HCN4-luciferase knock-in mouse is a new tool for pacemaker cell reprogramming

• Project/Area Number: 24300145
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Kurume University
• Principal Investigator: TAKANO Makoto 久留米大学, 医学部, 教授 (30236252)
• Co-Investigator(Kenkyū-buntansha): ITOH Masayuki 久留米大学, 医学部, 助教 (20442535) ; TAKEYA Mitsue 久留米大学, 医学部, 助教 (30289433)
• Co-Investigator(Renkei-kenkyūsha): YAMASHITA Jun 京都大学, iPS細胞研究所, 教授 (50335288) ; KUWAHARA Koichiro 京都大学, 医学研究科, 講師 (30402887)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: 洞房結節 / ペースメーカー細胞 / イオンチャネル / 心臓 / 再プログラミング / 再生心筋 / バイオペースメーカー / HCN4 / 転写因子 / iPS細胞 / 直接再プログラミング / Hcn4

Outline of Final Research Achievements

As a next-generation therapy for bradyarrhythmia, development of biologically engineered pacemaker cells is anticipated. HCN4 channel is widely known as a specific molecular marker for the pacemaker cell of sinoatrial node. In order to visualize HCN4-expressing bio-pacemaker cell, we generated transgenic mouse, in which cDNA of firefly luciferase-GFP fusion protein was knocked in at HCN4 locus (HCN4+/Luc). 15 min after intraperitoneal injection of 50 mg/kg luciferin, we could successfully visualized HCN4-expresssing myocyte in SAN area. Weak but significant luminescence of cultured neonatal ventricular myocyte (NVM) could be also detected using LAS4000 system. However, lentiviral transfection of Tbx3 or Tbx18 into NVM failed to increase HCN4-derived luminescence.

Research Products

• [Journal Article] The hyperpolarization-activated cyclic nucleotide-gated (HCN) channels contain multiple S-palmitoylation sites (2016)
  • Author(s): Itoh, M., Ishihara, K., Nakashima, N., Takano, M.
  • Journal Title: J Physiol Sci Volume: 66 Issue: 3 Pages: 241-8
  • DOI: 10.1007/s12576-015-0420-5
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Clinical Concentrations of Local Anesthetics Bupivacaine and Lidocaine Differentially Inhibit Human Kir2.x Inward Rectifier K+ Channels (2016)
  • Author(s): Nakahira, K., Oshita, K., Itoh, M., Takano, M., Sakaguchi, Y., Ishihara, K.
  • Journal Title: Anesth Analg Volume: 122 Issue: 4 Pages: 1038-47
  • DOI: 10.1213/ane.0000000000001137
  • Peer Reviewed
• [Journal Article] Neuronal and glial expression of inward rectifier potassium channel subunits Kir2.x in rat dorsal root ganglion and spinal cord (2016)
  • Author(s): Murata Y, Yasaka T, Takano M, Ishihara K
  • Journal Title: Neuroscience Letters Volume: 617 Pages: 59-65
  • DOI: 10.1016/j.neulet.2016.02.007
  • Peer Reviewed
• [Journal Article] Renal Nerve-Mediated Erythropoietin Release Confers Cardioprotection During Remote Ischemic Preconditioning (2015)
  • Author(s): Oba T, Yasukawa H, Nagata T, Kyogoku S, Minami T, Nishihara M, Ohshima H, Mawatari K, Nohara S, Takahashi J, Sugi S, Igata S, Iwamoto Y, Kai H, Matsuoka H, Takano M, Aoki H, Fukumoto Y, Imaizumi I.
  • Journal Title: Circulation Journal Volume: 79 Issue: 7 Pages: 1557-1567
  • DOI: 10.1253/circj.CJ-14-1171
  • NAID: 130005083944
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Genotype-dependent differences in age of manifestation and arrhythmia complications in short QT syndrome. (2015)
  • Author(s): Harrell DT, Ashihara T, Ishikawa T, et al.
