Development of autologous scaffolds

• Project/Area Number: 24300177
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: National Institute for Materials Science
• Principal Investigator: CHEN Guoping 独立行政法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, ユニット長 (50357505)
• Co-Investigator(Renkei-kenkyūsha): KAWAZOE Naoki 物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, MANA研究者 (90314848)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: マトリックス / 足場材料 / 三次元細胞培養 / 鋳型法 / 脱細胞化 / 多孔質構造 / 生体親和性

Outline of Final Research Achievements

A method to prepare cultured cells derived extracellular matrix scaffolds was developed by using three-dimensional cell culture and template method. Various cell types can be used for preparation of their respective extracellular matrix scaffolds. Autologous extracellular matrix scaffolds prepared from autologous cells showed excellent biocompatibility without eliciting adverse inflammatory and immune responses when being implanted. The scaffolds will be useful for control the functions of stem cells and for regenerative medicine.

Research Products

• [Journal Article] Influence of surfaces modified with biomimetic extracellular matrices on adhesion and proliferation of mesenchymal stem cells and osteosarcoma cells (2015)
  • Author(s): Rong Cai, Naoki Kawazoe and Guoping Chen
  • Journal Title: Colloids and Surfaces B: Biointerfaces Volume: 126 Pages: 381-386
  • DOI: 10.1016/j.colsurfb.2014.11.050
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Influence of stepwise chondrogenesis-mimicking 3D extracellular matrix on chondrogenic differentiation of mesenchymal stem cells (2015)
  • Author(s): Rong Cai, Tomoko Nakamoto, Naoki Kawazoe and Guoping Chen
  • Journal Title: Biomaterials Volume: 52 Pages: 199-207
  • DOI: 10.1016/j.biomaterials.2015.02.033
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Stem cell culture using cell-derived substrates (2014)
  • Author(s): Binata Joddar, Takashi Hoshiba, Guoping Chen, and Yoshihiro Ito
  • Journal Title: Biomaterials Science Volume: 2 Issue: 11 Pages: 1595-1603
  • DOI: 10.1039/c4bm00126e
  • Peer Reviewed
• [Journal Article] Effects of extracellular matrix proteins in chondrocyte-derived matrices on chondrocyte functions (2013)
  • Author(s): Takashi Hoshiba, Tomoe Yamada, Hongxu Lu, Naoki Kawazoe, Guoping Chen
  • Journal Title: Biotechnology Progress Volume: 29 Issue: 5 Pages: 1331-1336
  • DOI: 10.1002/btpr.1780
  • Peer Reviewed
• [Journal Article] Preparation of Collagen Scaffolds with Controlled Pore Structures and Improved Mechanical Property for Cartilage Tissue Engineering (2013)
  • Author(s): Qin Zhang, Hongxu Lu, Naoki Kawazoe, Guoping Chen
  • Journal Title: Journal of Bioactive and Compatible Polymers Volume: 28(in press) Issue: 5 Pages: 426-438
  • DOI: 10.1177/0883911513494620
  • Peer Reviewed
• [Journal Article] Comparison of Decellularization Techniques for Preparation of Extracellular Matrix (2012)
  • Author(s): Hongxu Lu, Takashi Hoshiba, Naoki Kawazoe, Guoping Chen
  • Journal Title: Journal of Biomedical Materials Research : PartA Volume: 100 Issue: 9 Pages: 2507-2516
