|Budget Amount *help
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
|Outline of Final Research Achievements
We previously elucidated the mechanism of aberrant splicing of the presenilin-2 pre-mRNA, specifically observed in the patients of sporadic Alzheimer’s disease. In this study, we tried to solve the functional structure, HMGA1α-U1 snRNP-target RNA complex. The interactions were analyzed by a highly sensitive isothermal titration calorimeter and ES-mass spectrometer. Intriguingly, we found that HMGA1α is also involved in the specific alternative splicing of Estrogen receptor α (ERα) pre-mRNA and AIDS virus (HIV-1) transcript. The shared mechanism was that the HMGA1α binds to the target sequence and interferes with the adjacent 5′ splice site via aberrant binding of U1 snRNP, the 5′ splice site recognition factor.