  • Journal Title: International Journal of Cardiology. Volume: 190 Pages: 393-402
  • DOI: 10.1016/j.ijcard.2015.04.090
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ペースメーカーチャネル(HCN)の病態生理 (2015)
  • Author(s): 鷹野 誠
  • Journal Title: 臨と研 Volume: 92 Pages: 134-137
• [Journal Article] Disturbance of cardiac gene expression and cardiomyocyte structure predisposes Mecp2-null mice to arrhythmias. (2015)
  • Author(s): Hara M, Takahashi T, Mistumasu C, Igata S, Takano M, Minami T, Yasukawa H, Okayama S, Nakamura K, Okabe Y, Tanaka E, Takemura G, Kosai K, Yamashita Y, Matsuishi T.
  • Journal Title: Sci Rep Volume: 5 Issue: 1 Pages: 11204-11204
  • DOI: 10.1038/srep11204
  • Peer Reviewed / Open Access
• [Journal Article] Enhancement of Spontaneous Activity by HCN4 overexpression in Mouse Embryonic Stem Cell-derived Cardiomyocytes - a Possible Biological Pacemaker. (2015)
  • Author(s): SaitoY, Nakamura K, Yoshida M, Sugiyama H, Ohe T, Kurokawa J, Furukawa T, Takano M, Nagase S, Morita H, Kusano KF, Ito H.
  • Journal Title: PLoS ONE Volume: 10 Issue: 9 Pages: e0138193-e0138193
  • DOI: 10.1371/journal.pone.0138193
  • Peer Reviewed / Open Access
• [Journal Article] Ectopic automaticity induced in ventricular myocytes by transgenic overexpression of HCN2. (2015)
  • Author(s): Oshita K, Itoh M, Hirashima S, Kuwabara Y, Ishihara K, Kuwahara K, Nakao K, Kimura T, Nakamura K, Ushijima K, Takano M.
  • Journal Title: Journal of Molecular and Cellular Cardiology Volume: 80 Pages: 81-89
  • DOI: 10.1016/j.yjmcc.2014.12.019
  • NAID: 120007100131
  • Peer Reviewed / Open Access
• [Journal Article] Mechanism of the complete block of the Kir2.1 inward rectifier K+ channel under the external K+-free condition. (2015)
  • Author(s): Yanagi-Ishihara K, Itoh M, Takano M
  • Journal Title: The Journal of Physiological Sciences Volume: 65
• [Journal Article] Zdhhc3/7, the members of protein-palmitoylation enzymes, inhibit the current amplitude of HCN2 channel. (2015)
  • Author(s): Itoh M, Ishihara K, Takano M
  • Journal Title: The Journal of Physiological Sciences Volume: 65
• [Journal Article] Luminescence imaging of HCN4 expression and phenotypic analysis of HCN4 TET-off mouse. (2015)
  • Author(s): Kozasa Y, Ohshita K, Nakashima N, Ushijima K, Takano M
  • Journal Title: The Journal of Physiological Sciences Volume: 65
• [Journal Article] Epithelium-dependent periodical excitation in response to stretch of guinea pig seminal vesicle. (2015)
  • Author(s): Takeya M, Hayashi T, Nakamura K, Takano M
  • Journal Title: The Journal of Physiological Sciences Volume: 65
• [Journal Article] 過分極誘発陽イオンチャネル（HCN2）の潜在的な催不整脈性. (2014)
  • Author(s): 大下健輔, 伊藤政之, 井形幸代, 桒原佳宏, 桑原宏一郎, 牛島一男, 鷹野 誠
  • Journal Title: 日本生理学雑誌 Volume: 76 Pages: 83-83
• [Journal Article] QT短縮症候群に同定されたK チャネル変異の電気生理学的特性と臨床像. (2014)
  • Author(s): ダニエル・トシオ・ハーレル, 芦原貴司, 冨永伊知子, 阿部圭祐, 石川泰輔, 住友直方, 鵜野起久也, 鷹野 誠, 蒔田直昌
  • Journal Title: 日本生理学雑誌 Volume: 76 Pages: 79-79
• [Journal Article] Persistent α1-adrenergic receptor function in the nucleus locus coeruleus causes hyperexcitability in AD/HD model rats (2014)
  • Author(s): Igata S, Hayashi T, Itoh M, Akasu T, Takano M, Ishimatsu M
  • Journal Title: J Neurophysiol Volume: 111 Issue: 4 Pages: 777-786
  • DOI: 10.1152/jn.01103.2012
  • Peer Reviewed
• [Journal Article] Increased expression of HCN channels in the ventricular myocardium contributes to enhanced arrhythmicity in mouse failing hearts. (2013)
  • Author(s): Kuwabara, Y., Kuwahara, K., Takano, M., Kinoshita, H., Arai, Y., Yasuno, S., Nakagawa, Y., Igata, S., Usami, S., Minami, T., Yamada, Y., Nakao, K., Yamada, C., Shibata, J., Nishikimi, T., Ueshima, K., Nakao, K.
