|Budget Amount *help
¥45,110,000 (Direct Cost: ¥34,700,000、Indirect Cost: ¥10,410,000)
Fiscal Year 2015: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2014: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2013: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
|Outline of Final Research Achievements
There are many divergent features even in a structurally homologous proteins. It is not clear how they realize these divergent functions on their same structural backbone. To evaluate these molecular divergent mechanism based on common structure, different PYP proteins have been investigated about their molecular mechanism for their features and functions. From the neutron-X-ray combined crystal analysis and very fast time resolved X-ray crystal analysis, there is a unique low barrier hydrogen bond (LBHB) system as a basic component for PYP features. In this basic system there is a special arginine residue which is deprotonated even in a neutral pH condition. Divergent features on PYP molecules were realized by several residues close or distal site from the LBHB. These residues are located on surface region on PYP molecules.