|Budget Amount *help
¥45,370,000 (Direct Cost: ¥34,900,000、Indirect Cost: ¥10,470,000)
Fiscal Year 2015: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2014: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2013: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2012: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
|Outline of Final Research Achievements
We studied the molecular functions of previously identified proteins to elucidate the conserved mechanism of homologous recombination in fission yeast, a eukaryotic model organism. This led to several substantial accomplishments, with the following two discoveries having the highest importance. 1) We determined the crystal structures of the Swi5-Sfr1 complex. Based on the structure, together with biochemical analysis of the structure-function relationship, we have proposed a molecular model for the activation of Rad51-dependent DNA strand exchange. 2) We showed that the helicase and ubiquitin E3 ligase activities of Fbh1 plays a critical role in dual regulation of Rad51-dependent DNA strand exchange. We propose that Fbh1 contributes to the quality control aspect of homologous recombination (DNA repair) in mitosis.