  • Journal Title: Journal of the American Heart Association Volume: 2(3) Issue: 3
  • DOI: 10.1161/jaha.113.000150
  • Peer Reviewed
• [Journal Article] Arrhythmogenic coupling between the Na^<+->Ca^<2+> exchanger and inositol 1,4,5-triphosphate receptor in rat pulmonary vein cardiomyocytes (2012)
  • Author(s): Okamoto Y, Takano M, Ohba T, Ono K
  • Journal Title: J Mol Cell Cardiol Volume: 52(5) Issue: 5 Pages: 988-997
  • DOI: 10.1016/j.yjmcc.2012.01.007
  • Peer Reviewed
• [Journal Article] 過分極誘発陽イオンチャネルの発現制御と病態生理 (2012)
  • Author(s): 鷹野誠
  • Journal Title: 久留米医会誌 Volume: 75 Pages: 173-179
• [Journal Article] 肺静脈心筋細胞におけるノルエピネフリン誘発自動能へのCaクロックの関与 (2012)
  • Author(s): 尾野恭一
  • Journal Title: 日生誌 Volume: 74 Pages: 211-213
• [Journal Article] 次世代走査型電子顕微鏡FIB/SEMによる平滑筋組織間質細胞の解析 (2012)
  • Author(s): 中村桂一郎
  • Journal Title: 日生誌 Volume: 74 Pages: 293-294
• [Presentation] HCN4過剰発現・ノックアウトマウスにおける洞房結節機能の変化. (2016)
  • Author(s): 鷹野 誠, 小佐々優子, 武谷三恵, 中島則行, 石原圭子
  • Organizer: 第55回日本生体医工学会大会
  • Place of Presentation: 富山国際会議場(富山）
  • Year and Date: 2016-04-26
• [Presentation] Role of mucosa in generating spontaneous activity in the guinea pig seminal vesicle. (2016)
  • Author(s): Takeya M, Hashitani H, Hayashi T, Nakamura K, Takano M
  • Organizer: The 93rd Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Sapporo Convention Center(札幌）
  • Year and Date: 2016-03-22
• [Presentation] Revisiting K+ dependence of conductance and gating of strong inward rectifier K+ channels. (2016)
  • Author(s): Ishihara K, Itoh M, Igata S, Takano M.
  • Organizer: The 93rd Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Sapporo Convention Center(札幌）
  • Year and Date: 2016-03-22
• [Presentation] he role of pacemaker channel HCN4 against bradycardic response. (2016)
  • Author(s): Kozasa Y, Takeya M, Nakashima N, Ushijima K, Takano M
  • Organizer: The 93rd Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Sapporo Convention Center(札幌）
  • Year and Date: 2016-03-22
• [Presentation] Visualization of HCN4-expressing neurons with GFP using Tet-expression system. (2016)
  • Author(s): Nakashima N, Takano M.
  • Organizer: The 93rd Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Sapporo Convention Center(札幌）
  • Year and Date: 2016-03-22
• [Presentation] Arrhythmogenic properties of rat pulmonary vein cardiomyocytes. (2016)
  • Author(s): Okamoto Y, Takano M, Ono K.
