Development of high performance bioanalytical techniques by capillary/microchip separation and detection systems

• Project/Area Number: 24350039
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Kyoto University
• Principal Investigator: OTSUKA Koji 京都大学, 工学(系)研究科(研究院), 教授 (70183762)
• Co-Investigator(Kenkyū-buntansha): KUBO Takuya 京都大学, 工学研究科, 准教授 (20374994) ; NAITO Toyohiro 京都大学, 工学研究科, 助教 (10711806) ; 末吉 健志 京都大学, 工学研究科, 助教 (70552660)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2012: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
• Keywords: 分離科学 / キャピラリー / マイクロチップ / 電気泳動 / クロマトグラフィー / バイオ分析 / 分離検出 / 糖鎖

Outline of Final Research Achievements

Development of high performance bio-separation and analysis systems based on capillary electrophoresis (CE) and microchip electrophoresis (MCE) was investigated, where developments of high performance separation and detection systems using online sample concentration techniques and mass spectrometry (MS) and novel separation fields for separations of proteins and carbohydrates were mainly carried out. Following four categories were most important issues: 1) progress of high performance online sample concentration techniques; 2) improvement of the performance of MCE-MS using an interface integrated microchip with a separation channel for MCE; 3) development of CE/MCE-MS using online sample concentration techniques; 4) improving the performance of CE/MCE with novel selective separation fields.

Research Products

• [Journal Article] Hydrodynamic Nonadhesive Cell Retaining in a Microfluidic Circuit for Stress-less Suspension Culture (2015)
  • Author(s): Naito, T.; Kaji, N.; Tokeshi, M.; Kubo, T.; Baba, Y.; Otsuka, K.
  • Journal Title: Anal. Methods Volume: -
  • Peer Reviewed
• [Journal Article] Simple and Effective Label-Free Capillary Electrophoretic Analysis of Sugars by Complexation Using Quinoline Boronic Acids (2015)
  • Author(s): Kubo, T.; Kanemori, K.; Kusumoto, R.; Kawai, T.; Sueyoshi, K.; Naito, T.; Otsuka, K.
  • Journal Title: Anal. Chem. Volume: 87
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hydrophilic Interaction Electrokinetic Chromatography Using Bio-based Nanofillers (2014)
  • Author(s): Takayuki Kawai, Masato Watanabe, Kojiro Uetani, Yudai Fukushima, Kenji Sueyoshi, Takuya Kubo, Fumihiko Kitagawa, Hiroyuki Yano, Koji Otsuka
  • Journal Title: Electrophoresis Volume: 35 Issue: 15 Pages: 2229-2236
  • DOI: 10.1002/elps.201300558
  • Peer Reviewed / Open Access
• [Journal Article] Quantitative Ligand Immobilization Using Alginate Hydrogel Formed in a Capillary: Application for On-line Affinity Concentration (2014)
  • Author(s): Fukushima, Y.; Naito, T.; Sueyoshi, K.; Kubo, T.; Kitagawa, F.; Otsuka, K.
  • Journal Title: Anal. Chem. Volume: 86 Issue: 12 Pages: 5977-5982
  • DOI: 10.1021/ac501039j
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Tunable Molecular Sieving in Gel Electrophoresis Using a Poly(ethylene glycol)-Based Hydrogel (2014)
  • Author(s): Kubo, T.; Oketani, M.; Tominaga, Y.; Naito, T.; Otsuka, K.
  • Journal Title: CHROMATOGRAPHY Volume: 35 Issue: 2 Pages: 81-86
  • DOI: 10.15583/jpchrom.2014.009
  • NAID: 130005057716
  • ISSN: 1342-8284, 1348-3315
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Molecularly Imprinted Adsorbents for Selective Separation and/or Concentration of Environmental Pollutants (2014)
  • Author(s): Kubo, T.; Hosoya, K.; Otsuka, K.
