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Creation of molecular recognition tool against biological toxins and development of effective decontamination techniques

Research Project

Project/Area Number 24310125
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Social systems engineering/Safety system
Research InstitutionNational Research Institute of Police Science

Principal Investigator

Seto Yasuo  科学警察研究所, 法科学第三部, 部長 (10154668)

Co-Investigator(Kenkyū-buntansha) UZAWA Hirotaka  国立研究開発法人産業技術総合研究所, ナノシステム研究部門, 上級主任研究員 (60356566)
OHSAWA Isaku  科学警察研究所, 法科学第三部, 主任研究官 (30370886)
MIYAGUCHI Hajime  科学警察研究所, 附属鑑定所, 鑑定官 (10370884)
柘 浩一郎  科学警察研究所, 法科学第三部, 主任研究官 (90356204)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Keywordsテロ対策 / 分子認識 / 機器分析 / 生物毒素 / 吸着
Outline of Final Research Achievements

As evaluation method for molecular recognition tool against biological toxins, surface plasmon resonance (SPR) analytical method and electrospray ionization spectrometry have been developed. As for preparation of candidate molecular recognition tool, artificial antibody against Staphylococcal enterotoxin B (SEB) has been created using phage display method, and also lactose derivatives which reduction terminal are combined with triethylene glycol azide has been synthesized. The most suitable candidate of the commercial antibody preparation against SEB has been selected by measurement of binding affinity and capacity using SPR. Lactose derivatives have been immobilized into monolithic silica by click chemistry-mediated sugar introduction method. The prepared sugar-immobilized monolithic silica tip has been verified for decontamination power capturing ricin.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (4 results)

All 2015 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (1 results)

  • [Journal Article] バイオテロ対策としての微生物現場制御2015

    • Author(s)
      瀬戸康雄
    • Journal Title

      日本防菌黴学会誌

      Volume: 43 Pages: 191-200

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] バイオテロ対処としての微生物現場制御2015

    • Author(s)
      瀬戸康雄
    • Journal Title

      日本防菌防黴学会誌

      Volume: 43 Pages: 191-200

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Glycotechnology for decontamination of biological agents : A model study using ricin and biotin-tagged synthetic glycopolymers2012

    • Author(s)
      T. Nagatsuka, H. Uzawa, K. Sato, I. Ohsawa。Y. Seto and Y. Nishida
    • Journal Title

      ACS Applied Materials & Interfaces

      Volume: 4 Issue: 2 Pages: 832-837

    • DOI

      10.1021/am201493q

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] DEVELOPMENT OF ON-SITE DECONTAMINATION TECHNOLOGIES – PHOTOCATALYTIC DEGRADATION FOR CHEMICAL WARFARE AGENTS AND MOLECULAR RECOGNITION ADSORPTION FOR BIOLOGICAL TOXINS2013

    • Author(s)
      Yasuo Seto, Takeshi Ohmori, Mieko Kanamori-Kataoka, Kouichiro Tsuge, Isaac Ohsawa, Shintaro Kishi, Yuji Urushibata, Keita Sato, Asuka Komano, Hirotaka Uzawa, Takehiro Nagatsuka, Koji Takeuchi, Nobuaki Negishi, Tsutomu Hirakawa and Masahiro Furuno
    • Organizer
      11th International symposium on Protection against Chemical and Biological Warfare agents
    • Place of Presentation
      Stockholm
    • Related Report
      2013 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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