  • Organizer: The 93rd Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Sapporo Convention Center(札幌）
  • Year and Date: 2016-03-22
• [Presentation] ペースメーカーチャネルHCN4の陰性変時作用における新たな機能. (2015)
  • Author(s): 小佐々優子, 武谷三恵, 中島則行, 牛島一男, 鷹野 誠
  • Organizer: 第66回西日本生理学会
  • Place of Presentation: 久留米大学筑水会館(久留米市）
  • Year and Date: 2015-10-09
• [Presentation] ノックインマウスを使ったHCN4 発現ニューロンの可視化. (2015)
  • Author(s): 中島則行, 鷹野 誠
  • Organizer: 第66回西日本生理学会
  • Place of Presentation: 久留米大学筑水会館(久留米市）
  • Year and Date: 2015-10-09
• [Presentation] 内向き整流性カリウムチャネルのポアの開口には細胞外カリウムイオンの存在を必要としない. (2015)
  • Author(s): 柳（石原）圭子, 井形幸代, 伊藤政之, 鷹野 誠
  • Organizer: 平成27年度生理学研究所研究会「膜システムの機能的・構造的統合」
  • Place of Presentation: 生理学研究所(岡崎）
  • Year and Date: 2015-09-01
• [Presentation] モルモット精嚢平滑筋自動性における粘膜の役割. (2015)
  • Author(s): 武谷三恵, 橋谷 光, 林 篤正, 中村桂一郎, 鷹野 誠
  • Organizer: 第57回日本平滑筋学会総会
  • Place of Presentation: 山口大学小串キャンパス（医学部）総合研究棟(山口）
  • Year and Date: 2015-08-25
• [Presentation] Controversy still goes on; lessons from HCN4 knock-out mice. (2015)
  • Author(s): Takano M, Kozasa Y, Takeya M, Ohshita K, Ito M, Ishihara K, Nakashima N.
  • Organizer: The Joint Meeting of the 30th Annual Meeting of the Japanese Heart Rhythm Society and the 32nd Annual Scientific Meeting of the Japanese Society of Electrocardiology
  • Place of Presentation: Kyoto International Conference Center(京都）
  • Year and Date: 2015-07-28
• [Presentation] Atrial fibrillation and arrhythmogenic activities of pulmonary vein cardiomyocytes. (2015)
  • Author(s): Ono K, Okamoto Y, Umehara S, Adachi T, Ohba T, Amano A, Takano M.
  • Organizer: The Joint Meeting of the 30th Annual Meeting of the Japanese Heart Rhythm Society and the 32nd Annual Scientific Meeting of the Japanese Society of Electrocardiology
  • Place of Presentation: Kyoto International Conference Center(京都）
  • Year and Date: 2015-07-28
• [Presentation] Priori Silvia G, Makita N: Meta-analysis of short QT syndrome discloses genotype-dependent clinical characteristics in age of manifestation and arrhythmia complications. (2015)
  • Author(s): Harrell Daniel T, Ashihara T, Ishikawa T, Mazzanti A, Takahashi K, Oginosawa Y, Abe H, Maemura K, Sumitomo N, Uno K, Takano M, Priori Silvia G, Makita N.
  • Organizer: The Joint Meeting of the 30th Annual Meeting of the Japanese Heart Rhythm Society and the 32nd Annual Scientific Meeting of the Japanese Society of Electrocardiology
  • Place of Presentation: Kyoto International Conference Center(京都）
  • Year and Date: 2015-07-28
• [Presentation] Luminescence imaging of HCN4 expression and phenotypic analysis of HCN4 TET-off mouse. (2015)
  • Author(s): Kozasa Y, Ohshita K, Nakashima N, Ushijima K, Takano M.
  • Organizer: The 92nd Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 神戸コンベンションセンター(兵庫県神戸市）
  • Year and Date: 2015-03-22
• [Presentation] Epithelium-dependent periodical excitation in response to stretch of guinea pig seminal vesicle. (2015)
  • Author(s): Takeya M, Hayashi T, Nakamura K, Takano M.
  • Organizer: The 92nd Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 神戸コンベンションセンター(兵庫県神戸市）
  • Year and Date: 2015-03-22
• [Presentation] Mechanism of the complete block of the Kir2.1 inward rectifier K+ channel under the external K+-free condition. (2015)
  • Author(s): Yanagi-Ishihara K, Itoh M, Takano M.
  • Organizer: The 92nd Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 神戸コンベンションセンター(兵庫県神戸市）
  • Year and Date: 2015-03-21
• [Presentation] Zdhhc3/7, the members of protein-palmitoylation enzymes, inhibit the current amplitude of HCN2 channel. (2015)
  • Author(s): Itoh M, Ishihara K, Takano M.
