Identification of human bone marrow niche to support HSCs

• Project/Area Number: 24249056
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: The University of Tokyo
• Principal Investigator: NAKAUCHI Hiromitsu 東京大学, 医科学研究所, 教授 (40175485)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥44,460,000 (Direct Cost: ¥34,200,000、Indirect Cost: ¥10,260,000); Fiscal Year 2014: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000); Fiscal Year 2013: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000); Fiscal Year 2012: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
• Keywords: 骨髄ニッチ / TGF-beta / 休眠状態

Outline of Final Research Achievements

This project describes identification of human bone marrow niche to support HSCs. We hypothesized that identification of BM niche leads us to manipulate the function of human HSPCs in vivo and in vitro. Given that TGF-beta induces human HSPC hibernation, we analyzed the area where TGF is activated. Our result clearly showed that GFAP+ cells activate TGF and induce hibernation of human HSPCs in mouse BM. These findings were confirmed by the fact that nerve denervation reduces the number of human HSPCs in human BM. Furthermore, We first report suggesting an association between neural and hematopoietic systems.In this paper, we first described an innovative strategy to generate large numbers of HSCs from pluripotent stem cells. More specifically, we established in vivo differentiation system generating fully functional HSCs from mouse induced pluripotent stem cells. Using this system, we also succeeded in generating human HSCs from human iPSCs.

Research Products

• [Journal Article] Effect of donor chimerism to reduce the level of glycosaminoglycans following bone marrow transplantation in a murine model of mucopolysaccharidosis type II (2015)
  • Author(s): Yokoi K, Akiyama K, Kaneshiro E, Higuchi T, Shimada Y, Kobayashi H, Akiyama M, Otsu M, Nakauchi H, Ohashi T, Ida H
  • Journal Title: J Inherit Metab Dis Volume: 38 Pages: 333-340
  • DOI: 10.1007/s10545-014-9800-x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Screening of Drugs to Treat 8p11 Myeloproliferative Syndrome Using Patient-Derived Induced Pluripotent Stem Cells with Fusion Gene CEP110-FGFR1. (2015)
  • Author(s): Yamamoto S, Otsu M, Matsuzaka E, Konishi C, Takagi H, Hanada S, Mochizuki S, Nakauchi H, Imai K, Tsuji K, Ebihara Y
  • Journal Title: PloS one Volume: 10
  • DOI: 10.1371/journal.pone.0120841
  • Peer Reviewed / Open Access
• [Journal Article] Circulating cells contribute to cardiomyocyte regeneration after injury (2015)
  • Author(s): Wu JM, Hsueh YC, Ch'ang HJ, Luo CY, Wu LW, Nakauchi H, Hsieh PC
  • Journal Title: Circ Res Volume: 116 Pages: 633-41
  • DOI: 10.1161/circresaha.116.304564
  • Peer Reviewed / Open Access
• [Journal Article] Inhibition of plasmin attenuates murine acute graft-versus-host disease mortality by suppressing the matrix metalloproteinase-9-dependent inflammatory cytokine storm and effector cell trafficking (2015)
  • Author(s): A Sato, C Nishida, K Sato-Kusubata, M Ishihara, Y Tashiro, I Gritli, H Shimazu, S Munakata, H Yagita, K Okumura, Y Tsuda, Y Okada, A Tojo, H Nakauchi, S Takahashi, B Heissig and K Hattori
  • Journal Title: Leukemia Volume: 29 Pages: 145-156
  • DOI: 10.1038/leu.2014.151
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effective treatment against severe graft-versus-host disease with allele-specific anti-HLA monoclonal antibody in a humanized mouse model. (2015)
  • Author(s): Nakauchi Y, Yamazaki S, Napier SC, Usui J, Ota Y, Takahashi S, Watanabe N, Nakauchi H
  • Journal Title: Exp Hematol Volume: 43 Pages: 79-88
  • DOI: 10.1016/j.exphem.2014.10.008
