| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2012: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Outline of Final Research Achievements |
DNA double strand break (DSB), which is often induced by ionizing radiation, is one of the most serious DNA damage. To clarify the relationship between DSB repair pathway and mutation induction, we developed a site-specific DSB induction system by using artificial nucleases. We analyzed DSB repair efficiency and mutation spectrum at the DSB site, and found that efficiency and accuracy of DSB repair could be separately regulated. We also found that mutation at FHA domain of NBS1 renders the cells to be sensitive to radiation without increase in mutation frequency. A part of our findings were reported as several papers in scientific journals.
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