  • Journal Title: Analytical Sciences Volume: 30 Issue: 1 Pages: 97-104
  • DOI: 10.2116/analsci.30.97
  • NAID: 130004442252
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] アルカリ金属イオンおよびアンモニウムイオンの蛍光センシングのための量子ドットの作製 (2014)
  • Author(s): 北川文彦, 甲斐美奈子, 大塚浩二, 分析化学 2014, 63,943-949
  • Journal Title: 分析化学 Volume: 63 Pages: 943-949
  • Peer Reviewed / Open Access
• [Journal Article] Sensitivity Enhancement by Sweeping via Borate Complexation in Capillary Electrophoretic Analysis of Glycoproteins (2014)
  • Author(s): Kitagawa, F.; Nojiri, M.; Sueyoshi, K.; Otsuka, K.
  • Journal Title: CHROMATOGRAPHY Volume: 35 Issue: 3 Pages: 125-129
  • DOI: 10.15583/jpchrom.2014.020
  • NAID: 130004715565
  • ISSN: 1342-8284, 1348-3315
  • Peer Reviewed / Open Access
• [Journal Article] Recent applications of on-line sample preconcentration techniques in capillary electrophoresis (2014)
  • Author(s): Kitagawa, F.; Otsuka, K.
  • Journal Title: J. Chromatogr. A Volume: 1335 Pages: 43-60
  • DOI: 10.1016/j.chroma.2013.10.066
  • Peer Reviewed
• [Journal Article] Effective determination of a pharmaceutical, sulpiride, in river water by online SPE-LC-MS using a molecularly imprinted polymer as a preconcentration medium (2014)
  • Author(s): Kubo, T.; Kuroda, K.; Tominaga, Y.; Naito, T.; Sueyoshi, K.; Hosoya, K.; Otsuka, K.
  • Journal Title: J. Pharm. Biomed. Anal. Volume: 89 Pages: 111-117
  • DOI: 10.1016/j.jpba.2013.10.040
  • Peer Reviewed
• [Journal Article] Development of a C60-fullerene bonded open-tubular capillary using a photo/thermal active agent for liquid chromatographic separations by p-p interactions (2014)
  • Author(s): Kubo, T.; Murakami, Y.; Tominaga, Y.; Naito, T.; Sueyoshi, K.; Yan, M.; Otsuka, K.
  • Journal Title: J. Chromatogr. A Volume: 1323 Pages: 174-178
  • DOI: 10.1016/j.chroma.2013.10.097
  • Peer Reviewed
• [Journal Article] Solvent induced nanostructure formation in polymer thin films: the impact of oxidation and solvent (2014)
  • Author(s): Kubo, T.; Im, J.; Wang, X.; Whitten, J. E.; Otsuka, K.; Yan, M.
  • Journal Title: Colloids Surf. A Volume: 444 Pages: 217-225
  • DOI: 10.1016/j.colsurfa.2013.12.052
  • Peer Reviewed
• [Journal Article] Trace level determination of polycyclicaromatic hydrocarbons in river water withautomated pretreatment HPLC (2013)
  • Author(s): Y. Watabe, T. Kubo, T. Tanigawa, Y. Hayakawa, K. Otsuka, andK. Hosoya
  • Journal Title: J. Sep. Sci. Volume: 36 Issue: 6 Pages: 1128-1134
  • DOI: 10.1002/jssc.201201096
  • Peer Reviewed
• [Journal Article] Antibacterial Activities Effectuated by Co-continuous Epoxy-based Polymer Materials (2013)
  • Author(s): T. Kubo, K. Yasuda, Y. Tominaga, K. Otsuka, K. Hosoya
  • Journal Title: Colloids and Surfaces B Volume: 107 Pages: 53-58
  • DOI: 10.1016/j.colsurfb.2013.01.043
  • Peer Reviewed
• [Journal Article] Hybridization of a Macroporous Sponge and Spherical Microporous Adsorbents for High Throughput Separation of Ionic Solutes (2013)
  • Author(s): T. Kubo, K. Yasuda, Tominaga, K. Hosoya、K. Otsuka
  • Journal Title: Analytical Sciences Volume: 29 Issue: 4 Pages: 417-421
  • DOI: 10.2116/analsci.29.417
  • NAID: 10031165332
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed
• [Journal Article] Synthesis of Poly(ethylene glycol)-Based Hydrogels and Their Swelling/Shrinking Response to Molecular Recognition (2013)
  • Author(s): Tominaga, Y.; Kubo, T.; Sueyoshi, K.; Hosoya, K.; Otsuka, K.
