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Structural probing of RNA molecules with in-cell NMR

Research Project

Project/Area Number 24310163
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Chemical biology
Research InstitutionTokushima Bunri University (2015)
Tohoku University (2012-2014)

Principal Investigator

TANAKA Yoshiyuki  徳島文理大学, 薬学部, 教授 (70333797)

Co-Investigator(Kenkyū-buntansha) KOJIMA Chojiro  横浜国立大学, 大学院工学研究科, 教授 (50333563)
Co-Investigator(Renkei-kenkyūsha) TOCHIO Hidehito  京都大学, 大学院理学研究科, 教授 (70336593)
ONO Akira  神奈川大学, 工学部, 教授 (10183253)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords生体分子計測 / NMR / 安定同位体標識 / バイオプローブ / 細胞内化学反応
Outline of Final Research Achievements

In-cell NMR has been rarely applied to nucleic acids. In order to apply in-cell NMR into nucleic acids, there are several issues. 1) There has been no appropriate site-specific labeling technique of long RNA molecules. 2) There was no established method for introducing nucleic acids into cells. Therefore, at first, Tanaka (principal investigator: PI) developed a site-specific labeling technique of RNA molecules by using a recently discovered primer-extension ability of RNA polymerases. Interestingly, by using the derived labeled RNA molecule (HAC1 mRNA), PI identified that conserved C883 and G888 residues were base-paired within a loop. Then, in collaboration with grant team members, a method for introducing RNA molecules into cells was established by using an electroporation technique. It should be also mentioned that chemical reactions occurred to the introduced molecule was detected by using appropriate 19F-labeling tag. Thus, the team accomplished main purposes of this project.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (48 results)

All 2016 2015 2014 2013 2012 Other

All Int'l Joint Research (2 results) Journal Article (23 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 20 results,  Open Access: 2 results,  Acknowledgement Compliant: 3 results) Presentation (23 results) (of which Int'l Joint Research: 2 results,  Invited: 4 results)

  • [Int'l Joint Research] チェコ科学アカデミー(チェコ)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] VU University Amsterdam(ドイツ)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Benchmark Theoretical and Experimental Study on 15N NMR Shifts of Oxidatively Damaged Guanine2016

    • Author(s)
      Martin Dracinsky, Michal Sala, Blanka Klepetarova, Jakub Sebera, Jiri Fukal, Veronika Holeckova, Yoshiyuki Tanaka, Radim Nencka, and Vladimir Sychrovsky
    • Journal Title

      Journal of Physical Chemistry B

      Volume: 120 Issue: 5 Pages: 915-925

    • DOI

      10.1021/acs.jpcb.5b11428

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Direct Detection of the Mercury-Nitrogen Bond in the Thymine-HgII-Thymine Base-pair with 199Hg NMR Spectroscopy2015

    • Author(s)
      Takenori Dairaku, Kyoko Furuita, Hajime Sato, Jakub Sebera, Daichi Yamanaka, Hiroyuki Otaki, Shoko Kikkawa, Yoshinori Kondo, Ritsuko Katahira, F. Matthias Bickelhaupt, Celia Fonseca Guerra, Akira Ono, Vladimir Sychrovsky, Chojiro Kojima and Yoshiyuki Tanaka
    • Journal Title

      Chemical Communications

      Volume: 51 Issue: 40 Pages: 8488-8491

    • DOI

      10.1039/c5cc02423d

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Exploring a DNA sequence for the three-dimensional structure determination of a silver(I)-mediated C-C base pair in a DNA duplex by 1H NMR spectroscopy2015

    • Author(s)
      Takenori Dairaku, Kyoko Furuita, Hajime Sato, Yoshinori Kondo, Chojiro Kojima, Akira Ono and Yoshiyuki Tanaka
    • Journal Title

      Nucleosides, Nucleotides & Nucleic Acids

      Volume: 34 Issue: 12 Pages: 877-900

    • DOI

      10.1080/15257770.2015.1088160

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] High-resolution crystal structure of AgI-RNA hybrid duplex containing Watson-Crick-like C-AgI-C metallo-base pairs2015

    • Author(s)
      Jiro Kondo, Yoshinari Tada, Takenori Dairaku, Hisao Saneyoshi, Itaru Okamoto, Yoshiyuki Tanaka, Akira Ono
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 54 Issue: 45 Pages: 13323-13326

    • DOI

      10.1002/anie.201507894

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structures, physicochemical properties, and applications of T-HgII-T, C-AgI-C, and other metallo-base-pairs.2015

    • Author(s)
      Yoshiyuki Tanaka, Jiro Kondo, Vladimir Sychrovsky, Jakub Sebera, Takenori Dairaku, Hisao Saneyoshi, Hidehito Urata, Hidetaka Torigoe and Akira Ono
    • Journal Title

      Chem. Commun.

