Construction of the database of microbial products and its application study
Project/Area Number |
24248022
|
Research Category |
Grant-in-Aid for Scientific Research (A)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Bioproduction chemistry/Bioorganic chemistry
|
Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
OSADA Hiroyuki 独立行政法人理化学研究所, 環境資源科学研究センター, 副センター長 (80160836)
|
Co-Investigator(Renkei-kenkyūsha) |
MUROI Makoto 独立行政法人理化学研究所, 環境資源科学研究センター, 上級研究員 (30261168)
NOGAWA Toshihiko 独立行政法人理化学研究所, 環境資源科学研究センター, 研究員 (40462717)
|
Project Period (FY) |
2012-05-31 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥44,720,000 (Direct Cost: ¥34,400,000、Indirect Cost: ¥10,320,000)
Fiscal Year 2014: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Fiscal Year 2013: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2012: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
|
Keywords | 生物活性物質 / 微生物代謝産物 / プロテオーム / フラクションライブラリー / データベース / MPPlot / ChemProteoBase / NPPlot |
Outline of Final Research Achievements |
In order to overcome disadvantage of natural products as a source for drug screening, we established a technical platform based on chemical biology. In this study, we constructed a fraction library from microbial and plant sources based on basic chromatographic techniques, such as MPLC and HPLC, combined with a database named Natural Products Plot (NPPlot). NPPlot is a distribution map of metabolites, which are plotted as a dot in a three-dimensional area with X-, Y-, and Z-axis for retention time, m/z value, and UV absorption, respectively. Using this database, characteristic metabolites are easily found for each of strain. Furthermore, we developed a drug target identification system based on proteomic analysis by 2-D gel electrophoresis, ChemProteoBase. Isolation of novel natural compounds and prediction of their targets would be accelerated by using our systems, NPPlot and ChemProteoBase. Our research is expected to contribute to drug discovery and progress of bioscience.
|
Report
(4 results)
Research Products
(78 results)
-
-
-
[Journal Article] RK-270A-C, new oxindole derivatives isolated from a microbial metabolites fraction library of Streptomyces sp. RK85-270.2015
Author(s)
Jang JP, Nogawa T, Uramoto M, Okano A, Futamura Y, Shimizu T, Takahashi S, Jang JH, Ahn JS, Osada H.
-
Journal Title
J Antibiot (Tokyo)
Volume: 68
Issue: 4
Pages: 293-295
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
[Journal Article] RK-1355A and B, novel quinomycin derivatives isolated from a microbial metabolites fraction library based on NPPlot screening.2014
Author(s)
Lim CL, Nogawa T, Uramoto M, Okano A, Hongo Y, Nakamura T, Koshino H, Takahashi S, Ibrahim D, Osada H.
-
Journal Title
J Antibiot (Tokyo)
Volume: 67
Issue: 4
Pages: 323-329
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Cell cycle inhibitors targeting Achilles heels of cells2014
Author(s)
Hiroyuki Osada
Organizer
Asian Biological Medicine Summit Forum in Hangzhou: The International Symposium on Natural Products Chemistry and Chemical Biology 2014 & ACBI 2014 Hangzhou Meeting
Place of Presentation
Hangzhou Shujian Hotel (Hangzhou, China)
Year and Date
2014-11-24
Related Report
Invited
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 放線菌由来抗がん性物質 collismycin A の作用標的同定2014
Author(s)
井上 尭, 川谷 誠, 室井 誠, 二村 友史, 青野 晴美, 浦本 昌和, 本郷 やよい, 越野 広雪, 五十嵐 康弘, 安藤 直子, 長田 裕之
Organizer
日本農芸化学会2014年度大会
Place of Presentation
明治大学 生田キャンパス(神奈川県)
Related Report
-
-
-
-
-
-
[Presentation] New OxindoleDerivatives Isolated from the Microbial Metabolite Fraction Library of Streptomyces sp. RK85-2702014
Author(s)
Jun-Pil Jang, Toshihiko Nogawa, Masakazu Uramoto, Akiko Okano, Yushi Futamura, Takeshi Shimizu, Shunji Takahashi, Jae-Hyuk Jang, Jong Seog Ahn, Hiroyuki Osada
Organizer
The 7th Japan-Korea Chemial Biology Symposium
Place of Presentation
Ramada Hotel (Jeju, Korea)
Related Report
-
[Presentation] 抗マラリア原虫薬の開発2013
Author(s)
早瀬 大貴, 渡邉 信元, ヴィエジバ コンスタンティ, 川谷 誠, 北 潔, 長田 裕之
Organizer
第36回日本分子生物学会年会
Place of Presentation
神戸ポートアイランド(兵庫県)
Related Report
-
-
-
[Presentation] Identification of a target for collismycin A, a cytotoxic microbial product, by proteomic profiling2013
Author(s)
Makoto Kawatani, Gyo Inoue, Makoto Muroi, Yushi Futamura, Harumi Aono, Masakazu Uramoto, Yayoi Hongo, Hiroyuki Koshino, Yasuhiro Igarashi, Naoko Takahashi Ando, Hiroyuki Osada
Organizer
AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics
Place of Presentation
Hynes Convention Center (Boston, MA, USA)
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-