  • Journal Title: J. Polym. Sci. Part A: Polym. Chem. Volume: 51 Issue: 15 Pages: 3153-3158
  • DOI: 10.1002/pola.26700
  • Peer Reviewed
• [Journal Article] Rapid Separations on Liquid Chromatography Using Ion Exchangeable Media Based on a Spongy Monolith (2013)
  • Author(s): Kubo, T.; Tanigawa, T.; Tominaga, Y.; Hosoya, K.; Otsuka, K.
  • Journal Title: J. Sep. Sci. Volume: 36 Issue: 17 Pages: 2813-2818
  • DOI: 10.1002/jssc.201300392
  • Peer Reviewed
• [Journal Article] On-line Sample Preconcentration by Large-volume Sample Stacking with an Electroosmotic Flow Pump (LVSEP) in Microscale Electrophoresis (2013)
  • Author(s): 北川 文彦, 川井 隆之, 大塚 浩二
  • Journal Title: Analytical Sciences Volume: 29 Issue: 12 Pages: 1129-1139
  • DOI: 10.2116/analsci.29.1129
  • NAID: 130004827317
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed
• [Journal Article] Inner surface modification of poly(dimethylsiloxane) microchannel for electrophoretic analysis of proteins (2013)
  • Author(s): 末吉健志, 堀祐輔, 大塚浩二
  • Journal Title: Microfluid. Nanofluid. Volume: (印刷中) Issue: 6 Pages: 933-941
  • DOI: 10.1007/s10404-012-1100-x
  • Peer Reviewed
• [Journal Article] Zone electrophoresis of proteins in poly(dimethylsiloxane) (PDMS) microchip coated with physically adsorbed amphiphilic phospholipid polymer (2013)
  • Author(s): Kyosuke Nii, Kenji Sueyoshi , Koji Otsuka, Madoka Takai
  • Journal Title: Microfluid Nanofluid Volume: 14 Issue: 6 Pages: 951-959
  • DOI: 10.1007/s10404-012-1102-8
  • Peer Reviewed
• [Journal Article] Open-tubular Electrochromatographic Chiral Separation of Amino Acids Using an Organic Nanocrystals Immobilized Capillary (2013)
  • Author(s): 北川文彦, 須田木裕, 末吉健志, 大塚浩二
  • Journal Title: Analytical Sciences Volume: 29 Issue: 1 Pages: 107-112
  • DOI: 10.2116/analsci.29.107
  • NAID: 10031147335
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed
• [Journal Article] Effect of a Low-conductivity Zone on Field-amplified Sample Stacking in Microchip Micellar Electrokinetic Chromatography (2013)
  • Author(s): 末吉健志, 北川文彦, 大塚浩二
  • Journal Title: Analytical Sciences Volume: 29 Issue: 1 Pages: 133-138
  • DOI: 10.2116/analsci.29.133
  • NAID: 10031147339
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed
• [Journal Article] Towards 10,000-fold Sensitivity Improvement of Oligosaccharides in Capillary Electrophoresis Using Large-volume Sample Stacking with an Electroosmotic Flow Pump Combined with Field-amplified (2013)
  • Author(s): 川井隆之, 上田真澄, 福島雄大, 末吉健志, 北川文彦, 大塚浩二
  • Journal Title: Electrophoresis Volume: (印刷中) Issue: 16 Pages: 2303-2310
  • DOI: 10.1002/elps.201200615
  • Peer Reviewed
• [Journal Article] Electrophoretic analysis of cations using large-volume sample stacking with an electroosmotic flow pump using capillaries coated with neutral and cationic polymers (2012)
  • Author(s): 川井隆之, 伊藤潤, 末吉健志, 北川文彦, 大塚浩二
  • Journal Title: J. Chromatogr. A Volume: 1267 Pages: 65-73
  • DOI: 10.1016/j.chroma.2012.09.077
  • Peer Reviewed
• [Journal Article] Sensitive enantioseparation by transient trapping-cyclodextrin electrokinetic chromatography (2012)
  • Author(s): 末吉健志, 戀野寛嗣, 北川文彦, 大塚浩二
