• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Search for bioactive natural products acting at infected region of M. tuberculosis and analysis of their target molecules

Research Project

Project/Area Number 24310159
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Living organism molecular science
Research InstitutionOsaka University

Principal Investigator

Arai Masayoshi  大阪大学, 薬学研究科(研究院), 准教授 (80311231)

Co-Investigator(Renkei-kenkyūsha) KOBAYASHI Motomasa  大阪大学, 大学院薬学研究科, 教授 (40116033)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords抗菌物質 / 結核 / 感染症 / 活性天然物 / ケミカルバイオロジー / 抗生物質
Outline of Final Research Achievements

In order to search for new anti-microbial substances against the dormant Mycobacterium tuberculosis, which shows tolerance to the conventional anti-Tuberculosis drugs, we constructed several screening systems focusing on the characteristic phenotypes of mycobacteria at the infected region. On the guidance of the screening from the extract library of marine sponges and marine-derived microbes, we could explore several anti-microbial compounds including structurally new compounds, which are expected to be a new medicinal seed. In addition, we could clarify the target molecules of some isolated anti-microbial compounds by the method utilizing the transformants overexpressing genomic DNA library, the analysis of gene mutation and/or the pull-down assay using a synthetic probe of the active compound. The clarified target proteins are expected to become a new drug target against the dormant M. tuberculosis.

Report

(4 results)
  • 2015 Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (30 results)

All 2015 2014 2013 2012 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Acknowledgement Compliant: 2 results) Presentation (17 results) (of which Int'l Joint Research: 1 results,  Invited: 5 results) Book (1 results) Remarks (3 results)

  • [Int'l Joint Research] Lampung University(インドネシア)

    • Related Report
      2014 Annual Research Report
  • [Int'l Joint Research] University of KwaZulu Natal(南アフリカ)

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Anti-dormant mycobacterial activity and target analysis of nybomycin produced by a marine-derived Streptomyces sp.2015

    • Author(s)
      Arai, M.; Kamiya, K.; Pruksakorn, P.; Sumii, Y.; Kotoku, N.; Joubert, J.-P.; Moodley, P.; Han, C.; Shin, D.; Kobayashi, M.
    • Journal Title

      Bioorg. Med. Chem.

      Volume: 23 Issue: 13 Pages: 3534-3541

    • DOI

      10.1016/j.bmc.2015.04.033

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Aaptamines, marine spongean alkaloids, as anti-dormant mycobacterial substances.2014

    • Author(s)
      M. Arai, C. Han, Y. Yamano, A. Setiawan M. Kobayashi
    • Journal Title

      J. Nat. Med.

      Volume: 68 Issue: 2 Pages: 372-376

    • DOI

      10.1007/s11418-013-0811-y

    • NAID

      40020023317

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Identification of target protein of agelasine D, a marine spongean diterpene alkaloid, as an anti-dormant mycobacterial substance.2014

    • Author(s)
      M. Arai, Y. Yamano, A. Setiawan, M. Kobayashi
    • Journal Title

      ChemBioChem

      Volume: 15 Issue: 1 Pages: 117-123

    • DOI

      10.1002/cbic.201300470

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Marine derived fungal sesterterpenes, ophiobolins, inhibit biofilm formation of Mycobacterium species.2013

    • Author(s)
      M. Arai, H. Niikawa, M. Kobayashi
    • Journal Title

      J. Nat. Med.

      Volume: 67 Issue: 2 Pages: 271-275

    • DOI

      10.1007/s11418-012-0676-5

    • NAID

      40019608927

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potent growth inhibitory activity of (±)-platencin towards multi-drug-resistant and extensively drug-resistant Mycobacterium tuberculosis.2013

    • Author(s)
      G.A.I. Moustafa, S. Nojima, Y. Yamano, A. Aono, M. Arai, S. Mitarai, T. Tanaka, T. Yoshimitsu
    • Journal Title

      Med. Chem. Comm.

      Volume: 4 Issue: 4 Pages: 720-723

    • DOI

      10.1039/c3md00016h

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neamph°lide B, new cyclic depsipeptide, as an anti-dormant mycoba cterial substance from a Japanese marine sponge of Neamphius sp.2012

    • Author(s)
      Y. Yamano
    • Journal Title

      Bioorg. Med. Chem.Lett.

      Volume: 22(14) Pages: 4877-4881

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stylissamide X, a new proline-rich cyclic octapeptide as an inhibitor of cell migration, from an Indonesian marine sponge of Stylissa sp.2012

    • Author(s)
      M. Arai
    • Journal Title

      Bioorg. Med. Chem. Lett

      Volume: 22(4) Pages: 1818-1821

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Phenotypic screeningによる活性天然物探索と新規薬剤標的の開拓2015

    • Author(s)
      荒井雅吉
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      神戸学院大学(兵庫県・神戸市)
    • Year and Date
      2015-03-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 海洋由来放線菌が産生するnybomycinの潜在性結核菌に対する抗菌活性とその作用メカニズム2014

    • Author(s)
      ◯神谷謙太朗,住井裕司,申多英,Patamaporn Pruksakorn,韓智秀,古徳直之,小林資正
    • Organizer
      日本生薬学会第61年会
    • Place of Presentation
      福岡大学薬学部(福岡県・福岡市)
    • Year and Date
      2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Phenotypic Screening and Chemical Biology for Exploring New Medicinal Seeds and Drug Targets2014

