Elucidation of RNA interference in fish and shellfish and its application for aquaculture and pathogen defense
| Project/Area Number |
24248034
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ASAKAWA Shuichi 東京大学, 農学生命科学研究科, 教授 (30231872)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥45,760,000 (Direct Cost: ¥35,200,000、Indirect Cost: ¥10,560,000)
Fiscal Year 2014: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2013: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2012: ¥27,950,000 (Direct Cost: ¥21,500,000、Indirect Cost: ¥6,450,000)
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| Keywords | RNA干渉 / miRNA / piRNA / torafugu / medaka / pearl oyster / Heterobothrium okamotoi / トランスクリプトーム / 小分子RNA / ヘテロボツリウムオカモトイ / トラフグ / メダカ / miR-499 / アコヤガイ / クドア / 感染防御 / RNA薬 / 寄生虫 / クルマエビ / シーケンシング |
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| Outline of Final Research Achievements |
In this study, we comprehensively analyzed the small RNA involved in RNA interference in fish and shellfish to get basic knowledge about RNAi of the aquatic organisms and utilize the knowledge in the field of fisheries. By the transcriptome using next-generation sequencer, we revealed small RNA expression profile in each tissue of the torafugu (Takifugu rubripes), medaka (Oryzias latipes) and pearl oyster (Pinctada fucata). We found 1420 miRNA species in torafugu, and then found existence of large number of isomiRs for each of miRNA. We had an idea to take advantage of the RNAi in pathogen defense of fish and shellfish. For this, we analyzed the genomic sequences of pathogenic organisms.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Dramatic improvement in genome assembly achieved using doubled-haploid genomes.2014
Author(s)
Zhang H, Tan E, Suzuki Y, Hirose Y, Kinoshita S, Okano H, Kudoh J, Shimizu A, Saito K, Watabe S, Asakawa S.
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Journal Title
Sci Rep.
Volume: 4
Pages: 6780-6780
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Draft genome sequence of Aeromonas hydrophila strain Ae34, isolated from a koi carp showing signs of haemorrhagic septicaemia,2013
Author(s)
agoda, S.S.S.De S., Engkong, T., Kinoshita, S., Watabe, S., Asakawa, S.
Organizer
The 36th Annual meeting of the Molecular Biology Society of Japan
Place of Presentation
Kobe, Japan
Related Report
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