Development of new technology using ion beam for the investigation of pathogenesis and an early diagnosis of asbestosis

• Project/Area Number: 24310067
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Quantum beam science
• Research Institution: Gunma University
• Principal Investigator: DOBASHI Kunio 群馬大学, 保健学研究科, 教授 (00241894)
• Co-Investigator(Kenkyū-buntansha): SATO Takahiro 独立行政法人日本原子力開発機構, 放射線高度利用施設部, 研究員 (10370404) ; ISHIZUKA Tamotsu 福井大学, 医学部, 教授 (50302477) ; SHIMIZU Yasuo 獨協医科大学, 医学部, 講師 (80396621)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2012: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: 大気microPIXE / 石綿肺 / アスベスト / 肺線維症 / 特発性間質性肺炎 / 粉塵 / 元素分布 / イオンビーム / 大気マイクロPIXE / 組織内元素分布 / 職業性呼吸器疾患 / 労働災害 / 職業性粉塵暴露 / 肺組織中の元素分布 / 塵肺

Outline of Final Research Achievements

In-air microparticle-induced X-ray emission (in-air micro-PIXE) analysis is employed to assess the spatial distribution and content of small amount of element in lung biopsy specimens. We analyzed the pathogenesis of lung fibrosis induced by inhaled asbestos or dust by microPIXE. The analysis of lung tissues from mice administrated silica particle and lung fibrosis patients showed significant increase of iron level on silica particle. And the expression of markers of oxidative stress and apoptosis were co-localized with silica particles. Lung tissues from 12 lung fibrosis patients were subjected to in-air micro-PIXE analysis. Two IIP patients showed a large quantity of Si and the co-localization of Si and Fe deposition with Fas expression.

Research Products

• [Journal Article] Co-localization of iron binding on silica with p62/sequestosome1 (SQSTM1) in lung granulomas of mice with acute silicosis (2015)
  • Author(s): Shimizu Y, Dobashi K, Nagase H, Ohta K, Sano T et al
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 56 Issue: 1 Pages: 74-83
  • DOI: 10.3164/jcbn.14-44
  • NAID: 130004704980
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] ROCK activation in lung of idiopathic pulmonary fibrosis with oxidative stress (2014)
  • Author(s): Shimizu Y, Dobashi K, Sano T, Yamada M.
  • Journal Title: International journal of immunopathology and pharmacology Volume: 27 Pages: 37-44
  • Peer Reviewed
• [Journal Article] Proton ion-microbeam elemental analysis for inhaled particle-induced pulmonary diseases : application for diagnosis and assessment of progression. (2013)
  • Author(s): Shimizu Y, Dobashi K.
  • Journal Title: Curr Med Chem Volume: 20 Issue: 6 Pages: 789-93
  • DOI: 10.2174/0929867311320060005
  • Peer Reviewed
• [Presentation] In-air microparticle induced X-ray emission analysis of inhaled particulates in thoracic lymph node in a patient with asbestos exposure (2015)
  • Author(s): Yasuhiko Koga
  • Organizer: 2015 ATS International Conference
  • Place of Presentation: Denver, U.S.A.
  • Year and Date: 2015-05-18
• [Presentation] The efficacy of in-air microanalysis of particles in interstitial pneumonia lung tissue (2014)
  • Author(s): 古賀 康彦
  • Organizer: 第９回高崎量子応用研究シンポジウム
  • Place of Presentation: 群馬県高崎市 高崎シティーギャラリー
  • Year and Date: 2014-10-10
• [Presentation] 間質性肺炎症例におけるパラフィン切片を用いたIn-air micro PIXE解析による肺内微粒子の元素解析 (2014)
  • Author(s): 古賀康彦
  • Organizer: 日本職業・環境アレルギー学会
  • Place of Presentation: 福岡ソフトリサーチパーク 福岡市
  • Year and Date: 2014-06-28
• [Presentation] The Efficacy Of Inair Microanalysis Of Particles In Interstitial Pneumonia Lung Tissue In Patients With Or Without Occupational Exposure, (2014)
  • Author(s): Yasuhiko Koga
  • Organizer: 2014 ATS International Conference
  • Place of Presentation: San Diego, USA
  • Year and Date: 2014-05-19
• [Presentation] Analysis on Co-Localization of Si and Fe in Lung Tissue of Silica-Inhaled Mouse by In-Air Micro-PIXE, (2013)
  • Author(s): K.Dobashi
  • Organizer: 2013 ATS International Conference
  • Place of Presentation: Philadelphia, Pennsylvania USA
• [Presentation] 特発性間質性肺炎患者における吸入粉塵の影響の量子ビームによる解析 (2013)
  • Author(s): 土橋邦生
  • Organizer: 第52回日本呼吸器学会学術講演会
  • Place of Presentation: 神戸国際会議場
• [Presentation] 大気マイクロピクシーで診断したアルミニウム肺の一例 (2013)
  • Author(s): 杉崎 緑、
  • Organizer: 第207回日本呼吸器学会関東地方会
  • Place of Presentation: エーザイ株式会社 本社
• [Presentation] ミドリゾウリムシ内のEuの三次元分布測定 (2012)
  • Author(s): 佐藤隆博
  • Organizer: 第28回PIXEシンポジウム
  • Place of Presentation: 東京工業大学大岡山キャンパス
  • Year and Date: 2012-11-09
• [Presentation] 二次性肺胞蛋白症の肺組織切片中の粉塵及びマクロファージ細胞内におけるin-air micro-PIXE解析 (2012)
  • Author(s): 清水泰生
  • Organizer: 第28回PIXEシンポジウム
  • Place of Presentation: 東京工業大学大岡山キャンパス
  • Year and Date: 2012-11-09
• [Presentation] 特発性間質性肺炎患者における吸入粉塵の影響の量子ビームによる解析 (2012)
  • Author(s): 土橋邦生
  • Organizer: 第52回日本呼吸器学会
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2012-04-21
• [Remarks] 国立大学法人群馬大学大学院保健学研究科 土橋研究室
  • URL: http://dobashi-lab.health.gunma-u.ac.jp/21.html
• [Remarks] Micro-PIXEによる石綿肺、吸入粉塵による間質性肺炎の新診断法と病態解明への応用
  • URL: http://dobashi-lab.health.gunma-u.ac.jp/pdf/puxe.pdf