Mechanisms regulating early embryogenesis in Arabidopsis thaliana

• Project/Area Number: 24370022
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Nakajima Keiji 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (80273853)
• Research Collaborator: KIM Jong-Myong ; ENDO Takaho ; KOI Satoshi ; HISANAGA Tetsuya ; FURUTA Kaori ; KOHCHI Takayuki ; SHIMAMURA Masaki ; YAMATO Katsuyuki ; ISHIZAKI Kimitsune ; LUKOWITZ Wolfgang
• Project Period (FY): 2012-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: 胚発生 / パターン形成 / 植物 / シロイヌナズナ / 発生・分化 / 卵 / 初期化 / 生殖細胞 / リプログラミング / シグナル伝達 / 発現制御 / 発生分化 / 発生制御

Outline of Final Research Achievements

Mechanisms regulating early phases of plant embryogenesis are poorly understood. We have previously identified a member of the plant-specific RKD transcription factors, RKD4, as a regulator of early embryogenesis in Arabidopsis thaliana. The present study revealed that RKD4 directly binds to the genomic DNAs of many genes including known regulators of embryogenesis, and thereby regulates their expression. In addition, genetic studies using liverworts and Arabidopsis indicated that RKD factors are evolutionarily conserved regulators of germ cell differentiation in land plants.