      Volume: 51 Issue: 98 Pages: 17313-17360

    • DOI

      10.1039/c5cc02693h

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The activation of N-glycosidic bond cleavage operated by base-excision repair enzyme hOGG1; theoretical study of the role of Lys 249 residue in activation of G, OxoG and FapyG.2014

    • Author(s)
      Jakub Sebera, Lukas Trantirek, Yoshiyuki Tanaka, Radim Nencka, Jiri Fukal, Vladimir Sychrovsky
    • Journal Title

      RSC Advances

      Volume: 4 Issue: 83 Pages: 44043-44051

    • DOI

      10.1039/c4ra08278h

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pseudoknot interaction-mediated activation of hammerhead ribozyme which is applicable to gene-therapeutic agents2014

    • Author(s)
      Mituhiro Kuriyama, Yoshinori Kondo, and Yoshiyuki Tanaka
    • Journal Title

      Nucleosides Nucleotides Nucleic Acids

      Volume: (印刷中)(掲載確定) Issue: 7 Pages: 466-480

    • DOI

      10.1080/15257770.2014.887098

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hg<sup>2+</sup>-Trapping Beads: Hg<sup>2+</sup>-Specific Recognition through Thymine–Hg(II)–Thymine Base Pairing2014

    • Author(s)
      栗山光博、他12名
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: 62 Issue: 7 Pages: 709-712

    • DOI

      10.1248/cpb.c13-00918

    • NAID

      130004053812

    • ISSN
      0009-2363, 1347-5223
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Mechanism of base excision with the hOGG1 DNA repair enzyme.2014

    • Author(s)
      Jakub Sebera, Lukas Trantirek, Jiri Fukal, Yoshiyuki Tanaka and Vladimir Sychrovsky
    • Journal Title

      Chemicke Listy

      Volume: 108 Pages: 364-367

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Theoretical and experimental study of charge transfer through DNA: impact of mercury mediated T-Hg-T base pair.2014

    • Author(s)
      Irena Kratochvilova, Martin Golan, Martin Vala, Miroslava Sperova, Martin Weiter, Ondrej Pav, Jakub Sebera, Ivan Rosenberg, Vladimir Sychrovsky, Yoshiyuki Tanaka and F. Matthias Bickelhaupt
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 118 Issue: 20 Pages: 5374-5381

    • DOI

      10.1021/jp501986a

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NMR spectroscopic studies on metallo-base-pair in DNA duplex.2014

    • Author(s)
      Yoshiyuki Tanaka, Itaru Okamoto, Kyoko Furuita, Jakub Sebera, Jiro Kondo, Hidetaka Torigoe, Hidehito Urata, Takenori Dairaku, Akira Ono, Chojiro Kojima, Vladimir Sychrovsky
    • Journal Title

      Journal of Biological Inorganic Chemistry

      Volume: 19 Issue: S2 Pages: 725-747

    • DOI

      10.1007/s00775-014-1157-y

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Syntheses of DNA duplexes containing metal ion mediated base pairs.2014

    • Author(s)
      Akira Ono, Itaru Okamoto, Hidehito Urata, Hidetaka Torigoe, Hisao Saneyoshi, Jiro Kondo, Yoshiyuki Tanaka
    • Journal Title

      Journal of Biological Inorganic Chemistry

      Volume: 19 Issue: S2 Pages: 713-724

    • DOI

      10.1007/s00775-014-1156-z

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Proton and nitrogen-15 NMR spectroscopic studies of AgI-mediated C-C base-pairs.2014

    • Author(s)
      Takenori Dairaku, Itaru Okamoto, Kyoko Furuita, Shuji Oda, Daichi Yamanaka, Hidetaka Torigoe, Tetsuo Kozasa, Yoshinori Kondo, Akira Ono, Chojiro Kojima, Vladimir Sychrovsky, Yoshiyuki Tanaka
    • Journal Title

