Molecular mechanism of the formation and transfer of functional unit of LINE
| Project/Area Number |
24370001
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Keywords | レトロトランスポゾン / 転移機構 / LINE / 核移行 / リボヌクレオタンパク質 / 核小体 / rDNA / テロメア |
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| Outline of Final Research Achievements |
Non-LTR retrotransposon, so called Long Interspersed Nuclear Elements (LINE), is moved actively in most eukaryotic cells, but many processes involved in its transposition is largely unknown. In this study, we analyzed the regulation mechanisms of translation of open reading frame 1 protein (ORF1p) and open reading frame 2 protein (ORF2p) using human L1 element. In addition, we here revealed how ORF1p and ORF2p form the functional ribonucleoprotein (RNP) complex, and moves into the nucleoli, using rDNA specific LINE (R elements), R1, R2 and R7. Furthermore, we have clarified molecular mechanisms underlying how the LINE unit interacts and digests the target sequence correctly, and how the LINE mRNA binds to the target site, and reverse transcribe occurs from the correct site, using the R7 element.
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Report
(5 results)
Research Products
(73 results)
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[Journal Article] Identification of two juvenile hormone inducible transcription factors from the silkworm, Bombyx mori2015
Author(s)
Hitoshi Matsumoto, Chihiro Ueno, Yuki Nakamura, Terunori Kinjoh, Yuka Ito, Sachiko Shimura, Hiroaki Noda, Shigeo Imanishi, Kazuei Mita, Fujiwara Haruhiko, Kiyoshi Hiruma, Tetsuro Shinoda, and Manabu Kamimura
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Journal Title
J. Insect Physiol.
Volume: なし
Pages: 31-41
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Molecular Basis of the wing coloration in a Batesian mimic butterfly, Papilio polytes.2013
Author(s)
Nishikawa, H., Iga, M., Yamaguchi, J., Saito, K., Kataoka, H., Suzuki, Y., Sugano, S. and Fujiwara, H.
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Journal Title
Sci. Rep.
Volume: 3, e3184
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Large Scale Full-Length cDNA Sequencing Reveals a Unique Genomic Landscape in a Lepidopteran Model Insect, Bombyx mori.2013
Author(s)
Suetsugu Y, Futahashi R, Kanamori H, Kadono-Okuda K, Sasanuma SI, Narukawa J, Ajimura M, Jouraku A, Namiki N, Shimomura M, Sezutsu H, Osanai-Futahashi M, Suzuki MG, Daimon T,
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Journal Title
G3
Volume: 3
Pages: 1481-1492
DOI
Related Report
Peer Reviewed
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[Journal Article] Two adjacent cis-regulatory elements are required for ecdysone response of ecdysone receptor (EcR) B1 transcription.2012
Author(s)
Shirai,H., Kamimura,M., Yamaguchi,J., Imanishi,S., Kojima,T. and Fujiwara, H.
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Journal Title
PLoS One
Volume: 7(11) e:49348.
DOI
Related Report
Peer Reviewed
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[Presentation] 擬態の不思議な世界2014
Author(s)
藤原晴彦
Organizer
日本皮膚科学会・中部支部学術大会
Place of Presentation
グランフロント大阪（大阪府）
Year and Date
2014-10-25
Related Report
Invited
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[Presentation] The Papilio genome project to understand various types of protective mimicry.2013
Author(s)
Fujiwara H., Yamaguchi J., Ando T., Ijima T., Hori H., Toyoda A., Ito T., Hirakawa H., Kosugi S., Fujiyama A., Suzuki Y., Sugano S., Nishikawa H., Futahashi R. and Shirataki H.
Organizer
International Symposium on Genome Science, Expanding Frontiers of Genome Science
Place of Presentation
東京大学・本郷キャンパス
Year and Date
2013-01-09
Related Report
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[Presentation] Retrotransposition mechanisms and application of sequence-specific non-LTR retrotransposon R201 in vertebrate.2012
Author(s)
Fujiwara, H., Kuroki A., Mizuno, S., Taefusa, J., Seto, Y., Yatabe, H. and Kawashima, T.
Organizer
Fujihara seminar 2012, A new horizon of retroposon research
Place of Presentation
芝蘭会館・稲盛ホール(京都市)(招待講演)
Year and Date
2012-08-03
Related Report
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