| Budget Amount *help |
¥44,200,000 (Direct Cost: ¥34,000,000、Indirect Cost: ¥10,200,000)
Fiscal Year 2014: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2013: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2012: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
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| Outline of Final Research Achievements |
In this study, we developed a new prefixed 13C tracer analysis by combining a mass spectrometry with 13C tracer analysis, together with a partial enzyme degradation method. This method has been applied to the high-yielding rice to study the subspecies difference on carbon transport in the stems of Japonica- and Indica-dominant high-yielding rice cultivars (Momiroman and Hokuriku 193, respectively) developing in field condition. The data showed that there was a clear difference on starch accumulation/degradation durations functioning in the stems as sink among two cultivars. It has been suggested that starch breakdown in Momiroman occur much later than Hokuriku 193, consistent with the observed patterns of non-structural carbohydrate in the stems. We therefore conclude that the observed yield difference among two subspecies might be attributed to the functional and temporal difference of carbohydrate metabolism in stems.
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