    • Author(s)
      Masayoshi Arai
    • Organizer
      The 8th JSP-CCTCNM-KSP Joint Symposium on Pharmacognosy
    • Place of Presentation
      福岡大学薬学部(福岡県・福岡市)
    • Year and Date
      2014-09-13
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 病態部位の微小環境で作用する活性天然物の探索とその標的分子の解明2014

    • Author(s)
      荒井 雅吉
    • Organizer
      All Kitasato Project Study (AKPS) 共同研究最終報告会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 潜在性結核菌に有効な海綿由来テトラミン酸melophlin類の抗菌活性とその標的分子の解明2014

    • Author(s)
      神谷 謙太朗
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本
    • Related Report
      2013 Annual Research Report
  • [Presentation] 海綿由来ジテルペンアルカロイドagelasine類の潜在性結核菌に対する抗菌活性とその標的分子の解明2013

    • Author(s)
      荒井雅吉
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Year and Date
      2013-03-28
    • Related Report
      2012 Annual Research Report
  • [Presentation] 抗結核作用を有する天然有機化合物の研究2013

    • Author(s)
      荒井雅吉
    • Organizer
      岡山大学 難治性感染症を標的とした創薬研究教育推進事業
    • Place of Presentation
      岡山大学薬学部(岡山)
    • Year and Date
      2013-02-08
    • Related Report
      2012 Annual Research Report
  • [Presentation] Phenotypic screeningによる医薬シーズ探索と標的分子の解明2013

    • Author(s)
      荒井 雅吉
    • Organizer
      第48回天然物談話会
    • Place of Presentation
      滋賀
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Search for anti-dormant mycobacterial substances from marine organisms and analysis of their target molecules.2013

    • Author(s)
      Masayoshi Arai
    • Organizer
      JSPS Vietnam-Japan Joint Symposium
    • Place of Presentation
      Osaka
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 潜在性結核菌に有効なジテルペンアルカロイドagelasine Dの結合タンパク質の解明2013

    • Author(s)
      小林 資正
    • Organizer
      日本生薬学会第60回年会
    • Place of Presentation
      北海道
    • Related Report
      2013 Annual Research Report
  • [Presentation] 病態の体内微小環境で機能する活性天然物の探索とその標的分子の解明2013

    • Author(s)
      荒井 雅吉
    • Organizer
      日本生薬学会第60回年会
    • Place of Presentation
      北海道
    • Related Report
      2013 Annual Research Report
  • [Presentation] 海綿由来テトラミン酸melophlin類の潜在性結核菌に対する抗菌活性2013

    • Author(s)
      荒井 雅吉
    • Organizer
      第63回日本薬学会近畿支部大会
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] 海洋生物由来の抗潜在性結核物質の探索とその作用メカニズム解析2012

    • Author(s)
      山野喜
    • Organizer
      第19回天然薬物の開発と応用シンポジウム
    • Place of Presentation
      大阪大学会館(大阪)
    • Year and Date
      2012-11-02
    • Related Report
      2012 Annual Research Report
  • [Presentation] 潜在性結核菌に有効な抗菌物質の探索とゲノムDNAライブラリーを利用する標的分子の解明2012

    • Author(s)
      荒井雅吉
    • Organizer
      第54回天然有機化合物討論会
    • Place of Presentation
      東京農業大学 世田谷キャンパス(東京)
    • Year and Date
      2012-09-19
    • Related Report
      2012 Annual Research Report
  • [Presentation] 海綿由来ジテルペンアルカロイドagelasine類の抗潜在性結核活性とその標的分子の解析2012

    • Author(s)
      荒井雅吉
    • Organizer
      日本生薬学会 第59回年会
    • Place of Presentation
      かずさアカデミアパーク(千葉)
    • Year and Date
      2012-09-17
    • Related Report
      2012 Annual Research Report
  • [Presentation] 海洋由来真菌が産生するセスタテルペンophiobolin類のbiof ilm形成阻害活性2012

    • Author(s)
      小林資正
    • Organizer
      日本生薬学会 第59回年会
    • Place of Presentation
      かずさアカデミアパーク(千葉)
    • Year and Date
      2012-09-17
    • Related Report
      2012 Annual Research Report
  • [Presentation] Search for anti-dormant mycobacterial substances from marine sponges and analysis of their action-mechanism.2012

    • Author(s)
      Yoshi Yamano
    • Organizer
      The 7th Seoul-Kyoto-Osaka Joint Symposium on Pharmaceutical Sciences for Young Scientists
    • Place of Presentation
      京都大学薬学部(京都)
    • Year and Date
      2012-05-25
    • Related Report
      2012 Annual Research Report
  • [Book] Search for New Medicinal Seeds from Marine Organisms. In; Chembiomol ecular Science : at the Frontier of Chemist and Biology2013

    • Author(s)
      M, Kobayashi
    • Total Pages
      325
    • Publisher
      Springer-Verlag GmbH
    • Related Report
      2012 Annual Research Report
  • [Remarks] 大阪大学大学院 薬学研究科 天然物化学分野 ホームページ

    • URL

      http://www.phs.osaka-u.ac.jp/homepage/b012/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 大阪大学大学院 薬学研究科 天然物化学分野

    • URL

      http://www.phs.osaka-u.ac.jp/homepage/b012/

    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://www.phs.osaka-u.ac.jp/homepage/b012/

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi