|Budget Amount *help
¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2016: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2014: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2012: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
|Outline of Final Research Achievements
The emergence of antibiotic-resistant bacteria has increased the attention to the therapeutic use of bacteriophages. In order to improve the usability of phages, it is required to understand the mechanism underlying host recognition, especially receptor-binding proteins (RBPs) which determine the host range. In this study, we demonstrate staphylococcal phages ΦSA012 possesses at least two RBPs. A polyclonal antibody against ORF103 inhibited infection of ΦSA012 in the presence of α-GlcNAc, suggesting that ORF103 (ligand) binds to α-GlcNAc (Receptor).
Phage-resistant E. coli isolated from co-culture with phage T2 was used. As the target of modification, Hyper Variable Region (HVR) was selected. This region is part of gene38, which encodes the tip of the long tail fiber. CRISPR/Cas system facilitated homologous recombination between wild type phage and plasmid which encoded random mutation in the HVR. Artificially modified phage showed infectivity to the phage resistant E.coli.