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Quantification of adult T-cell leukemia/lymphoma cells using simple four-color flow cytometry. (2015)
  • Author(s): Ishigaki T, Zaike Y, Nojima M, Kobayashi S, Ohno N, Uchimaru K, Tojo A, Nakauchi H, Watanabe N
  • Journal Title: Clin Chem Lab Med Volume: 53(1) Pages: 85-93
  • DOI: 10.1515/cclm-2014-0183
  • Peer Reviewed
• [Journal Article] T cell-restricted T-bet overexpression induces aberrant hematopoiesis of myeloid cells and impairs function of lung macrophages in the lung. (2015)
  • Author(s): Iriguchi S, Kikuchi N, Kaneko S, Noguchi E, Morishima Y, Matsuyama M, Yoh K, Takahashi S, Nakauchi H, Ishii Y
  • Journal Title: Blood Volume: 125 Pages: 370-382
  • DOI: 10.1182/blood-2014-05-575225
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Bone marrow Schwann cells induce hematopoietic stem cell hibernation. (2014)
  • Author(s): Yamazaki S, Nakauchi H
  • Journal Title: Int J Hematol Volume: 99 Pages: 695-698
  • DOI: 10.1007/s12185-014-1588-9
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Five-lineage clonal analysis of hematopoietic stem/progenitor cells (2014)
  • Author(s): Yamamoto R, Morita Y, Nakauchi H
  • Journal Title: Methods in molecular biology Volume: 1185 Pages: 237-45
  • DOI: 10.1007/978-1-4939-1133-2_16
  • Peer Reviewed
• [Journal Article] Impaired hematopoietic differentiation of RUNX1-mutated induced pluripotent stem cells derived from FPD/AML patients. Leukemia, (2014)
  • Author(s): Sakurai M, Kunimoto H, Watanabe N, Fukuchi Y, Yuasa S, Yamazaki S, Nishimura T, Sadahira K, Fukuda K, Okano H, Nakauchi H, Morita Y, Matsumura I, Kudo K, Ito E, Ebihara Y, Tsuji K, Harada Y, Harada H, Okamoto S, Nakajima H
  • Journal Title: Leukemia. Volume: 28 Pages: 2344-2354
  • DOI: 10.1038/leu.2014.136
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Multicolor staining of globin subtypes reveals impaired globin switching during erythropoiesis in human pluripotent stem cells. (2014)
  • Author(s): Ochi K, Takayama N, Hirose S, Nakahata T, Nakauchi H, Eto K
  • Journal Title: Stem Cells Transl Med. Volume: 3 Pages: 792-800
  • DOI: 10.5966/sctm.2013-0216
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Expandable megakaryocyte cell lines enable clinically applicable generation of platelets from human induced pluripotent stem cells. (2014)
  • Author(s): Nakamura S, Takayama N, Hirata S, Seo H, Endo H, Ochi K, Fujita KI, Koike T, Harimoto KI, Dohda T, Watanabe A, Okita K, Takahashi N, Sawaguchi A, Yamanaka S, Nakauchi H, Nishimura S, Eto K.
  • Journal Title: Cell Stem Cell Volume: 14 Pages: 535-48
  • DOI: 10.1016/j.stem.2014.01.011
  • Peer Reviewed / Open Access
• [Journal Article] The Tif1β-Hp1 system maintains transcriptional integrity of hematopoietic stem cells. (2014)
  • Author(s): Miyagi S, Koide S, Saraya A, Wendt GR, Oshima M, Konuma T, Yamazaki S, Mochizuki-Kashio M, Nakajima-Takagi Y, Wang C, Chiba T, Kitabayashi I, Nakauchi H, Iwama A.
  • Journal Title: Stem cell reports Volume: 2 Pages: 145-52
  • DOI: 10.1016/j.stemcr.2013.12.008
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Haploinsufficiency of Sf3b1 leads to compromised stem cell function but not tomyelodysplasia (2014)
  • Author(s): Matsunawa M, Yoshida K
  • Journal Title: Leukemia. Volume: (Epub ahead of print) Pages: 1844-1850
  • DOI: 10.1038/leu.2014.73
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Stage-specific roles for Cxcr4 signaling in murine hematopoietic stem/progenitor cells in the process of bone marrow repopulation (2014)
  • Author(s): Lai CY, Yamazaki S, Okabe M, Suzuki S, Maeyama Y, Iimura Y, Onodera M, Kakuta S, Iwakura Y, Nojima M, Otsu M, Nakauchi H.
