Search for bioactive natural products acting at infected region of M. tuberculosis and analysis of their target molecules
| Project/Area Number |
24310159
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Osaka University |
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Principal Investigator |
Arai Masayoshi 大阪大学, 薬学研究科(研究院), 准教授 (80311231)
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| Co-Investigator(Renkei-kenkyūsha) |
KOBAYASHI Motomasa 大阪大学, 大学院薬学研究科, 教授 (40116033)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | 抗菌物質 / 結核 / 感染症 / 活性天然物 / ケミカルバイオロジー / 抗生物質 |
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| Outline of Final Research Achievements |
In order to search for new anti-microbial substances against the dormant Mycobacterium tuberculosis, which shows tolerance to the conventional anti-Tuberculosis drugs, we constructed several screening systems focusing on the characteristic phenotypes of mycobacteria at the infected region. On the guidance of the screening from the extract library of marine sponges and marine-derived microbes, we could explore several anti-microbial compounds including structurally new compounds, which are expected to be a new medicinal seed. In addition, we could clarify the target molecules of some isolated anti-microbial compounds by the method utilizing the transformants overexpressing genomic DNA library, the analysis of gene mutation and/or the pull-down assay using a synthetic probe of the active compound. The clarified target proteins are expected to become a new drug target against the dormant M. tuberculosis.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Anti-dormant mycobacterial activity and target analysis of nybomycin produced by a marine-derived Streptomyces sp.2015
Author(s)
Arai, M.; Kamiya, K.; Pruksakorn, P.; Sumii, Y.; Kotoku, N.; Joubert, J.-P.; Moodley, P.; Han, C.; Shin, D.; Kobayashi, M.
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Journal Title
Bioorg. Med. Chem.
Volume: 23
Pages: 3534-3541
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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