  • DOI: 10.1002/jbm.a.34150
  • Peer Reviewed
• [Presentation] Design and Preparation of Porous Scaffolds and Biomimetic Matrices for Tissue Engineering (2014)
  • Author(s): Guoping Chen
  • Organizer: 1st Asian University Symposium on Biomedical Engineering
  • Place of Presentation: 台湾
  • Year and Date: 2014-12-12 – 2014-12-14
  • Invited
• [Presentation] Development of Biomimetic 3D ECM Scaffolds for Tissue Regeneration (2014)
  • Author(s): Chen G, Cai R, Nakamoto T, Kawazoe N
  • Organizer: 7th World Congress on Preventive and Regenerative Medicine
  • Place of Presentation: 台湾
  • Year and Date: 2014-11-04 – 2014-11-07
  • Invited
• [Presentation] Effects of Stepwise Tissue Development-Mimicking Matrices on MSCs Differentiation (2014)
  • Author(s): Guoping Chen, Rong Cai, Takashi Hoshiba and Naoki Kawazoe
  • Organizer: 2014 International Symposium of Materials on Regenerative Medicine
  • Place of Presentation: 台湾
  • Year and Date: 2014-08-27 – 2014-08-29
  • Invited
• [Presentation] Preparation of Functional Polymer Scaffolds for Tissue Engineering (2014)
  • Author(s): Guoping Chen, Qin Zhang, Hongxu Lu and Naoki Kawazoe
  • Organizer: NIMS Conference 2014
  • Place of Presentation: つくば
  • Year and Date: 2014-07-01 – 2014-07-03
• [Presentation] Hybrid Polymer and Biomimetic Matrix Scaffolds for Tissue Engineering (2014)
  • Author(s): Guoping Chen
  • Organizer: 2014 International Symposium on Nano-biomaterials and Nanomedicine
  • Place of Presentation: 香港
  • Year and Date: 2014-04-28
  • Invited
• [Presentation] 骨及び脂肪組織発生模倣型マトリックスの作製 (2014)
  • Author(s): 陳国平, 干場隆志, 川添直輝
  • Organizer: 第13回日本再生医療学会総会
  • Place of Presentation: 京都
• [Presentation] Biomimetic matrices and hybrid scaffolds for tissue engineering (2013)
  • Author(s): Guoping Chen, Hongxu Lu, Takashi Hoshiba, Hwan Hee Oh, Naoki Kawazoe
  • Organizer: 22nd Annual Meeting of Australasian Society for Biomaterials and Tissue Engineering
  • Place of Presentation: Barossa Valley, Australia
  • Invited
• [Presentation] Biomimetic Matrices and Porous Scaffolds for Tissue Engineering (2013)
  • Author(s): Guoping Chen
  • Organizer: 2013 International Conference on Applied Biomaterials
  • Place of Presentation: Taiwan
  • Invited
• [Presentation] Extracellular Matrix-Based Biomaterials for Tissue Engineering (2013)
  • Author(s): Guoping Chen, Hongxu Lu, Takashi Hoshiba, Naoki Kawazoe
  • Organizer: 7th International Conference on the Science and Technology for Advanced Ceramics
  • Place of Presentation: 横浜
  • Invited
• [Presentation] Cultured cell-derived extracellular matrix scaffolds for (2013)
  • Author(s): Guoping Chen, Hongxu Lu, Takashi Hoshiba, Naoki Kawazoe
  • Organizer: 4th Asian Biomaterials Congress
  • Place of Presentation: Hong Kong
  • Invited
• [Presentation] 生体組織発生模倣型マトリックスの創出及び幹細胞分化制御への応用 (2013)
  • Author(s): 陳国平, CAIRong, 干場隆志, 川添直輝
  • Organizer: 第62回高分子討論会
  • Place of Presentation: 金沢
• [Presentation] Design and Fabrication of Biodegradable Polymer Porous Scaffolds and Biomimetic Matrices for Tissue Engineering (2013)
  • Author(s): Guoping Chen
  • Organizer: 2013 Symposium on Biomaterials for Regenerative Medicine