Research Products

• [Journal Article] An evolutionary conserved plant RKD factor controls germ cell differentiation. (2016)
  • Author(s): Koi, S., Hisanaga, T., Sato, K., Shimamura, M., Yamato, K.T., Ishizaki, K., Kohchi, T. and Nakajima, K.
  • Journal Title: Current Biology Volume: 26 Issue: 13 Pages: 1775-1781
  • DOI: 10.1016/j.cub.2016.05.013
  • Peer Reviewed / Open Access
• [Journal Article] The Naming of Names: Guidelines for Gene Nomenclature in Marchantia (2016)
  • Author(s): Bowman, J., Araki, T., Arteaga-Vazquez, M., Berger, F., Dolan, L., Haseloff, J., Ishizaki, K., Kyozuka, J., Lin, S.S., Nagasaki, H., Nakagami, H., Nakajima, K., Nakamura, Y., Ohashi-Ito, K., Sawa, S., Shimamura, M., Solano, R., Tsukaya, H., Ueda, T., Watanabe, Y., Yamato, K.T., Zachgo, S., and Kohchi, T.
  • Journal Title: Plnat cell Physiology Volume: 57 Issue: 2 Pages: 257-261
  • DOI: 10.1093/pcp/pcv193
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 植物細胞を初期化するRKD遺伝子の発見 (2013)
  • Author(s): 中島 敬二
  • Journal Title: 化学と生物 Volume: 58 Pages: 789-791
• [Journal Article] A GAL4-based targeted activation tagging system in Arabidopsis (2013)
  • Author(s): Waki, T., Miyashima, S., Nakanishi, M., Ikeda, Y., Hashimoto, T., Nakaiima, K.
  • Journal Title: Plant J. Volume: 73 Issue: 3 Pages: 357-367
  • DOI: 10.1111/tpj.12049
  • Peer Reviewed
• [Journal Article] Peptide Separation Methodologies for in-depth Proteomics in Arabidopsis. (2013)
  • Author(s): Fukao, Y, Yoshida, M, Kurata, R, Kobayashi, M, Nakanishi, M, Fujiwara, M, Nakajima, K., and Feriani, A.
  • Journal Title: Plant Cell Physiol. Volume: (in press) Issue: 5 Pages: 808-815
  • DOI: 10.1093/pcp/pct033
  • NAID: 10031177057
  • Peer Reviewed
• [Presentation] MpFGMYBはゼニゴケにおいて生殖器官の雌性化に機能する (2017)
  • Author(s): 久永哲也、岡橋啓太郎、山岡尚平、西浜竜一、河内孝之、中島敬二
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（鹿児島県・鹿児島市）
  • Year and Date: 2017-03-17
• [Presentation] Marchantia polymorpha as a model to study plant reproductive development (2016)
  • Author(s): Tetsuya Hisanaga, Satoshi Koi, Katsutoshi Sato, Keiji Nakajima
  • Organizer: Marchantia training course
  • Place of Presentation: 京都大学（京都府・京都市）
  • Year and Date: 2016-12-12
  • Invited
• [Presentation] A MYB type transcription factor controls female reproductive organ development in Marchantia polymorpha (2016)
  • Author(s): Testuya Hisanaga, Keiji Nakajima
  • Organizer: EMBO work shop ”New model systems for early land plant evolution”
  • Place of Presentation: ウィーン（オーストリア）
  • Year and Date: 2016-06-23
  • Int'l Joint Research
• [Presentation] Marchantia polymorpha as a genetic tool to study plant egg cell differentiation (2016)
  • Author(s): Keiji Nakajima
  • Organizer: Marchantia Seminars: Welcome to Marchantia world!
  • Place of Presentation: 京都大学 （京都府京都市）
  • Year and Date: 2016-03-03
  • Int'l Joint Research / Invited
• [Presentation] Conservation and diversification of reprogramming factors in land plant evolution (2015)
  • Author(s): Satoshi Koi, Tetsuya Hisanaga, Gaku Fukui, Kaori Furuta, Masaki Shimamura, Takafumi Watabe, Katsuyuki Yamato, Kimitsune Ishizaki, Takayuki Kohchi, Keiji Nakajima
  • Organizer: International Symposium on Plant Sciences & Annual Conference of the Korean Society of Plant Biologists
  • Place of Presentation: Chungnam National University (Daejeon Korea)
  • Year and Date: 2015-11-06
  • Int'l Joint Research / Invited
• [Presentation] RKD genes are conserved regulator for egg cell differentiation among land plants (2015)
  • Author(s): Tetsuya Hisanaga, Satoshi Koi, Gaku Fukui, Keiji Nakajima
  • Organizer: International ERATO Higashiyama Live-Holonics Symposium and Technical Workshop 2015
  • Place of Presentation: 名古屋大学 （愛知県名古屋市）
  • Year and Date: 2015-08-27
  • Int'l Joint Research
• [Presentation] Control of germ cell differentiation and pluripotency by evolutionarily conserved factors in land plants (2015)
  • Author(s): Satoshi Koi, Tetsuya Hisanaga, Gaku Fukui, Kaori Furuta, Masaki Shimamura,Takafumi Watabe, Katsuyuki Yamato, Kimitsune Ishizaki, Takayuki Kohchi, Keiji Nakajima
  • Organizer: International ERATO Higashiyama Live-Holonics Symposium and Technical Workshop 2015
  • Place of Presentation: 名古屋大学 （愛知県名古屋市）
  • Year and Date: 2015-08-27
  • Int'l Joint Research / Invited
• [Presentation] ゼニゴケの生殖におけるRWP-RKファミリー遺伝子MpRKDの機能 (2015)
  • Author(s): 厚井聡、久永哲也、嶋村正樹、石崎公庸、河内孝之、中島 敬二
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都世田谷区）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] An enhancer trap screening to identify genes regulating egg cell specification and differentiation in Marchantia polymorpha (2015)
  • Author(s): Tetsuya Hisanaga, Keiji Nakajima
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都世田谷区）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Comparative studies of female gametogenesis in land plants (2014)
  • Author(s): Keiji Nakajima
  • Organizer: Marchantia Workshop 2014
  • Place of Presentation: 神戸大学（兵庫県神戸市）
  • Year and Date: 2014-12-08 – 2014-12-10
  • Invited
• [Presentation] Evolutionary Insights into the RKD Gene Function in the Development of Land Plants (2013)
  • Author(s): 厚井聡、石崎公庸、橋本隆、河内孝之、中島敬二
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2013-03-23
• [Presentation] モデル苔類ゼニゴケを用いた初期胚発生に関わる遺伝子の機能解析 (2013)
  • Author(s): 厚井聡、石崎公庸，河内孝之、中島敬二
  • Organizer: 日本蘚苔類学会第42 回大会
  • Place of Presentation: 岡山理科大学
• [Presentation] Evolutionarily conserved role of RKD gene family in land plant reproduction
  • Author(s): Satoshi Koi, Kimitsune Ishizaki, Takayuki Kohchi, Keiji Nakajima
  • Organizer: Marchatia IV
  • Place of Presentation: Melbourne
• [Remarks] 植物で生殖細胞をつくり出す主役の遺伝子を発見 ～生殖機構の解明や、繁殖・育種技術への応用に期待～
  • URL: http://www.naist.jp/pressrelease/2016/06/003257.html
• [Remarks] 植物で生殖細胞をつくり出す主役の遺伝子を発見 ～生殖機構の解明や、繁殖・育種技術への応用に期待～
  • URL: http://bsw3.naist.jp/research/index.php?id=1340
• [Remarks] 研究テーマ：生殖細胞が作られるしくみ
  • URL: http://bsw3.naist.jp/nakajima/Research_JP/Germ_cell.html
• [Remarks] 胚発生とリプログラミング
  • URL: http://bsw3.naist.jp/nakajima/Research_JP/embryogenesis.html
• [Remarks]
  • URL: http://bsw3.naist.jp/nakajima/