| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2014: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Outline of Final Research Achievements |
In this study, we generated two novel knock-in mouse lines, which are designed to express Atoh1 and Ptf1a ectopically in the VZ and RL, respectively. In those mutant mice, we observed glutamatergic and GABAergic neurons ectopically produced from Atoh1-expressing VZ and Ptf1a-expressing RL, respectively, suggesting that spatial identities of cerebellar neuron progenitors are regulated by Atoh1 and Ptf1a.
Within the cerebellar VZ at early stages, we find two types of progenitors; Olig2-expressing Purkinje cell progenitors (PCPs) and Gsx1-expressing Pax2-positive interneuron progenitors (PIPs). As development proceeds, PCPs gradually become PIPs starting from ventral to dorsal. The temporal identity transition of cerebellar GABAergic neuron progenitors from PCPs to PIPs is negatively regulated by Olig2 and positively by Gsx1.
These findings contribute to understanding spatiotemporal control of neuronal progenitor identities by transcription factors.
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