Biological molecular layers in aqueous environments studied by high-sensitive vibrational SFG spectroscopy
Project/Area Number |
24350010
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Physical chemistry
|
Research Institution | University of Tsukuba |
Principal Investigator |
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
|
Keywords | 非線形分光 / 振動スペクトル / 界面 / 生体分子 / 単分子膜 |
Outline of Final Research Achievements |
Several techniques for high-sensitive vibrational sum-frequency generation (SFG) spectroscopy to investigate biological molecular layers in aqueous conditions have been developed or examined. The techniques include (1) high-sensitive heterodyne-detected vibrational SFG spectrometer with a very-thin quartz crystal as the local oscillator, (2) nano-particle enhanced vibrational SFG, and (3) prism-parallel substrate coupled systems for vibrational SFG at total reflection geometries. As applications, surface-pressure dependent structures of lipid layers on water and silica substrates (Langmuir and Langmuir-Blodgett monolayers) and molecular orientations and structures of proteins and water at surface of proteins aqueous solutions have been investigated.
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Report
(4 results)
Research Products
(19 results)