  • Journal Title: Stem Cells Volume: - Pages: 1929-42
  • DOI: 10.1002/stem.1670
  • Peer Reviewed
• [Journal Article] Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies. (2014)
  • Author(s): Kazuki Y, Yakura Y, Abe S, Osaki M, Kajitani N, Kazuki K, Takehara S, Honma K, Suemori H, Yamazaki S, Sakuma T, Toki T, Shimizu R, Nakauchi H, Yamamoto T, Oshimura M
  • Journal Title: Sci Rep Volume: 4 Pages: 6136-6136
  • DOI: 10.1038/srep06136
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Gene targeting study reveals unexpected expression of brain-expressed X-linked 2 in endocrine and tissue stem/progenitor cells in mice. (2014)
  • Author(s): Keiichi Ito, Satoshi Yamazaki, Ryo Yamamoto, Yoko Tajima, Ayaka Yanagida, Toshihiro Kobayashi, Megumi Kato-Itoh, Shigeru Kakuta, Yoichiro Iwakura, Hiromitsu Nakauchi, Akihide Kamiya,
  • Journal Title: J Biol Chem. Volume: 289(43) Pages: 29892-298911
  • DOI: 10.1074/jbc.m114.580084
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Clock gene Bmal1 is dispensable for intrinsic properties of murine hematopoietic stem cells (2014)
  • Author(s): Ieyasu A, Tajima Y, Shimba S, Nakauchi H, Yamazaki S
  • Journal Title: Journal of negative results in biomedicine Volume: 13 Pages: 4-4
  • DOI: 10.1186/1477-5751-13-4
  • Peer Reviewed / Open Access
• [Journal Article] Generation of induced pluripotent stem cells derived from primary and secondary myelofibrosis patient samples. (2014)
  • Author(s): Hosoi M, Kumano K, Taoka K, Arai S, Kataoka K, Ueda K, Kamikubo Y, Takayama N, Otsu M, Eto K, Nakauchi H, Kurokawa M
  • Journal Title: Experimental Hematology Volume: (in press) Pages: 816-25
  • DOI: 10.1016/j.exphem.2014.03.010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A Chemical Probe That Labels Human Pluripotent Stem Cells. (2014)
  • Author(s): Hirata N, Nakagawa M, Fujibayashi Y, Yamauchi K, Murata A, Minami I, Tomioka M, Kondo T, Kuo TF, Endo H, Inoue H, Sato S, Ando S, Kawazoe Y, Aiba K, Nagata K, Kawase E, Chang YT, Suemori H, Eto K, Nakauchi H, Yamanaka S, Nakatsuji N, Ueda K, Uesugi M
  • Journal Title: Cell Rep Volume: 6 Pages: 1165-1174
  • DOI: 10.1016/j.celrep.2014.02.006
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The generation of induced pluripotent stem cells (iPSCs) from patients with infantile and late-onset types of Pompe disease and the effects of treatment with acid-α-glucosidase in Pompe's iPSCs. (2014)
  • Author(s): Higuchi T, Kawagoe S, Otsu M, Shimada Y, Kobayashi H, Hirayama R, Eto K, Ida H, Ohashi T, Nakauchi H, Eto Y
  • Journal Title: Mol Genet Metab Volume: 112 Pages: 44-48
  • DOI: 10.1016/j.ymgme.2014.02.012
  • Peer Reviewed
• [Journal Article] Nov/ccn3 regulates long-term repopulating activity of murine hematopoietic stem cells via integrin αvβ3 (2014)
  • Author(s): Ishihara, J., Umemoto, T., Yamato, M., Shiratsuchi, Y., Takaki, S., Petrich, B.G., Nakauchi, H., Eto, K., Kitamura, T., and Okano T.
  • Journal Title: International Journal of Hematology Volume: 99 Pages: 393-406
  • DOI: 10.1007/s12185-014-1534-x
  • Peer Reviewed
• [Journal Article] Homeodomain transcription factor meis1 is a critical regulator of adult bone marrow hematopoiesis (2014)
  • Author(s): Ariki R, Morikawa S, Mabuchi Y, Suzuki S, Nakatake M, Yoshioka K, Hidano S, Nakauchi H, Matsuzaki Y, Nakamura T, Goitsuka R.