  • Journal Title: J. Chromatogr. A Volume: 1,269 Pages: 366-371
  • DOI: 10.1016/j.chroma.2012.08.065
  • Peer Reviewed
• [Presentation] Strategies for Improving Analytical Performance in Microscale Electrophoresis (2014)
  • Author(s): Toyohiro Naito, Takuya Kubo, Koji Otsuka
  • Organizer: 4th International Conference on Microfluidic Chip and Micro/NanoScale Bioseparation Analysis
  • Place of Presentation: Wuhan, China
  • Year and Date: 2014-10-30 – 2014-11-02
  • Invited
• [Presentation] アフィニティリガンド内包ヒドロゲル部分充填キャピラリーを用いる電気泳動分析 (2014)
  • Author(s): 大塚浩二
  • Organizer: 第65回日本電気泳動学会総会
  • Place of Presentation: 橫浜情報文化センター（橫浜市）
  • Year and Date: 2014-10-24 – 2014-10-25
  • Invited
• [Presentation] ミクロスケール電気泳動の高機能化 (2014)
  • Author(s): 内藤豊裕, 末吉健志, 久保拓也, 北川文彦, 大塚浩二
  • Organizer: 日本分析化学会第63年会
  • Place of Presentation: 広島大学（東広島市, 広島）
  • Year and Date: 2014-09-17 – 2014-09-19
  • Invited
• [Presentation] Novel Separation Media for Microscale Electrophoresis and Liquid Chromatography (2014)
  • Author(s): Koji Otsuka
  • Organizer: 30th International Symposium on Chromatography (ISC2014)
  • Place of Presentation: Salzburg, Austria
  • Year and Date: 2014-09-14 – 2014-09-18
  • Invited
• [Presentation] Development of C60-Fullerene Bonded Capillaries for LC (2014)
  • Author(s): Yoshiki Murakami, Toyohiro Naito, Takuya Kubo, Koji Otsuka
  • Organizer: 2014 China-Japan-Korea Symposium on Analytical Chemistry (CJK2014)
  • Place of Presentation: Shenyang, China
  • Year and Date: 2014-08-22 – 2014-08-25
  • Invited
• [Presentation] Development of Novel Separation Media for Microscale Analysis (2014)
  • Author(s): Kenta Kuroda, Yoshiki Murakami, Toyohiro Naito, Takuya Kubo, Koji Otsuka
  • Organizer: 38th International Symposium on Capillary Chromatography (ISCC2014)
  • Place of Presentation: Riva del Garda, Italy
  • Year and Date: 2014-05-18 – 2014-05-23
  • Invited
• [Presentation] Novel Separation Media and Materials for Microscale Separations (2014)
  • Author(s): Kyosuke Nii, Yoshiki Murakami, Toyohiro Naito, Kenji Sueyoshi, Takuya Kubo, Madoka Takai, Koji Otsuka
  • Organizer: 41st International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC2014 New Orleans)
  • Place of Presentation: New Orleans, LO, USA
  • Year and Date: 2014-05-11 – 2014-05-15
  • Invited
• [Presentation] Strategies for Improving Detectability of Capillary Electrophoresis (2014)
  • Author(s): Koichi Kanemori, Hiroya Ota, Takayuki Kawai, Toyohiro Naito, Takuya Kubo, Koji Otsuka
  • Organizer: 30th International Symposium on Microscale Bioseparations (MSB2014)
  • Place of Presentation: Pecs, Hungary
  • Year and Date: 2014-04-27 – 2014-05-01
  • Invited
• [Presentation] Strategies for Improving Analytical Performance of Microscale Electrophoresis (2014)
  • Author(s): Yudai Fukushima, Koichi Kanemori, Toyohiro Naito, Takuya Kubo, Koji Otsuka
  • Organizer: PITTCON 2014
  • Place of Presentation: McCormick Place, Chicago, IL, USA
• [Presentation] オンサイト分析用携帯LCデバイスに用いるモノリス材料に関する基礎検討 (2014)
  • Author(s): 國澤研大, 内藤豊裕, 久保拓也, 大塚浩二
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋大学東山キャンパス, 名古屋