      Journal of Biological Inorganic Chemistry

      Volume: 19 Issue: S2 Pages: 815-832

    • DOI

      10.1007/s00775-014-1163-0

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Crystal structure of metallo-DNA duplex containing consecutive Watson-Crick-like T-Hg(II)-T base pairs2014

    • Author(s)
      Jiro Kondo, Tom Yamada, Chika Hirose, Itaru Okamoto, Yoshiyuki Tanaka, Akira Ono
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 53 Issue: 9 Pages: 2385-2388

    • DOI

      10.1002/anie.201309066

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The structure of metallo-DNA with consecutive T-HgII-T base-pairs explains positive entropy for the metallo-base-pair formation2014

    • Author(s)
      Yamaguchi H
    • Journal Title

      Nucleic Acids Res.

      Volume: 42 Issue: 6 Pages: 4094-4099

    • DOI

      10.1093/nar/gkt1344

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Formation of the T-HgII-T Metal-Mediated DNA Base Pair; Proposal and Theoretical Calculation of the Reaction Pathway2013

    • Author(s)
      Sebera J
    • Journal Title

      Chemistry

      Volume: 19 Issue: 30 Pages: 9884-9894

    • DOI

      10.1002/chem.201300460

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Organocatalytic functionalization of heteroaromatic N-oxides with C-nucleophiles using in situ generated onium amide bases2013

    • Author(s)
      Kiyofumi Inamoto, Yuta Araki, Shoko Kikkawa, Misato Yonemoto, Yoshiyuki Tanaka, Yoshinori Kondo
    • Journal Title

      Organic &Biomolecular Chemistry

      Volume: 11 Issue: 27 Pages: 4438-4441

    • DOI

      10.1039/c3ob40782a

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Carboxylation of alkynylsilanes with carbon dioxide mediated by cesium fluoride in DMSO2013

    • Author(s)
      Misato Yonemoto-Kobayashi, Kiyofumi Inamoto, Yoshiyuki Tanaka and Yoshinori Kondo
    • Journal Title

      Organic & Biomolecula Chemistry

      Volume: 11 Issue: 23 Pages: 3773-3775

    • DOI

      10.1039/c3ob40760h

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of Mercury-TpT Dinucleotide Binding by the Raman Spectra. A Computational Study2013

    • Author(s)
      Ladislav Benda, 他4名(最終著者)
    • Journal Title

      The Journal of Physical Chemisty A

      Volume: 116 Issue: 32 Pages: 8313-8320

    • DOI

      10.1021/jp3045077

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pyramidalization of the Glycosidic Nitrogen Provides the Way for Efficient Cleavage of the N-glycosidic Bond of 8-OxoG with the hOGGl DNA Repair Protein2013

    • Author(s)
      Jakub Sebera, Jakub Sebera, Lukas Tranti'rek, 田中 好幸, Vladimir Sychrovsky
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 116 Issue: 41 Pages: 12535-12544

    • DOI

      10.1021/jp309098d

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure Formation and Catalytic Activity of DNA in Organic Solvents2012

    • Author(s)
      Hiroshi Abe, Naoko Abe, Aya Shibata, Keiji Ito, Yoshiyuki Tanaka, Mika Ito, Hisao Saneyoshi, Satoshi Shuto, and Yoshihiro Ito
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 51 Issue: 26 Pages: 6475-6479

    • DOI

      10.1002/anie.201201111

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Raman spectroscopic detection of the T-HgII-T base pair and the ionic characteristics of mercury2012

    • Author(s)
      Tomomi Uchiyama, Takashi Miura, Hideo Takeuchi, Takenori Dairaku, Tomoyuki Komuro, Takuya Kawamura, Yoshinori Kondo, Ladislav Benda, Vladimir Sychrovsky, Petr Bour, Itaru Okamoto, Akira Ono and Yoshiyuki Tanaka
    • Journal Title

      Nucleic Acids Res

      Volume: 40 Issue: 12 Pages: 5766-5774

    • DOI

      10.1093/nar/gks208

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thermodynamic and structural properties of the specific binding between Ag+ ion and C:C mismatched base pair in duplex DNA to form C-Ag-C metal-mediated base pair2012

    • Author(s)
      Hidetaka Torigoe
    • Journal Title

      Biochimie

      Volume: 94 Issue: 11 Pages: 2431-2440

    • DOI

      10.1016/j.biochi.2012.06.024

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] C-Ag(I)-C塩基対を含むメタロDNAの立体構造解析2015

