Mechanisms of integration and elimination of adult-born olfactory bulb neurons during olfactory behavior and sleep

• Project/Area Number: 24300118
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: The University of Tokyo
• Principal Investigator: YAMAGUCHI Masahiro 東京大学, 医学(系)研究科(研究院), 講師 (60313102)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2012: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: 嗅球 / 神経新生 / シナプス構造 / 睡眠 / 末梢性入力 / トップダウン性入力 / 中枢性入力

Outline of Final Research Achievements

In the olfactory bulb (OB), neurogenesis continues even in adult. We previously found that selection of new OB neurons between survival and death is promoted during food eating and subsequent sleep.
In this project, we have revealed that 1) top-down synaptic inputs from the olfactory cortex to the OB play crucial roles in the cell selection 2) structure of dendritic protrusions of new OB neurons drastically changes during feeding-sleep and 3) the cell selection is also promoted during fear responses of mice depending on the top-down inputs from the olfactory cortex. These observations enabled us to understand the mechanism of new OB neuron selection with regard to the integration of bottom-up sensory inputs from the periphery and top-down inputs from the olfactory cortex, which can occur during various behaviors of animals.

Research Products

• [Journal Article] Nestin(+) tissue-resident multipotent stem cells contribute to tumor progression by differentiating into pericytes and smooth muscle cells resulting in blood vessel remodeling (2014)
  • Author(s): Klein D, Meissner N, Kleff V, Jastrow H, Yamaguchi M, Ergun S, Jendrossek V
  • Journal Title: Frontiers in Oncology Volume: 4
  • DOI: 10.3389/fonc.2014.00169
  • Peer Reviewed / Open Access
• [Journal Article] Top-down inputs from the olfactory cortex in the postprandial period promote elimination of granule cells in the olfactory bulb (2014)
  • Author(s): Komano-Inoue S., Manabe H. Ota, M. Kusumoto-Yoshida I., Yokoyama T, Mori, K. and Yamaguchi M.
  • Journal Title: European Journal of Neuroscience Volume: 40 Pages: 2724-2733
  • DOI: 10.1111/ejn.12679
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Critical periods in adult neurogenesis and possible clinical utilization of new neurons. (2014)
  • Author(s): Yamaguti, M. and Mori, K
  • Journal Title: Frontiers in Neuroscience Volume: 8 Pages: 1-3
  • DOI: 10.3389/fnins.2014.00177
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Continuous postnatal neurogenesis contributes to formation of the olfactory bulb neural circuits and flexible olfactory associative learning (2014)
  • Author(s): Sakamoto, M., Ieki, N., Miyoshi, G., Mochimaru, D., Miyachi, H., Imura, T., Yamaguchi, M., Fishell, G., Mori, K., Kageyama, R. and Imayoshi, I.
  • Journal Title: The Journal of Neuroscience Volume: 34 Pages: 5788-5788
  • DOI: 10.1523/jneurosci.0674-14.2014
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Reorganization of neuronal circuits of the central olfactory system during postprandial sleep (2013)
  • Author(s): Yamaguchi M, Manabe H, Murata K, and Mori K
  • Journal Title: Front. Neural Circuits Volume: 7 Pages: 132-132
  • DOI: 10.3389/fncir.2013.00132
  • Peer Reviewed
• [Journal Article] Visualization of odor-induced neuronal activity by immediate early gene expression. (2012)
  • Author(s): Bepari AK, Watanabe K, Yamaguchi M, Tamamaki N, Takebayashi H.
  • Journal Title: BMC Neuroscience Volume: 13 Pages: 140-140
  • DOI: 10.1186/1471-2202-13-140
  • Peer Reviewed
• [Presentation] Understanding the new neurons in the olfactory bulb within the large olfactory neuronal network (2015)
  • Author(s): Masahiro Yamaguchi
  • Organizer: 第１２０回日本解剖学会第９２回日本生理学会合同大会
  • Place of Presentation: 神戸国際会議場・神戸
  • Year and Date: 2015-03-23
• [Presentation] 嗅覚系のニューロン新生と嗅覚神経回路の可塑性 (2014)
  • Author(s): 山口正洋
  • Organizer: 第７回香りと味に関する産学フォーラム
  • Place of Presentation: 東京大学・東京
  • Year and Date: 2014-11-07
  • Invited
• [Presentation] Activity-dependent integration of adult-born granule cells into neuronal circuits of the mouse olfactory bulb (2014)
  • Author(s): Naritsuka H, Mori K, Yamaguchi M
  • Organizer: 第３７回日本神経科学大会
  • Place of Presentation: パシフィコ横浜・横浜
  • Year and Date: 2014-09-12
• [Presentation] Synchronized top-down inputs from the olfactory cortex during postprandial sleep participate in the enhanced cell death of adult-born granule cells in the olfactory bulb (2013)
  • Author(s): Sayaka Komano, Hiroyuki Manabe, Mizuho Ota, Ikue Kusumoto-Yoshida, Takeshi Yokoyama, Kensaku Mori, Masahiro Yamaguchi
  • Organizer: 43th SfN annual meeting “NEUROSCIENCE 2013”
  • Place of Presentation: San Diego, California, USA
• [Presentation] Adult-born granule cells form synapses at distinct subcellular domains of projection neurons in the mouse olfactory bulb (2013)
  • Author(s): Hiromi Naritsuka, Kensaku Mori, Masahiro Yamaguchi
  • Organizer: Neurogenesis 2013
  • Place of Presentation: Matsushima, Miyagi, Japan
• [Presentation] 成体嗅覚系における神経細胞の入れ替わり機構 (2013)
  • Author(s): 山口正洋
  • Organizer: 第８６回日本生化学会
  • Place of Presentation: パシフィコ横浜、横浜
• [Presentation] 食後睡眠時に増加する嗅球顆粒細胞の除去における嗅皮質からのトップダウン入力の役割 (2013)
  • Author(s): 駒野-井上清香、眞部寛之、太田瑞穂、楠本-吉田郁恵、横山健、森憲作、山口正洋
  • Organizer: 第３５回日本神経科学大会
  • Place of Presentation: 国立京都国際会館、京都
• [Presentation] 成体で新生した顆粒細胞が嗅球投射ニューロンの異なる細胞領域に形成するシナプス (2013)
  • Author(s): 成塚裕美、森憲作、山口正洋
  • Organizer: 第３５回日本神経科学大会
  • Place of Presentation: 国立京都国際会館、京都
• [Presentation] Interaction between olfactory bulb and olfactory cortex in the reorganization of the olfactory circuit (2012)
  • Author(s): 山口正洋
  • Organizer: 第25回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2012-09-18
• [Presentation] Experience-dependent reorganization of the olfactory circuitry during sleep state (2012)
  • Author(s): 山口正洋
  • Organizer: 第37回日本睡眠学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2012-06-29
• [Book] Interneurons in the olfactory bulb: roles in the plasticity of olfactory information processing. In: “The olfactory system: from odor molecules to motivational behaviors” (Ed.) Mori K (2014)
  • Author(s): Masahiro Yamaguchi
  • Total Pages: 36
  • Publisher: Springer