• [Presentation] フラーレン修飾型シリカモノリスキャピラリーカラムの開発 (2014)
  • Author(s): 村上祥規, 内藤豊裕, 久保拓也, 大塚浩二
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋大学東山キャンパス, 名古屋
• [Presentation] Towards highly sensitive detection of biogenic compounds in microscale electrophoresis ustng on-line sample concentration (2013)
  • Author(s): 大塚浩二
  • Organizer: PITTCON 2013
  • Place of Presentation: ペンシルベニアコンベンションセンター(Philadelphia, PA、USA)
  • Year and Date: 2013-03-21
• [Presentation] Combination of on-line sample concentration and mass spectrometric detection in microscale electrophoresis (2013)
  • Author(s): Hiroshi Koino, Hiroya Ota, Mami Oketani, Takayuki Kawai, Kenji Sueyoshi, Takuya Kubo, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: 37th International Symposium on Capillary Chromatography (ISCC2013)
  • Place of Presentation: Palm Springs, CA, USA
  • Invited
• [Presentation] 残留医薬品の分析前処理を目的とした分子インプリントポリマーの開発 (2013)
  • Author(s): 久保拓也, 黒田健太, 細矢 憲, 大塚浩二
  • Organizer: 第73回分析化学討論会
  • Place of Presentation: 北海道大学函館キャンパス, 函館
• [Presentation] バイオマスナノファイバーを利用する親水性相互作用動電クロマトグラフィーの開発 (5) (2013)
  • Author(s): 渡邉正登, 川井隆之, 末吉健志, 久保拓也, 北川文彦, 大塚浩二
  • Organizer: 第73回分析化学討論会
  • Place of Presentation: 北海道大学函館キャンパス, 函館
• [Presentation] ナノ炭素材料固定化技術の開発とマイクロ分析への応用 (2013)
  • Author(s): 久保拓也, 冨永雄一, 村上祥規, 大塚浩二
  • Organizer: 日本材料学会第62期学術講演会
  • Place of Presentation: 東京工業大学大岡山キャンパス, 東京
• [Presentation] 音を利用したマイクロ流体デバイスへの小型電力供給システム (2013)
  • Author(s): 内藤豊裕, 加地範匡, Severine Le Gac, 安井隆雄, 渡慶次学, Albert van den Berg, 久保拓也, 大塚浩二, 馬場嘉信
  • Organizer: 化学とマイクロ・ナノシステム学会第27回研究会
  • Place of Presentation: 東北大学・片平キャンパスさくらホール, 仙台
• [Presentation] オンラインSPE-LC-MSを用いた環境中残留医薬品の高選択／高感度分析 (2013)
  • Author(s): 久保拓也, 黒田健太, 細矢 憲, 大塚浩二
  • Organizer: 第20回クロマトグラフィーシンポジウム
  • Place of Presentation: 神戸大学百年記念館, 神戸
• [Presentation] ナノ炭素材料の固定化と新規分離場開発への応用 (2013)
  • Author(s): 久保拓也, 大塚浩二
  • Organizer: JSPS創造機能化学第116委員会6月期合同分科会
  • Place of Presentation: 学士会館, 東京
• [Presentation] Towards high-sensitive detection in microscale electrophoresis using on-line sample concentration (2013)
  • Author(s): Koji Otsuka, Hiroshi Koino, Saeko Kinami, Hiroya Ota, Takayuki Kawai, Kenji Sueyoshi, Takuya Kubo, Fumihiko Kitagawa
  • Organizer: 39th International Symposium on Liquid-Phase Separations and Related Techniques (HPLC2013 Amsterdam)
  • Place of Presentation: Amsterdam, The Netherlands
  • Invited
• [Presentation] Novel separation media in microscale electrophoresis for selective analysis (2013)
  • Author(s): Yudai Fukushima, Masahiro Ikawa, Masato Watanabe, Takayuki Kawai, Kenji Sueyoshi, Takuya Kubo, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: 9th Balaton Symposium
  • Place of Presentation: Siofok, Hungary
  • Invited
• [Presentation] アフィニティリガンド内包ヒドロゲル部分充填キャピラリーを用いる電気泳動分析(5) (2013)
  • Author(s): 福島雄大, 内藤豊裕, 末吉健志, 久保拓也, 大塚浩二
  • Organizer: 日本分析化学会第62年会
  • Place of Presentation: 近畿大学東大阪キャンパス, 東大阪
• [Presentation] High Performance Microscale Electrophoresis for Bioanalysis (2013)
  • Author(s): Fumihiko Kitagawa, Koji Otsuka
  • Organizer: International Symposium on Interdisciplinary Research and Education on Medical Device Developments (IREMD2013)
