameric ring observed by high-speed AFM2013

    • Author(s)
      T. Uchihashi, Y. Watanabe, R. Iino, H. Watanabe, T. Yamasaki, T. Ando
    • Organizer
      日本生物物理学会第51回年会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] 高速原子間力顕微鏡によるタンパク質の一分子ダイナミクス計測2013

    • Author(s)
      内橋貴之
    • Organizer
      第90回日本生理学会大会 日本生物物理学会連携シンポジウ ”新しい計測で生命科学を切り拓く”
    • Place of Presentation
      船堀
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] HIGH-SPEED ATOMIC FORCE MICROSCOPY FOR OBSERVING DYNAMIC BIOMOLECULAR PROCESSES2012

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      XXI International Materials Research Congress
    • Place of Presentation
      Cancun, Mexico
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 高速原子間力顕微鏡で可視化するタンパク質の機能動態2012

    • Author(s)
      内橋貴之
    • Organizer
      第52回生命科学夏の学校
    • Place of Presentation
      蒲郡
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] High-speed atomic force microscopy: A tool for nanobionnics2012

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      3rd Asia-Pacific Symposium on Nanobionics
    • Place of Presentation
      Wollongong, Australia
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Image analysis of HS-AFM movies for dynamic events on biological molecules: F1-ATPase and cellulase2012

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      3rd Kanazawa Bio-AFM Workshop
    • Place of Presentation
      Kanazawa
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 高速原子間力顕微鏡でイメージ ングするタンパク質の一分子ダイナミクス2012

    • Author(s)
      内橋貴之
    • Organizer
      第12回名古屋大学遺伝子実験施設公開セミナー
    • Place of Presentation
      名古屋
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 高速AFMによるバイオ分子の液中動的観察2012

    • Author(s)
      内橋貴之
    • Organizer
      生体分子機能解析のための走査型プローブ顕微鏡手法研究部会
    • Place of Presentation
      越後湯沢
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 高速AFMを用いた1分子ダイナミクス2012

    • Author(s)
      内橋貴之
    • Organizer
      第85回日本生化学会大会, シンポジウム”少数性・生化学の新たな視点”
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Combined High-Speed AFM and Optical Microscopy2012

    • Author(s)
      Takayuki Uchihashi
    • Organizer
      20th International Colloquium on Scanning Probe Microscopy (ICSPM20)
    • Place of Presentation
      Okinawa
    • Related Report
      2012 Annual Research Report
  • [Presentation] 高速AFMによる生体分子のダイナミクス解析2012

    • Author(s)
      内橋貴之
    • Organizer
      KEK PF 第1回先進的観測技術研究会
    • Place of Presentation
      つくば
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] HS-AFM observations of conformational dynamics of ring-shaped ATPases: F1 and TClpB

    • Author(s)
      内橋貴之, 飯野亮太, 渡辺洋平, 野地博行, 安藤敏夫
    • Organizer
      231st IMEG seminars & Minisymposium: ATP/GTP駆動分子マシナリーの高速AFMイメージングと分子機構解明の進展
    • Place of Presentation
      熊本大学発生医学研究所
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 高速原子間力顕微鏡による生体試料の動的構造解析 ~一分子から細胞まで~

    • Author(s)
      内橋貴之
    • Organizer
      第1回新学術領域「植物環境感覚」「少数性生物学」ジョイントシンポジウム
    • Place of Presentation
      大阪大学中之島センター
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Application of high-speed atomic force miscroscopy –from proteins to Cells

    • Author(s)
      T. Uchihashi, N. Kodera and T. Ando
    • Organizer
      12th International Conference on Frontiers of Polymers and Advanced Materials
    • Place of Presentation
      Auckland, New Zealand,
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] High-Speed Atomic Force Microscopy for Imaging of Protein Dynamics

    • Author(s)
      T. Uchihashi and T. Ando
    • Organizer
      2013 MRS Fall Meeting, Symposium LL: Advances in Scanning Probe Microscopy
    • Place of Presentation
      Boston, USA
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] High-speed AFM for observing cell dynamics

    • Author(s)
      T. Uchihashi
    • Organizer
      4th Asia-Pacific Symposium on Nanobionics
    • Place of Presentation
      Melbourne, Australia
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 高速原子間力顕微鏡 ~分子の動きを直視して理解する~

    • Author(s)
      内橋貴之
    • Organizer
      生物物理若手の会 第53回夏の学校
    • Place of Presentation
      伊豆長岡温泉
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Single-Molecule Imaging of Proteins at Work with High-Speed Atomic Force Microscopy

    • Author(s)
      T. Uchihashi, N. Kodera and T. Ando
    • Organizer
      IMS Workshop on "Hierarchical Molecular Dynamics: From Ultrafast Spectroscopy to Single Molecule Measurements"
    • Place of Presentation
      岡崎
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 高速AFMによるタンパク質機能動態の直接観察

    • Author(s)
      内橋貴之
    • Organizer
      日本生化学会九州支部例会シンポジウム「タンパク質構造の動きと機能発現」
    • Place of Presentation
      佐賀大学
    • Related Report
      2013 Annual Research Report
  • [Book] “Development of high-speed AFM and its biological applications”, Chapter 8 in Atomic Force Microscopy in Nanobiology2014

    • Author(s)
      T. Uchihahsi, N. Kodera and T. Ando
    • Total Pages
      34
    • Publisher
      Pan Stanford Publishing
    • Related Report
      2014 Annual Research Report
  • [Book] 膜タンパク質構造研究(岩田想編)2013

    • Author(s)
      内橋貴之、安藤敏夫
    • Publisher
      化学同人
    • Related Report
      2013 Annual Research Report
  • [Book] Nanoscale Liquid Interfaces: Wetting, Patterning, and Force Microscopy at the Molecular Scale (edited by Thierry ONDARÇUHU, Jean-Pierre Aimé )2013

    • Author(s)
      T. Ando, T. Uchihashi
    • Publisher
      Pan Stanford Publishing
    • Related Report
      2013 Annual Research Report
  • [Book] Nano-visualization of proteins in action using high-speed AFM" in Single-molecule Studies of Proteins. Biophysics for the Life Sciences Vol. 22013

    • Author(s)
      T. Uchihashi, N. Kodera, T. Ando
    • Publisher
      Springer
    • Related Report
      2013 Annual Research Report
  • [Book] Single-molecule Studies of Proteins. Biophysics for the Life Sciences2013

    • Author(s)
      Takayuki Uchihashi
    • Total Pages
      269
    • Publisher
      Springer
    • Related Report
      2012 Annual Research Report

URL: 

Published: 2013-05-15   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi