Fundamental Characteristics of Freezing of Mature Nerve Cells Related to Cryosurgery and Cryopreservation
Project/Area Number |
24360083
|
Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Thermal engineering
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Research Institution | Kyushu Institute of Technology |
Principal Investigator |
ISHIGURO HIROSHI 九州工業大学, 生命体工学研究科(研究院), 教授 (30176177)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
|
Keywords | 熱工学 / 生体熱工学 / 凍結手術 / 凍結保存 / 成熟神経細胞 / 凍結融解挙動 / 生存性 / 形態変化 / 凍結・融解挙動 |
Outline of Final Research Achievements |
Understanding of freezing characteristics of mature nerve cells is important from the viewpoint of application: cryosurgery and cryopreservation, but also fundamentally because of the unique morphology of the nerve cells with neurites. Microscopic behaviors of freezing and thawing of the mature nerve cells were investigated. Beading and shortening of neurites after freezing and thawing were found and such morphological changes were quantified under various freezing conditions. Next, the effect of a common cryoprotectant, 10v/v% dimethyl sulfoxide (DMSO), on the freezing and thawing behaviors of the cells was investigated. The results show that DMSO largely reduced such morphological change of neurites and largely improved the post-thaw cell viability.
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Report
(4 results)
Research Products
(26 results)