    • Author(s)
      古板 恭子, 大樂 武範, 児嶋 長次郎, 田中 好幸
    • Organizer
      第54回NMR討論会
    • Place of Presentation
      習志野市
    • Year and Date
      2015-11-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] Structure of C-Ag(I)-C metallo base pair in RNA duplex2015

    • Author(s)
      Yoshinari Tada, Takenori Dairaku, Itaru Okamoto, Hisao Saneyoshi, Yoshiyuki Tanaka, Akira Ono, Jiro Kondo
    • Organizer
      第42回国際核酸化学シンポジウム
    • Place of Presentation
      姫路
    • Year and Date
      2015-09-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] NMR structure of a DNA duplex containing a silver-mediated C-C base pair2015

    • Author(s)
      Kyoko Furuita, Takenori Dairaku, Chojiro Kojima, Yoshiyuki Tanaka
    • Organizer
      第42回国際核酸化学シンポジウム
    • Place of Presentation
      姫路
    • Year and Date
      2015-09-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] NMR spectroscopic studies on metallo-base-pair in DNA duplex.2014

    • Author(s)
      ◯Yoshiyuki Tanaka, Itaru Okamoto, Kyoko Furuita, Jakub Sebera, Jiro Kondo, Hidetaka Torigoe, Hidehito Urata, Takenori Dairaku, Akira Ono, Chojiro Kojima, Vladimir Sychrovsky
    • Organizer
      12th European Biological Inorganic Chemistry Conference (EuroBIC-12)
    • Place of Presentation
      チューリッヒ、スイス
    • Year and Date
      2014-08-24 – 2014-08-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Syntheses of DNA duplexes containing metal ion mediated base pairs2014

    • Author(s)
      ◯Akira Ono, Itaru Okamoto, Hidehito Urata, Hidetaka Torigoe, Hisao Saneyoshi, Jiro Kondo, Yoshiyuki Tanaka
    • Organizer
      12th European Biological Inorganic Chemistry Conference (EuroBIC-12)
    • Place of Presentation
      チューリッヒ、スイス
    • Year and Date
      2014-08-24 – 2014-08-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Proton and nitrogen-15 NMR spectroscopic studies of AgI-mediated C-C base-pairs.2014

    • Author(s)
      ◯Takenori Dairaku, Itaru Okamoto, Kyoko Furuita, Shuji Oda, Daichi Yamanaka, Hidetaka Torigoe, Tetsuo Kozasa, Yoshinori Kondo, Akira Ono, Chojiro Kojima, Vladimir Sychrovsky, Yoshiyuki Tanaka
    • Organizer
      12th European Biological Inorganic Chemistry Conference (EuroBIC-12)
    • Place of Presentation
      チューリッヒ、スイス
    • Year and Date
      2014-08-24 – 2014-08-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 多核NMR分光法による機能性核酸分子の局所観測およびJ値・化学シフト値による状態解析2014

    • Author(s)
      田中好幸
    • Organizer
      第15回若手NMR研究会
    • Place of Presentation
      千葉県長生郡長柄町
    • Year and Date
      2014-07-12
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 化学シフト値・結合定数J(金属錯体の配位化学・構造化学)2013

    • Author(s)
      田中好幸
    • Organizer
      日本分光学会NMR部会2013年NMR講習会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 水銀イオンのT-Tミスマッチ塩基対に対する親和性の構造化学的要因(口頭発表)2013

    • Author(s)
      ◯田中好幸, 大樂武範, 根東義則, 内山朋美, 三浦隆史, 竹内英夫, 古板恭子, 児嶋長次郎, Ladislav Benda, Jakub Sebera, Vladimir Sychrovsky, 岡本到, 小野晶
    • Organizer
      第回 生体分子科学討論会
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
  • [Presentation] DNAを用いた水銀除去剤の研究(口頭発表)2013

    • Author(s)
      ◯栗山 光博、春田 佳一郎、小野 晶、森田 勇人、根東 義則、田中 好幸
    • Organizer
      第7回 バイオ関連シンポジウム
    • Place of Presentation
      名古屋
    • Related Report
      2013 Annual Research Report
  • [Presentation] 金属内包DNAナノワイヤー創成に向けたメタロ塩基対の構造解析(口頭発表)2013

