| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Outline of Final Research Achievements |
Prenylated coumarins widely occur in Apiaceae and Rutaceae. They show toxicity to herbivores and pathogens serving as protectants for plants that produce those metabolites, while those coumarin compounds show a variety of biological activities to humans, as well. Prenyltransferase, a key enzyme in the biosynthesis, has been first identified at the gene level from parsley (Apiaceae) and lemon (Rutaceae).
Prenyltransferase family for coumarins show high hydrophobicity via typically nine transmembrane alpha-helices, and they show strict specificity to both prenyl donor and acceptor substrates. For the catalytic function, Mg2+ is required and the subcellular localization of these membrane proteins is demonstrated to be plastids in plant cells.
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