  • Journal Title: PLOS ONE Volume: 9 Pages: 87646-87646
  • DOI: 10.1371/journal.pone.0087646
  • Peer Reviewed / Open Access
• [Journal Article] Enzyme augmentation therapy enhances the therapeutic efficacy of bone marrow transplantation in mucopolysaccharidosis type II mice. (2014)
  • Author(s): Akiyama K, Shimada Y, Higuchi T, Ohtsu M, Nakauchi H, Kobayashi H, Fukuda T, Ida H, Eto Y, Crawford BE, Brown JR, Ohashi T
  • Journal Title: Mol Genet Metab. Volume: 111 Pages: 139-46
  • DOI: 10.1016/j.ymgme.2013.09.013
  • Peer Reviewed
• [Journal Article] Generation of Col2a1-EGFP-iPS cells for monitoring chondrogenic differentiation. (2014)
  • Author(s): Saito T, Yano F, Mori D, Ohba S, Hojo H, Otsu M, Eto K, Nakauchi H, Tanaka S, Chung UI, Kawaguchi H.
  • Journal Title: PLoS ONE Volume: 8
  • DOI: 10.1371/journal.pone.0074137
  • Peer Reviewed
• [Journal Article] Derivation of Embryonic Stem Cell Lines from Parthenogenetically Developing Rat Blastocysts. (2014)
  • Author(s): Hirabayashi M, Goto T, Tamura C, Sanbo M, Hara H, Kato-Itoh M, Sato H, Kobayashi T, Nakaushi H, Hochi S.
  • Journal Title: Stem Cell Dev. Volume: VOL.23 Pages: 107-114
  • DOI: 10.1089/scd.2013.0200
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Immortalization of Erythroblasts by c-MYC and BCL-XL Enables Large-Scale Erythrocyte Production from Human Pluripotent Stem Cells (2013)
  • Author(s): Hirose, S., N. Takayama, S. Nakamura, K. Nagasawa, K. Ochi, S. Hirata, S. Yamazaki, T. Yamaguchi, M. Otsu, S. Sano, N. Takahashi, A. Sawaguchi, M. Ito, T. Kato, H. Nakauchi, and K. Eto.
  • Journal Title: Stem cell reports Volume: 1 Pages: 499-508
  • DOI: 10.1016/j.stemcr.2013.10.010
  • Peer Reviewed
• [Journal Article] Profiling of microRNA in human and mouse ES and iPS cells reveals overlapping but distinct microRNA expression patterns (2013)
  • Author(s): Razak SR, Ueno K, Takayama N, Nariai N, Nagasaki M, Saito R, Koso H, Lai CY, Murakami M, Tsuji K, Michiue T, Nakauchi H, Otsu M, Watanabe S.
  • Journal Title: Plos One Volume: 8
  • DOI: 10.1371/journal.pone.0073532
  • Peer Reviewed / Open Access
• [Journal Article] Top-down motif engineering of a cytokine receptor for directing ex vivo expansion of hematopoietic stem cells (2013)
  • Author(s): Saka K, Kawahara M, Teng J, Otsu M, Nakauchi H, Nagamune T.
  • Journal Title: J Biotechnol Volume: 168 Pages: 659-65
  • DOI: 10.1016/j.jbiotec.2013.09.012
  • Peer Reviewed
• [Journal Article] Do pluripotent stem cells exist in adult mice as very small embryonic stem cells? (2013)
  • Author(s): Miyanishi M, Mori Y, Seita J, Chen JY, Karten S, Chan CK, Nakauchi H, Weissman IL.
  • Journal Title: Stem Cell Reports. Volume: 1 Pages: 198-208
  • DOI: 10.1016/j.stemcr.2013.07.001
  • Peer Reviewed
• [Journal Article] Clonal analysis unveils self-renewing lineage-restricted progenitors generated directly from hematopoietic stem cells (2013)
  • Author(s): Yamamoto R, Morita Y, Ooehara J, Hamanaka S, Onodera M, Rudolph KL, Ema H, Nakauchi H.