  • Organizer: The 92nd Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 神戸コンベンションセンター(兵庫県神戸市）
  • Year and Date: 2015-03-21
• [Presentation] 化学発光によるHCN4発言部位の可視化. (2014)
  • Author(s): 小佐々優子
  • Organizer: 第65回西日本生理学会
  • Place of Presentation: 琉球大学(沖縄県中頭郡西原町）
  • Year and Date: 2014-10-24
• [Presentation] Differential expression of Kir2.x inward rectifier K+ channels in neurons and glial cells in rat dorsal root ganglion and spinal cord. (2014)
  • Author(s): Murata Y, Honda Y, Yasaka T, Takano M, Masuko S, Ishihara K.
  • Organizer: The 37th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市）
  • Year and Date: 2014-09-11
• [Presentation] Analysis of cardiac arrhythmias and gene expression in MeCP2-null Mouse. (2014)
  • Author(s): Hara M, Takahashi T, Mitsumasu C, Igata S, Takano M, Okabe Y, Tanaka E, Matsuishi T.
  • Organizer: 13th Rett Syndrome Symposium
  • Place of Presentation: Chantilliy, VA, USA
  • Year and Date: 2014-06-24 – 2014-06-26
• [Presentation] Functional consequences of a gain-of-function mutation in the Kir2.1 inward rectifier K+ channel associated with short QT syndrome (2014)
  • Author(s): Ishihara K, Itoh M, Igata S, Takano M
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島郡元キャンパス（鹿児島）
• [Presentation] Identification of palmitoylation enzymes for the HCN2 channel (2014)
  • Author(s): Itoh M, Takano M
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島）
• [Presentation] Induced automaticity in ventricular myocytes from transgenic mice overexpressing HCN2 (2014)
  • Author(s): Oshita K, Itoh M, Yanagi(Ishihara) K, Kuwabara Y, Kuwahara K, Ushijima K, Takano M
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島）
• [Presentation] Epithelium of seminal vesicle modulates neural activity dependent contraction and originates stretch-sensitive contraction (2014)
  • Author(s): Takeya M, Itoh M, Ishihara K, Hayashi T, Nakamura K, Takano M
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島）
• [Presentation] Pathophysiological roles of HCN channel family in the heart (2014)
  • Author(s): Takano M, Oshita K, Itoh M, Ishihara K, Kuwahara K
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島）
• [Presentation] Arrhythmogenic nature of pulmonary vein cardiomyocyes. (2013)
  • Author(s): 尾野恭一
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船橋(東京都)
  • Year and Date: 2013-03-29
• [Presentation] Clinical manifestations and electrophysiological characteristics of K channel mutations responsible for short QT syndrome. (2013)
  • Author(s): Harrell D
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船橋(東京都)
  • Year and Date: 2013-03-29
• [Presentation] Epithelium of seminal vesicle confers stretch sensitivity and modulates noradrenaline-induced contraction. (2013)
  • Author(s): 武谷三恵
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船橋(東京都)
  • Year and Date: 2013-03-29
• [Presentation] Isoproterenol-induced spontaneous action potentials in the cardiac myocytes of transgenic mouse overexpressing HCN2. (2013)
  • Author(s): 大下健輔
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船橋(東京都)
  • Year and Date: 2013-03-27
• [Presentation] Stromal cells play important roles in tissue compartmentalization: A FIB/SEM study. (2013)
  • Author(s): 中村桂一郎
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船橋(東京都)
  • Year and Date: 2013-03-27
