Development of forest paleogenetic approaches using ancient pollen grains found in an ice core from mountain glacier
| Project/Area Number |
24248025
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Forest science
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| Research Institution | Tohoku University |
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Principal Investigator |
Suyama Yoshihisa 東北大学, (連合)農学研究科(研究院), 准教授 (60282315)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAZAWA Fumio 大学共同利用機関法人情報・システム研究機構, 新領域融合研究センター, 融合プロジェクト特任研究員 (80432178)
MAKINO Takashi 東北大学, 生命科学研究科, 准教授 (20443442)
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| Research Collaborator |
MATSUKI Yu 東北大学, 大学院農学研究科, 研究支援者
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥44,980,000 (Direct Cost: ¥34,600,000、Indirect Cost: ¥10,380,000)
Fiscal Year 2015: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2014: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2013: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2012: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
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| Keywords | 古代DNA / 遺伝的多様性 / 花粉分析 / 次世代シーケンサー / アイスコア / 集団遺伝 / 分子系統地理 / 針葉樹 |
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| Outline of Final Research Achievements |
We developed basic methods for paleogenetic approaches using conifer pollen grains found in a mountain glacier. First, we developed a protocol for single-pollen DNA analysis using whole-genome amplification (WGA) method. Second, we developed a novel approach that we termed “multiplexed ISSR genotyping by sequencing” (MIG-seq), which is a PCR-based procedure for constructing highly reduced representation libraries involving de novo SNP discovery, and their genotyping using next generation sequencing. Then we applied these methods to pollen samples found in a glacier.
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Report
(5 results)
Research Products
(29 results)
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[Journal Article] DNA analysis for section identification of individual Pinus pollen grains from Belukha glacier, Altai Mountains, Russia2013
Author(s)
Fumio Nakazawa, Jun Uetake, Yoshihisa Suyama, Ryo Kaneko, Nozomu Takeuchi, Koji Fujita, Hideaki Motoyama, Satoshi Imura, Hiroshi Kanda
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Journal Title
Environ. Res. Lett.
Volume: 8
Issue: 1
Pages: 14-32
DOI
Related Report
Peer Reviewed
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