  • Journal Title: CELL Volume: 154 Pages: 1112-1126
  • DOI: 10.1016/j.cell.2013.08.007
  • Peer Reviewed
• [Journal Article] Recurrent mutations in multiple components of the cohesin complex inmyeloid neoplasms (2013)
  • Author(s): Kon A, Yoshida K
  • Journal Title: Nature Genetics Volume: 45 Pages: 1232-1237
  • DOI: 10.1038/ng.2731
  • Peer Reviewed
• [Journal Article] An in vitro expansion system for generation of human iPS cell-derived hepatic progenitor-like cells exhibiting a bipotent differentiation potential. (2013)
  • Author(s): Yanagida A, Ito K, Chikada H, Nakauchi H, Kamiya A.
  • Journal Title: Plos One Volume: 8
  • DOI: 10.1371/journal.pone.0067541
  • Peer Reviewed
• [Journal Article] Congenital amegakaryocytic thrombocytopenia iPS cells exhibit defective MPL-mediated signaling (2013)
  • Author(s): Shinji Hirata, Naoya Takayama,Ryoko Jono-Ohnishi, Hiroshi Endo, Sou Nakamura, Takeaki Dohda, Masanori Nishi, Yuhei Hamazaki, Ei-ichi Ishii, Shin Kaneko, Makoto Otsu, Hiromitsu Nakauchi, Shinji Kunishima, and Koji Eto.
  • Journal Title: The Journal of clinical investigation Volume: 123 Pages: 3802-14
  • DOI: 10.1172/jci64721
  • Peer Reviewed
• [Journal Article] Two differential flows in a bioreactor promoted platelet generation from human pluripotent stem cell-derived megakaryocytes (2013)
  • Author(s): Nakagawa Y, Nakamura S, Nakajima M, Endo H, Dohda T, Takayama N, Nakauchi H, Arai F, Fukuda T, Eto K.
  • Journal Title: Experimental Hematology Volume: 41 Pages: 742-748
  • DOI: 10.1016/j.exphem.2013.04.007
  • Peer Reviewed
• [Journal Article] Generation of engraftable hematopoietic stem cells from induced pluripotent stem cells by way of teratoma formation (2013)
  • Author(s): Suzuki N, Yamazaki S, Yamaguchi T, Okabe M, Masaki H, Takaki S, Otsu M, Nakauchi H.
  • Journal Title: Mol Ther Volume: 21 Pages: 1424-1431
  • DOI: 10.1038/mt.2013.71
  • Peer Reviewed
• [Journal Article] Generation of transgenic mouse line expressing Kusabira Orange throughout body, including erythrocytes, by random segregation of provirus method. (2013)
  • Author(s): Hamanaka S, Ooehara J, Morita Y, Ema H, Takahashi S, Miyawaki A, Otsu M, Yamaguchi T, Onodera M, Nakauchi H
  • Journal Title: Biochemical and biophysical research communications Volume: i435(4) Pages: 586-591
  • DOI: 10.1016/j.bbrc.2013.05.017
  • Peer Reviewed
• [Journal Article] Generation of induced pluripotent stemcells fromprimary chronic myelogenous leukemia patient samples. (2013)
  • Author(s): Kumano K
  • Journal Title: Curr Gene Ther. Volume: 119 Pages: 6234-42
  • DOI: 10.1182/blood-2011-07-367441
  • Peer Reviewed
• [Journal Article] Stem cell therapy: an exercise in patience and prudence. Stem cell therapy: an exercise in patience and prudence. (2013)
  • Author(s): Lin HT, Otsu M, Nakauchi H
  • Journal Title: Biological sciences Volume: 368 Pages: 20110334-20110334
  • DOI: 10.1098/rstb.2011.0334
  • Peer Reviewed
• [Journal Article] Role of SOX17 in hematopoietic development fromhuman embryonic stemcells. (2013)
  • Author(s): Nakajima-Takagi Y, Osawa M, Oshima M, Takagi H, Miyagi S, Endoh M, Endo TA, Takayama N, Eto K, Toyoda T, Koseki H, Nakauchi H, Iwama A.
