| Budget Amount *help |
¥45,760,000 (Direct Cost: ¥35,200,000、Indirect Cost: ¥10,560,000)
Fiscal Year 2014: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2013: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2012: ¥27,950,000 (Direct Cost: ¥21,500,000、Indirect Cost: ¥6,450,000)
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| Outline of Final Research Achievements |
In this study, we comprehensively analyzed the small RNA involved in RNA interference in fish and shellfish to get basic knowledge about RNAi of the aquatic organisms and utilize the knowledge in the field of fisheries. By the transcriptome using next-generation sequencer, we revealed small RNA expression profile in each tissue of the torafugu (Takifugu rubripes), medaka (Oryzias latipes) and pearl oyster (Pinctada fucata). We found 1420 miRNA species in torafugu, and then found existence of large number of isomiRs for each of miRNA. We had an idea to take advantage of the RNAi in pathogen defense of fish and shellfish. For this, we analyzed the genomic sequences of pathogenic organisms.
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