Mechanistic analyses of chromatin structural changes through the structure of TFIID

• Project/Area Number: 24370050
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: High Energy Accelerator Research Organization
• Principal Investigator: SENDA Toshiya 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 教授 (30272868)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2012: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: X線結晶構造解析 / クロマチン / ヌクレオソーム / 転写 / TFIID / 精製 / 複合体 / 天然変性状態

Outline of Final Research Achievements

Nucleosomes are the fundamental repeating units of chromatin. To initiate the transcription reaction, histones must be removed from genomic DNA at gene coding regions. Previously, we determined the cocrystal structure of CIA and double bromodomain, and proposed a recruitment and disassembly model (hiMOST model). In this study, we established the methods for large-scale purification of the HAT domain of TAF1, and the conserved domain of TAF7. In order to perform functional analysis of TFIID in chromatin transcription, we also tried to reconstitute the complex of TBP and TFIIB on promoter DNA.

Research Products

• [Journal Article] Molecular mechanism of strict substrate specificity of an Extradiol Dioxygenase, DesB, derived from Sphingobium sp. SYK-6. (2014)
  • Author(s): Sugimoto, K., Senda, M., Kasai, D., Fukuda, M., Masai, E. and Senda, T.
  • Journal Title: Biochem. J. Volume: 9 Pages: 257-265
  • DOI: 10.1371/journal.pone.0092249
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] エピジェネティックおよび疾病関連タンパク質の構造生物学研究 (2014)
  • Author(s): 千田俊哉
  • Organizer: 日本結晶学会
  • Place of Presentation: 東京・東京大学農学部キャンパス
  • Year and Date: 2014-11-01
  • Invited
• [Presentation] Large-scale purification of general transcription factor TFIIF (2014)
  • Author(s): Naruhiko Adachi, Kyohei Aizawa, Yuka Yamaguchi, and Toshiya Senda
  • Organizer: Epigenetics & chromatin
  • Place of Presentation: 米国・コールドスプリングハーバー研究所
  • Year and Date: 2014-09-09 – 2014-09-13
• [Presentation] Purification and crystallization of TAF subunits of general transcription initiation factor TFIID (2013)
  • Author(s): 安達成彦
  • Organizer: 名古屋シンポジウム
  • Place of Presentation: 名古屋大学細胞生理学研究センター
  • Year and Date: 2013-01-22
• [Presentation] 転写基本因子TFIIDのユビキチン活性化/結合酵素活性制御ドメインの結晶化に向けた精製 (2013)
  • Author(s): 相沢恭平・安達成彦・千田俊哉
  • Organizer: 第86回 日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] 転写基本因子TFIIDを中心とした転写活性化の分子機構の解析 (2012)
  • Author(s): 安達成彦
  • Organizer: 第12回日本蛋白質科学会年会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2012-06-22
• [Presentation] 構造生物学の将来と放射光
  • Author(s): 千田俊哉
  • Organizer: 日本放射光学会
  • Place of Presentation: 広島
  • Invited
• [Book] Encyclopedia of Systems Biology "Histone posttranslational modification to nucleosome structural change."
  • Author(s): 安達成彦、千田俊哉
  • Publisher: Springer
• [Remarks] KEK, IMSS, SBRC
  • URL: http://pfweis.kek.jp/index.html