Exploring food-derived factors that affect skeletal muscle metabolism for prevention of chronic diseases
Project/Area Number |
24300254
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Eating habits, studies on eating habits
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Research Institution | Ochanomizu University |
Principal Investigator |
Iida Kaoruko お茶の水女子大学, 基幹研究院, 准教授 (50375458)
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Co-Investigator(Kenkyū-buntansha) |
KONDO Kazuo 東洋大学, 食環境学部, 教授 (30153711)
SUZUKI Emiko お茶の水女子大学, 基幹研究院, 教授 (80154524)
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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Keywords | 骨格筋 / 食品因子 / 肥満 / ミトコンドリア / エネルギー代謝 / 慢性炎症 / 脂質代謝 / 大豆イソフラボン / 栄養代謝 / 転写因子 / 遺伝子発現 / 炎症反応 / 低糖質食 / 骨格筋細胞 |
Outline of Final Research Achievements |
This study aimed to identify food-derived factors that affect muscle metabolism in obesity. For this purpose, we used the C2C12 murine muscle cell line. Screening based on the promoter assay suggested that soy isoflavone daidzein was a potent candidate that regulated mitochondrial biogenesis. Treatment of C2C12 myotubes with daidzein resulted in significant increases in the expression of mitochondrial transcription factor A (Tfam) as well as cytochrome b and COX1 as its targets. We found that the nuclear respiratory factor (NRF) were necessary for the effect of daidzein on Tfam expression. In addition, daidzein regulated cytokine expression through the PPAR-α and -γ in animal adipose tissue and cultured adipocytes, thereby improving the adverse effects of adipose inflammation in obesity. We also examined the effect of ketogenic nutrient environment on muscle metabolism in mice and found that ketotic states promote transcriptional regulation of various metabolic genes in muscles.
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Report
(5 results)
Research Products
(32 results)
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[Presentation] 脂質摂取と2型糖尿病リスクとの関連性についてのメタ解析
Author(s)
田島諒子, 児玉暁, 堀川千嘉, 藤原和哉, 吉澤咲子, 平安座依子, 菅原歩美, 谷内洋子, 齋藤あき, 飯田薫子, 曽根博仁
Organizer
第67回 日本栄養・食糧学会大会
Place of Presentation
名古屋大学
Related Report
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