    • Author(s)
      ◯大樂武範、岡本到、古板恭子、Vladimir Sychrovsky、山中大地、織田修司、児嶋長次郎、小野晶、根東義則、田中好幸
    • Organizer
      第51回 日本薬学会東北支部大会
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] 部位特異的RNA切断酵素Ire1pによるHAC1 mRNA 切断機構の解析2013

    • Author(s)
      ◯河原郁美、芦原悠太、春田佳一郎、児嶋長次郎、田中好幸
    • Organizer
      第51回 日本生物物理学会年会
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] Structure of Ag(I)-mediated C-C base pair determined by hetero-nuclear NMR spectroscopy2013

    • Author(s)
      Takenori Dairaku, Itaru Okamoto, Kyoko Furuita, Jukub Sebera, Mathias Bickelhaupt, Shuji Oda, Daichi Yamanaka, Yoshinori Kondo, Vladimir Sychrovsky, Chojiro Kojima, Akira Ono, ◯Yoshiyuki Tanaka
    • Organizer
      40th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] Construction of expression system of human 8-oxoguanine glycosylase 1 for its mechanistic analysis2013

    • Author(s)
      ◯Daichi Sato, Momoko Yoneyama, Ikumi Kawahara, Kyoko Fruita, Yoshinori Kondo, Chojiro Kojima, Yoshiyuki Tanaka
    • Organizer
      40th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] Hammerhead ribozyme which is responsible for a point mutation in a substrate RNA2013

    • Author(s)
      ◯Mituhiro Kuriyama, Hisaaki Tateoka, Yoshinori Kondo, Yoshio Kato, and Yoshiyuki Tanaka
    • Organizer
      40th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] NMR analysis of HAC1 mRNA using site-specific isotope labeling2013

    • Author(s)
      ◯Ikumi Kawahara, Yuta Ashihara, Kaichiro Haruta, Yoshiyuki Tanaka and Chojiro Kojima
    • Organizer
      40th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] Site-specific labeling techniques of RNA molecules for structural and mechanistic studies with NMR spectroscopy2012

    • Author(s)
      田中好幸, 他6名
    • Organizer
      39th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      名古屋, 日本(国際会議)(口頭発表)
    • Year and Date
      2012-11-17
    • Related Report
      2012 Annual Research Report
  • [Presentation] 高分子量機能性RNAの局所構造・状態解析のため部位特異的標識法2012

    • Author(s)
      田中好幸, 他4名
    • Organizer
      第51回NMR討論会
    • Place of Presentation
      名古屋(口頭発表)
    • Year and Date
      2012-11-10
    • Related Report
      2012 Annual Research Report
  • [Presentation] NMR分光法を利用したHammerhead Ribozymeの活性残基の物性解析2012

    • Author(s)
      山中大地, 他3名
    • Organizer
      第51回日本薬学会東北支部大会
    • Place of Presentation
      青森(口頭発表)
    • Year and Date
      2012-10-07
    • Related Report
      2012 Annual Research Report
  • [Presentation] シュードノット構造を持つハンマーヘッド型リボザイムを利用した遺伝子治療薬創製研究2012

    • Author(s)
      栗山光博, 他4名
    • Organizer
      アンチセンス・遺伝子・デリバリーシンポジウム
    • Place of Presentation
      仙台(ポスター)
    • Year and Date
      2012-09-17
    • Related Report
      2012 Annual Research Report
  • [Presentation] NMR分光法によるRNA分子の局所構造プローブ法の開発2012

    • Author(s)
      芦原悠太, 他7名
    • Organizer
      14th RNA meeting in Tohoku
    • Place of Presentation
      仙台(ポスター)
    • Year and Date
      2012-07-19
    • Related Report
      2012 Annual Research Report
  • [Presentation] スプライソソーム非依存的スプライシングを可能にするHACL mRNAの構造特性2012

    • Author(s)
      河原郁美, 他4名
    • Organizer
      14th RNA meeting in Tohoku
    • Place of Presentation
      仙台(ポスター)
    • Year and Date
      2012-07-18
    • Related Report
      2012 Annual Research Report
  • [Presentation] 小胞体ストレスセンサーIrelpにより細胞質スプライシングを受けるHACL mRNAの構造解析2012

    • Author(s)
      田中好幸, 他4名
    • Organizer
      第39回生体分子科学討論会
    • Place of Presentation
      仙台(口頭発表)
    • Year and Date
      2012-06-09
    • Related Report
      2012 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

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