  • Place of Presentation: 弘前大学, 弘前
  • Invited
• [Presentation] Towards highly selective and sensitive analysis in microscale electrophoresis (2013)
  • Author(s): Yudai Fukushima, Koichi Kanemori, Kenji Sueyoshi, Takuya Kubo, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: 20th International Symposium on Electro- and Liquid Phase-Separation Techniques (ITP2013)
  • Place of Presentation: Puerto de la Cruz, Tenerife, Canary Islands, Spain
  • Invited
• [Presentation] 高選択性分離剤を用いたオンラインSPE-LC-MSによる河川水中スルピリドの高感度分析 (2) (2013)
  • Author(s): 黒田健太, 内藤豊裕, 久保拓也, 大塚浩二
  • Organizer: 第3回CSJ化学フェスタ2013
  • Place of Presentation: タワーホール船堀, 東京
• [Presentation] Development of Microfluidic Blotting Devices Using Alginate Hydrogel (2013)
  • Author(s): Yudai Fukushima, Toyohiro Naito, Kenji Sueyoshi, Takuya Kubo, Koji Otsuka
  • Organizer: 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)
  • Place of Presentation: Messe Freiburg, Freiburg, Germany
• [Presentation] Development of Electrospray Ionization Interface-Integrated Microchip for Mass Spectrometric Detection in Electrophoresis (2013)
  • Author(s): Mami Oketani, Takayuki Kawai, Toyohiro Naito, Kenji Sueyoshi, Takuya Kubo, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)
  • Place of Presentation: Messe Freiburg, Freiburg, Germany
• [Presentation] Development of Novel Separation Media for LC and SPE: C60-fullerene Coated Capillary and Molecularly Imprinted Adsorbent (2013)
  • Author(s): Yoshiki Murakami, Kenta Kuroda, Toyohiro Naito, Takuya Kubo, Koji Otsuka
  • Organizer: 13th Asia Pacific Symposium on Microscale Separations and Analysis (APCE2013)
  • Place of Presentation: Lotte Hotel Jeju, Jeju Island, Korea
  • Invited
• [Presentation] LVSEP-CE-MSによる糖鎖分析の高感度化 (2013)
  • Author(s): 太田尋也, 川井隆之, 内藤豊裕, 末吉健志, 久保拓也, 北川文彦, 大塚浩二
  • Organizer: 第24回クロマトグラフィー科学会議
  • Place of Presentation: 東京大学
• [Presentation] キノリンボロン酸との錯形成を利用した糖のラベルフリー電気泳動分析 (5) (2013)
  • Author(s): 兼森紘一, 川井隆之, 内藤豊裕, 末吉健志, 久保拓也, 北川文彦, 大塚浩二
  • Organizer: 第33回キャピラリー電気泳動シンポジウム (SCE2013)
  • Place of Presentation: 日本女子大学, 東京
• [Presentation] Strategies for improving analytical performance of microscale electrophoresis (2013)
  • Author(s): Hiroshi Koino, Yudai Fukushima, Hiroya Ota, Koichi Kanemori, Takayuki Kawai, Kenji Sueyoshi, Takuya Kubo, Fumihiko Kitagawa, Koji Otsuka
  • Organizer: 40th International Symposium on Liquid-Phase Separations and Related Techniques (HPLC2013 Hobart)
  • Place of Presentation: Hobart, Tasmania, Australia
  • Invited
• [Presentation] ナノ炭素材料の固定化に関する基礎検討 (2013)
  • Author(s): 久保拓也, 大塚浩二
  • Organizer: 第57回日本学術会議材料工学連合講演会
  • Place of Presentation: 京都テルサ, 京都
• [Presentation] Controllable Swelling/Shrinking Behavior of Hydrogels by Molecular Adsorptions, (2013)
  • Author(s): Takuya Kubo, Koji Otsuka
  • Organizer: 2013 MRS Fall Meeting & Exhibit
  • Place of Presentation: Boston, MA, USA
• [Presentation] 高横縦比六角柱型マイクロピラーのLCカラムへの応用 (2013)
  • Author(s): 二上俊太, Jeff Op De Beeck, 内藤豊裕, 久保拓也, 大塚浩二, Han Gardeniers, Gert Desmet, Wim De Malsche
  • Organizer: 化学とマイクロ・ナノシステム学会第28回研究会
  • Place of Presentation: イーグレ姫路, 姫路