• [Presentation] Identification of the palmitoylation sites of HCN2 channel. (2013)
  • Author(s): 伊藤政之
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船橋(東京都)
  • Year and Date: 2013-03-27
• [Presentation] HCN2過剰発現マウス心室筋のイソプロテレノール誘発自動能 (2013)
  • Author(s): 大下健輔, 井形幸代, 桑原佳宏, 桑原宏一郎, 鷹野 誠, 牛島一男
  • Organizer: 日本麻酔科学会第60回学術集会
  • Place of Presentation: 札幌市教育文化会館（札幌）
• [Presentation] Induced automaticity in the ventricular myocytes of transgenic mouse overexpressing HCN2 (2013)
  • Author(s): Oshita K, Igata S, Kuwabara Y, Kuwahara K, Ushijima K, Takano M
  • Organizer: IUPS 2013
  • Place of Presentation: International Convention Center(Birmingham, UK）
• [Presentation] QT短縮症候群患者に同定された新規K+チャネル変異の電気生理学的特性と臨床像 (2013)
  • Author(s): ダニエル ハーレル, 冨永伊知子, 安部圭祐, 渡部太一, 荻ノ沢泰司, 安部治彦, 住友直方, 鵜野起久也, 鷹野 誠, 蒔田直昌
  • Organizer: 第30回日本心電学会学術集会
  • Place of Presentation: リンクステーションホール青森(青森）
• [Presentation] Lasting activity of alpha1-AR during postnatal development alters membrane properties of LC in SHR (2013)
  • Author(s): Igata S, Hayashi T, Itoh M, Takano M, Ishimatsu M
  • Organizer: Society for Neuroscience, 43rd Annual Meeting (Neuroscience 2013)
  • Place of Presentation: San Diego Convention Center(San Diego, USA)
• [Presentation] S-palmitoylation regulates the HCN channels. (2012)
  • Author(s): Itoh M
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: マリンメッセ福岡(福岡市)
  • Year and Date: 2012-12-15
• [Presentation] HCN2を心臓特異的に発現する遺伝子改変マウス心室筋のイソプロテレノール誘発自動能 (2012)
  • Author(s): 大下健輔
  • Organizer: 平成24年度生理学研究所研究会「イオンチャネル・トランスポーターと心血管機能:細胞機能の分子機序とその統合的理解」
  • Place of Presentation: 生理学研究所(岡崎市)
  • Year and Date: 2012-11-22
• [Presentation] モルモット精嚢の収縮特性に対する内膜の影響 (2012)
  • Author(s): 武谷三恵
  • Organizer: 第63回西日本生理学会
  • Place of Presentation: 全労済ソレイユ(大分市)
  • Year and Date: 2012-10-20
• [Presentation] HCN2過剰発現マウス心室筋のイソプロテレノール誘発自動能 (2012)
  • Author(s): 大下健輔
  • Organizer: 第63回西日本生理学会
  • Place of Presentation: 全労済ソレイユ(大分市)
  • Year and Date: 2012-10-19
• [Presentation] パルミトイル化によるHCNチャネルの機能制御 (2012)
  • Author(s): 伊藤政之
  • Organizer: 第63回西日本生理学会
  • Place of Presentation: 全労済ソレイユ(大分市)
  • Year and Date: 2012-10-19
• [Presentation] An α1 adrenergic receptor (AR) stimulation compensates α2-AR induced outward current in locus coeru'eus neurons from spontaneous hypertensive rats, ADHD model animal. (2012)
  • Author(s): Igata S
  • Organizer: Society for Neuroscience, 42nd Annual Meeting (Neuroscience 2012)
  • Place of Presentation: Emest N. Morial Convention Center (New Orleans, USA)
  • Year and Date: 2012-10-15
• [Presentation] 過分極誘発陽イオンチャネル（HCN2)の潜在的な催不整脈性
  • Author(s): 大下健輔, 伊藤政之, 井形幸代, 桑原佳宏, 桑原宏一郎, 牛島一男, 鷹野 誠
  • Organizer: 第23回日本循環薬理学会
  • Place of Presentation: 福岡大学メディカルホール（福岡）
• [Book] 不整脈学 (2012)
  • Author(s): 鷹野誠
  • Publisher: 南江堂
• [Remarks] 久留米大学研究者紹介
  • URL: http://research.kurume-u.ac.jp/data.php?scode=14905632890988
• [Remarks] 久留米大学研究者紹介
  • URL: http://research.kurume-u.ac.jp/data.php?scode=76735632890988
• [Remarks] 久留米大学医学部生理学講座統合自失機能部門研究業績
  • URL: http://www.med.kurume-u.ac.jp/med/physiol2/paper.html
• [Remarks] 久留米大学研究者紹介
  • URL: http://research.kurume-u.ac.jp/data.php?scode=73005632890988
• [Remarks] 久留米大学医学部生理学講座統合自律機能部門研究業績
  • URL: http://www.med.kurume-u.ac.jp/med/physiol2/paper.html
• [Remarks]
  • URL: http://www.med.kurume-u.ac.jp/med/physiol2/paper.html