In vitro assay system for evaluating curative effect of myoblast sheet transplantation
| Project/Area Number |
24360341
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Osaka University |
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Principal Investigator |
KINO-OKA Masahiro 大阪大学, 工学(系)研究科(研究院), 教授 (40234314)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2012: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Keywords | 細胞シート / 筋芽細胞 / 血管新生 / 細胞アッセイ / 流動性 / 再生医療 / 培養組織 / 創薬スクリーニング / シミュレーション / 骨格筋筋芽細胞 / 間葉系幹細胞 / ガン細胞 / 薬剤スクリーニング / シミュレータ |
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| Outline of Final Research Achievements |
Autologous transplantation of myoblast sheet has attracted attention as a new technique for curing myocardial infarction. Myoblast sheet has the ability to secret cytokines which improve heart function via the facilitation of angiogenesis on affected part. To understand in vivo phenomena and evaluate curative effect after transplantation such as cytokine secretion from transplant and its angiogenesis capability, a multi-layered cell sheet of human skeletal muscle myoblasts (HSMMs) was constructed by originally equipped gelatin stamp system. In the HSMM sheet, active cellular migration occurred in the horizontal and vertical directions anywhere, revealing the sheet fluidity. Dynamic behavior including collective migration and spatial habitation of heterogeneous cells, such as human skeletal muscle fibroblasts (HSMFs) and human umbilical vein endothelial cells (HUVECs), in the fluidic scaffold was clarified.
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Report
(4 results)
Research Products
(34 results)