  • Journal Title: Blood Volume: 121 Pages: 447-458
  • DOI: 10.1182/blood-2012-05-431403
  • Peer Reviewed
• [Journal Article] Generation of rejuvenated antigen-specific T cells by pluripotency reprogramming and dedifferentiation. (2013)
  • Author(s): Nishimura T, Koseki H, Nakauchi H. 他
  • Journal Title: Cell StemCell. Volume: 12 Pages: 114-126
  • DOI: 10.1016/j.stem.2012.11.002
  • Peer Reviewed
• [Journal Article] MT1-MMP plays a critical role in hematopoiesis by regulating HIF-mediated chemokine/cytokine gene transcription within niche cells (2012)
  • Author(s): Chiemi Nishida, Kaori Kusubata, Yoshihiko Tashiro, Ismael Gritli, Aki Sato, Makiko Ohki-Koizumi, Yohei Morita, Makoto Nagano, Takeharu Sakamoto, Naohiko Koshikawa, Takahiro Kuchimaru, Shinae Kizaka-Kondoh, Motoharu Seiki, Hiromitsu Nakauchi, Beate Heissig, and Koichi Hattori.
  • Journal Title: Blood. Volume: 119 Pages: 5405-16
  • DOI: 10.1182/blood-2011-11-390849
  • Peer Reviewed
• [Journal Article] nhibition of PAI-1 induces neutrophil-driven neoangiogenesis and promotes tissue regeneration viaproduction of angiocrine factors in mice (2012)
  • Author(s): Yoshihiko Tashiro, Chiemi Nishida, Kaori Sato-Kusubata, Makiko Ohki-Koizumi, Makoto Ishihara, Aki Sato, Ismael Gritli, Hiromitsu Komiyama, Yayoi Sato, Takashi Dan, Toshio Miyata, Ko Okumura, Yuichi Tomiki, Kazuhiro Sakamoto, Hiromitsu Nakauchi, Beate Heissig, and Koichi Hattori.
  • Journal Title: blood Volume: 119 Pages: 6382-6393
  • DOI: 10.1182/blood-2011-12-399659
  • Peer Reviewed
• [Journal Article] Bmi1 confers resistance to oxidative stress on hematopoietic stem cells. (2012)
  • Author(s): Nakamura S, Oshima M, Yuan J, Saray a A, Miyagi S, Konuma T, Yamazaki S, Osawa M, Nakauchi H, Koseki H, Iwama A.
  • Journal Title: PLoS ONE Volume: 7(5)
  • DOI: 10.1371/journal.pone.0036209
  • Peer Reviewed
• [Journal Article] Development of an all-in-one inducible lentiviral vector for gene specific analysis of reprogramming. (2012)
  • Author(s): Yamaguchi T, Hamanaka S, Kamiya A, Okabe M, Kawarai M, Wakiyama Y, Umino A, Hayama T, Sato H, Lee YS, Kato-Itoh M, Masaki H, Kobayashi T, Yamazaki S, Nakauchi H.
  • Journal Title: PLoS One Volume: 7
  • DOI: 10.1371/journal.pone.0041007
  • Peer Reviewed
• [Journal Article] Rapid T-cell chimerism switch and memory T-cell expansion are associated with pre-engraftment immune reaction early after cord blood transplantation. (2012)
  • Author(s): Matsuno N, Yamamoto H, Watanabe N, Uchida N, Ota H, Nishida A, Ikebe T, Ishiwata K, Nakano N, Tsuji M, Asano-Mori Y, Izutsu K, Masuoka K, Wake A, Yoneyama A, Nakauchi H, Taniguchi S.
  • Journal Title: Br J Haematol Volume: 160 Pages: 255-8
  • DOI: 10.1111/bjh.12097
  • Peer Reviewed
• [Presentation] Myeloid-biased hematopoiesis in aged mouse bone marrow (2015)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: Japan-US Hematology conference
  • Place of Presentation: Hawaii, U.S.