• [Presentation] Strategies for high-sensitive detection in microscale electrophoresis (2012)
  • Author(s): 大塚浩二
  • Organizer: 12th Asia-Pacific International Symposium on Microscale Separation and Analysis (APCE2012)
  • Place of Presentation: シンガポール国立大学(シンガポール)(招待講演)
  • Year and Date: 2012-12-17
• [Presentation] Strategies for improving detectability in microscale electrophoresis (2012)
  • Author(s): 大塚浩二
  • Organizer: 19th International Symposium on Electro- and Liquid Phase-separation Techniques OTP2012)
  • Place of Presentation: Baltimore, MD, USA(招待講演)
  • Year and Date: 2012-10-02
• [Presentation] ESI interface-integrated microdevice for an application of LVSEP to microchip electrophoresi-mass spectrometry (2012)
  • Author(s): 桶谷満美
  • Organizer: 19th International Symposium on Electro- and Liquid Phase-separation Techniques (ITP 2012)
  • Place of Presentation: Baltimore, MD, USA
  • Year and Date: 2012-10-02
• [Presentation] トランジェント-トラッピング法によるミクロスケール電気泳動分析の高感度化(7) (2012)
  • Author(s): 戀野寛嗣
  • Organizer: 日本分析化学会第61年会
  • Place of Presentation: 金沢大学角間キャンパス
  • Year and Date: 2012-09-19
• [Presentation] 電気泳動分析用アフィニティリガンド内包ヒドロゲル部分充填キャピラリーの開発(4) (2012)
  • Author(s): 福島雄大
  • Organizer: 日本分析化学会第61年会
  • Place of Presentation: 金沢大学角間キャンパス
  • Year and Date: 2012-09-19
• [Presentation] LVSEP法の適用によるキャピラリー電気泳動-質量分析法の高感度化(2) (2012)
  • Author(s): 太田尋也
  • Organizer: 日本分析化学会第61年会
  • Place of Presentation: 金沢大学角間キャンパス
  • Year and Date: 2012-09-19
• [Presentation] Highly sensitive electrophoretic analysis using LVSEP : Application to cationic analytes. 2 (2012)
  • Author(s): 伊藤潤
  • Organizer: 2012 RSC Tokyo International Conference, JASIS Conference
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2012-09-06
• [Presentation] Chitin-modified poly(dimethylsiloxane) microchannel for electrophoretic analysis of biogenic compounds. 2 (2012)
  • Author(s): 堀祐輔
  • Organizer: 2012 RSC Tokyo International Conference, JASIS Conference
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2012-09-06
• [Presentation] Development ofhydrophilic interaction electrokinetic chromatography using chitin/chitosan nanofibers. 3 (2012)
  • Author(s): 渡邉正登
  • Organizer: 2012 RSC Tokyo International Conference, JASIS Conference
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2012-09-06
• [Presentation] Strategies for improving sensitivity in microscale eleetrophoretic analysis of biogenic compounds using on-line sample concentration (2012)
  • Author(s): 大塚浩二
  • Organizer: 38th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC2012)
  • Place of Presentation: Anaheim, CA, USA(招待講演)
  • Year and Date: 2012-06-19
• [Presentation] Strategies for high-sensitive detection in microscale electrophoresis (2012)
  • Author(s): 大塚浩二
  • Organizer: 28th International Symposium on Microscale Bioseparations (MSB2012Shanghai)
  • Place of Presentation: 上海(中国)(招待講演)
• [Presentation] キャピラリー電気泳動－質量分析法の高感度化・高性能化
  • Author(s): 久保拓也, 大塚浩二
  • Organizer: 京都バイオ計測センター研究発表会
  • Place of Presentation: 京都高度技術研究所, 京都
• [Presentation] 分子間相互作用を利用したヒドロゲルの開発
  • Author(s): 久保拓也, 大塚浩二
  • Organizer: JSPS創造機能化学第116委員会1月期合同分科会
  • Place of Presentation: 学士会館, 東京