| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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| Outline of Final Research Achievements |
Understanding of freezing characteristics of mature nerve cells is important from the viewpoint of application: cryosurgery and cryopreservation, but also fundamentally because of the unique morphology of the nerve cells with neurites. Microscopic behaviors of freezing and thawing of the mature nerve cells were investigated. Beading and shortening of neurites after freezing and thawing were found and such morphological changes were quantified under various freezing conditions. Next, the effect of a common cryoprotectant, 10v/v% dimethyl sulfoxide (DMSO), on the freezing and thawing behaviors of the cells was investigated. The results show that DMSO largely reduced such morphological change of neurites and largely improved the post-thaw cell viability.
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