Molecular mechanisms in a visual transduction system of invertebrate rhodopsin
Project/Area Number |
24370043
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Structural biochemistry
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Research Institution | Nagoya University |
Principal Investigator |
Murakami Midori 名古屋大学, 理学(系)研究科(研究院), 助教 (20324387)
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Co-Investigator(Kenkyū-buntansha) |
Kouyama Tsutomu 名古屋大学, 大学院理学研究科, 教授 (30170210)
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
|
Keywords | ロドプシン / 光情報変換 / 結晶構造解析 / 光受容 / X線結晶構造解析 / 情報伝達 |
Outline of Final Research Achievements |
Blue light illumination at 100 K induced a red-shift of the absorption maximum, indicating that an equilibrium state of three isomeric states, Rhodopsin, Batho and 9-cis isorhodopsin was established. When this crystal was warmed to 240K, the absorption maximum was blue-shifted. Accurate estimation of isomer composition under this condition suggested that Batho was converted to LM with no change in the contents of the other states. We collected diffraction data set and calculate the difference electron density maps. It indicates that the conformational changes are located at the vicinity of the active site upon formation the LM intermediate. The larger movements of helices that characterize meta formation are not initiated at the early stage of photocycle in squid rhodopsin, but occur during the transition from LM to Meta. We try to crystallize the Acid Meta state of squid rhodopsin and succeed to obtain some new crystals which can diffract up to high resolution.
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Report
(5 results)
Research Products
(51 results)
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[Journal Article] Proteins2012
Author(s)
Zhang J.
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Journal Title
Crystal structure of the O intermediate of the Leu93→Ala mutant of bacteriorhodopsin
Volume: 80
Issue: 10
Pages: 2384-2396
DOI
Related Report
Peer Reviewed
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[Presentation] クラックスロドプシンの3量体構造と光安定性2014
Author(s)
S. K. Chan, T. Kitajima, M. Murakami, K. Ihara, T. Kouyama
Organizer
日本生物物理学会第52回年会
Place of Presentation
札幌
Year and Date
2014-09-25 – 2014-09-27
Related Report
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