| Project/Area Number |
24248021
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bioproduction chemistry/Bioorganic chemistry
|
|---|
| Research Institution | Shizuoka University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAN Toshiyuki 静岡県立大学, 薬学部, 教授 (10221904)
NAGAI Kaoru 山梨大学, 総合研究部, 准教授 (20340953)
HIRAI Hirofumi 静岡大学, 農学研究科, 教授 (70322138)
MORITA Tatsuya 静岡大学, 農学研究科, 教授 (90332692)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥44,980,000 (Direct Cost: ¥34,600,000、Indirect Cost: ¥10,380,000)
Fiscal Year 2014: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Fiscal Year 2013: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2012: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
|
|---|
| Keywords | キノコ / スギヒラタケ / 急性脳症 / pleurocybellaziridine / 分痔機構 |
|---|
| Outline of Final Research Achievements |
We have a hypothesis of the molecular mechanism of Suguhiratake-acute encephalopathy; the complex between two macromolecules, a lectin (PPL) and B3, destroyed blood brain barrier (BBB), then a low-molecular-weight toxin, pleurocybellaziridine (PA) attacked the brain and caused the unique lesion. To confirm the hypothesis, we obtained the following results.
1) PA was stable when it coexisted with PPL and B3. 2) PA showed toxicity against oligodendrocyte. 3) Genome database of Suguhiratake was established. 4) Expression systems of recombinant PPL and B3 were successfully constructed. 5) The complex of rB3 and rPPL exhibited proteinase activity and destroyed BBB.
|