  • Place of Presentation: Shanghai, China
  • Invited
• [Presentation] Biomimetic ECM and Polymeric Porous Scaffolds for Tissue Engineering (2013)
  • Author(s): Guoping Chen
  • Organizer: 中国生物材料学会2013年大会
  • Place of Presentation: Shenzhen, China
  • Invited
• [Presentation] 培養細胞由来のマトリックス足場材料の作製 (2012)
  • Author(s): 陳国平、呂宏旭、川添直輝
  • Organizer: 日本バイオマテリアル学会シンポジウム2012
  • Place of Presentation: 仙台国際センター(仙台)
  • Year and Date: 2012-11-27
• [Presentation] ECM Scaffolds Prepared from Cultured Cells (2012)
  • Author(s): Guoping Chen, Hongxu Lu, and Naoki Kawazoe
  • Organizer: 12^<th> Asia BioCeramics Symposium
  • Place of Presentation: 成功大学(台湾)(招待講演)
  • Year and Date: 2012-11-20
• [Presentation] Biomimetic Matrices and Scaffolds Derived from Cultured Cells (2012)
  • Author(s): Guoping Chen, Hongxu Lu, Takashi Hoshiba, Naoki Kawazoe
  • Organizer: IUMRS-International Conference on Electronic Materials(IUMRS.ICEM 2012)
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2012-09-27
• [Presentation] Autologous Extracellular Matrix Scaffolds Prepared from Cultured Cells (2012)
  • Author(s): Guoping Chen, Hongxu Lu, and Naoki Kawazoe
  • Organizer: 3^<rd> TERMIS World Congress
  • Place of Presentation: Hofburg Congress Centre (Vienna, Austria)
  • Year and Date: 2012-09-06
• [Presentation] Cultured Cell-Derived Matrices for Tissue Engineering and Stem Cell Function (2012)
  • Author(s): Guoping Chen, Hongxu Lu, Takashi Hoshiba, Naoki Kawazoe
  • Organizer: 2012 International Symposium of Materials on Regenerative Medicine (2012 ISOMRM)
  • Place of Presentation: Taipei Medical University (Taipei, Taiwan)
  • Year and Date: 2012-08-29
• [Presentation] Development of Biomimetic Autologous Extracellular Matrix scaffolds (2012)
  • Author(s): Hongxu Lu, Takashi Hoshiba, Naoki Kawazoe, Guoping Chen
  • Organizer: 9^<th> World Biomaterials Congress
  • Place of Presentation: New Century International Exhibition and Convention Center (Chengdu, China)
  • Year and Date: 2012-06-04
• [Presentation] Biomimetic Extracellular Matrix Biomaterials for Stem Cell Function Manipulation and Tissue Regeneration (2012)
  • Author(s): Guoping Chen, Hongxu Lu, Takashi Hoshiba, Naoki Kawazoe
  • Organizer: International Forum of Biomedical Materials : Nanobiomaterials for Tissue Regeneration
  • Place of Presentation: Zhejiang University (Hangzhou, China)(招待講演)
  • Year and Date: 2012-05-30
• [Presentation] 細胞由来のECM足場材料の軟骨組織再生への応用 (2012)
  • Author(s): 陳国平、呂宏旭、川添直輝
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2012-03-22
• [Book] 動物細胞培養の手法と細胞死・増殖不良・細胞変異を防止する技術 (2014)
  • Author(s): 陳国平, 川添直輝
  • Total Pages: 5
  • Publisher: 技術情報協会
• [Book] Biomimetics: Advancing Nanobiomaterials and Tissue Engineering (2013)
  • Author(s): Guoping Chen, Hongxu Lu, Naoki Kawazoe
  • Total Pages: 9
  • Publisher: Scrivener Publishing, Wiley
• [Book] World Scientific (2013)
  • Author(s): Guoping Chen, Takashi Hoshiba, Naoki Kawazoe
  • Total Pages: 13
  • Publisher: Tissue Regeneration: Where Nano-Structures Meets Biology
• [Book] 体内埋め込み医療材料の開発とその理想的な性能・デザインの要件 (2013)
  • Author(s): 陳国平、川添直輝
  • Total Pages: 4
  • Publisher: 技術情報協会