  • Year and Date: 2015-03-16 – 2015-03-18
  • Invited
• [Presentation] The Stem Cell Niche (2015)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: Gordon Research Conference
  • Place of Presentation: Ventura Beach Marriott, Ventura, CA. US
  • Year and Date: 2015-02-15 – 2015-02-20
  • Invited
• [Presentation] In vivo generation of hematopoietic stem cells from iPSCs (2014)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: JST-CIHR Epigenetics F2F Meeting
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2014-10-29
  • Invited
• [Presentation] Single cell transplantation and clonal analysis of hematopoietic stem cells: Exploring heterogeneity and hierarchy (2014)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: Till&McCulloch Meetings
  • Place of Presentation: Ottawa, Canada
  • Year and Date: 2014-10-29
  • Invited
• [Presentation] "Novel non-stepwise early differentiation pathways of HSCs revealed by 5-lineage in vivo tracing" (2013)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: Gordon Research Conference “Stem Cells & Cancer”
  • Place of Presentation: Les Diablerets, Switzerland
  • Invited
• [Presentation] In Vivo Clonal Analysis of Hematopoietic Stem Cells Unveils Novel Myeloid Bypass Differentiation Pathways. (2013)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: 2013 USA-Japan Science Conference
  • Place of Presentation: Hawaii, USA
  • Invited
• [Presentation] iPS technology and its future therapeutic potentials. (2012)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: The 5th Dutch Society for Stem Cell Research Meeting (DSSCR)
  • Place of Presentation: Amsterdam, Holland
  • Invited
• [Presentation] Isolation, Clonal Characterization and Hibernation of Hematopoietic Stem Cells. (2012)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: Wellcome Trust Centre for Stem Cell Research, University of Cambridge
  • Place of Presentation: Cambridge, England
  • Invited
• [Presentation] Generation of functional organs from pluripotent stem cells. (2012)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: ISSCR 10th Annual Meeting
  • Place of Presentation: Yokohama, Japan
  • Invited
• [Presentation] Generation of functional organs from pluripotent stem cells. (2012)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: Lecture Theatre, Department of Biochemistry, Cambridge Stem Cell Club
  • Place of Presentation: Cambridge, England
  • Invited
• [Presentation] iPS technology and its future therapeutic potentials. (2012)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: The Sanger Institute
  • Place of Presentation: Cambridge, England
  • Invited
• [Presentation] iPS technology and its potential for future medicine. (2012)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: University of Geneva Hospital
  • Place of Presentation: Geneva, Switzerland
  • Invited
• [Presentation] Stem cell biology and its potentials for future medicine. (2012)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: University of Zurich
  • Place of Presentation: Zurich, Switzerland
  • Invited
• [Presentation] iPS technology and its potential for future medicine. (2012)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: The Babraham Institute
  • Place of Presentation: Cambridge, England
  • Invited
• [Presentation] Stem Cell Niche and TGF-beta signaling. (2012)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: EMBL Conference, Stem Cells in Cancer and Regenerative Medicine
  • Place of Presentation: Edinburgh, England
  • Invited
• [Presentation] Hibernation of Hematopoietic Stem Cells in the Bone Marrow Niche. (2012)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: The University of Edinburgh
  • Place of Presentation: Edinburgh, England
  • Invited
• [Presentation] Glial cell regulation of hematopoietic stem cell hibernation in the bone marrow niche. (2012)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: ESGCT/SFTCG Congress
  • Place of Presentation: Versailles, France
  • Invited
• [Presentation] Rat-mouse interspecific chimeras for study of organogenesis and elucidation of xenogenic barrier. (2012)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: Joint meeting of ISD and NIRM
  • Place of Presentation: Amsterdam, Netherland
  • Invited
• [Presentation] Non-myelinating Schwann cells in the mouse bone marrow niche maintain hematopoietic stem cell hibernation through TGF-β signaling. (2012)
  • Author(s): Hiromitsu Nakauchi
  • Organizer: Cold Spring Harbor Asia Conferences
  • Place of Presentation: Suzhou, China
  • Invited
• [Presentation] Exploring heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment
  • Author(s): 中内啓光
  • Organizer: 日本分子生物学会シンポジウム
  • Place of Presentation: 兵庫
  • Invited
• [Book] 幹細胞研究と再生医療 (2013)
  • Author(s): 中内啓光（編集）
  • Total Pages: 238
  • Publisher: 南山堂
• [Book] 直伝！フローサイトメトリー (2013)
  • Author(s): 中内啓光（監修）清田純（編集）
  • Total Pages: 278
  • Publisher: 羊土社
• [Patent(Industrial Property Rights)] 造血幹細胞を減少させる組成物およびその製造法 (2014)
  • Inventor(s): 中内 啓光、山崎 聡、田矢 祐規
  • Industrial Property Rights Holder: 国立大学法人 東京大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-11-25
  • Application Year: 2014