|Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
|Outline of Final Research Achievements
We have been studying electron transfer between Fd and these enzymes by X-ray crystallography, NMR analysis and isothermal calorimetry to obtain an insight into electron partitioning in chloroplasts. In all cases, their protein-protein interactions are not so strong with Kd values around micro-molar to several tens micro-molar; a set of positively charged residues of the partner enzymes is closed to acidic residues of Fd in a spatially matching manner to produce an electrostatic interaction force. Whole acidic surface of Fd is not utilized for complex formation with partner enzymes and the interaction region appears to be at least partly unique for each enzyme. This topological variety of the molecular recognition between Fd and partner enzymes would be significant for function of Fd as a hub protein in a network of redox metabolisms.We further found the intermolecular interaction of some Fd-dependent enzymes with Fd varied when each enzyme was bound to their substrate.