• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Single-molecule analysis of 1-nm stepping motion of linear motor carbohydrate hydrolase

Research Project

Project/Area Number 24370062
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Biophysics
Research InstitutionOkazaki Research Facilities, National Institutes of Natural Sciences (2014)
The University of Tokyo (2012-2013)

Principal Investigator

IINO RYOTA  大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (70403003)

Co-Investigator(Kenkyū-buntansha) UCHIHASHI Takayuki  金沢大学, 数物科学系, 准教授 (30326300)
IGARASHI Kiyohiko  東京大学, 農学生命科学研究科, 准教授 (80345181)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Keywords分子モーター / 1分子計測 / セルラーゼ / キチナーゼ / 1分子計測・操作 / モータータンパク質
Outline of Final Research Achievements

In this study, using single-molecule fluorescence microscopy and high speed atomic force microscopy, we determined kinetic constants of the elementary reaction steps for carbohydrate hydrolase TrCel7A against cellulose Iα and IIII. TrCel7A displayed similar binding and dissociation rate constants for cellulose Iα and IIII and similar fractions of productive binding on cellulose Iα and IIII. Furthermore, once productively bound, TrCel7A processively hydrolyzes and moves along cellulose Iα and IIII with similar translational rates. With structural models of cellulose Iα and IIII, we propose that different susceptibilities at high TrCel7A concentration arise from surface properties of substrate, including ratio of hydrophobic surface and number of available lanes.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (26 results)

All 2015 2014 2013 2012

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 4 results) Presentation (15 results) (of which Invited: 10 results) Book (2 results)

  • [Journal Article] Single-molecule Imaging Analysis of Elementary Reaction Steps of Trichoderma reesei Cellobiohydrolase I (Cel7A) Hydrolyzing Crystalline Cellulose Iα and IIII2014

    • Author(s)
      Y.Shibafuji, A.Nakamura, T.Uchihashi, N.Sugimoto, S.Fukuda, H.Watanabe, M.Samejima, T.Ando, H.Noji, A.Koivula, K.Igarashi, R.Iino
    • Journal Title

      Journal of Biological Chemistry

      Volume: 289 (29) Issue: 20 Pages: 1405614065-1405614065

    • DOI

      10.1074/jbc.m113.546085

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Torque generation of Enterococcus hirae V-ATPase2014

    • Author(s)
      Ueno H, Minagawa Y, Hara M, Rahman S, Yamato I, Muneyuki E, Murata T, and Iino R
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 289 Issue: 45 Pages: 31212-31223

    • DOI

      10.1074/jbc.m114.598177

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article]2014

    • Author(s)
      T.Ikeda, T.Tsukahara, R.Iino, M.Takeuchi, H.Noji
    • Journal Title

      Angew. Chem. Int. Ed.

      Volume: 53, (accepted) Issue: 38 Pages: 10082-10085

    • DOI

      10.1002/anie.201403091

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Real-Time Fluorescence Visualization of Slow Tautomerization of Single Free-Base Phthalocyanines under Ambient Conditions2014

    • Author(s)
      Ikeda T, Iino R and Noji H
    • Journal Title

      Chem. Commun

      Volume: 50 Issue: 67 Pages: 9443-9446

    • DOI

      10.1039/c4cc02574a

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular Mechanism of an Exo-type Cellulase Revealed by Single-molecule Analysis2014

    • Author(s)
      飯野亮太, 中村彰彦, 五十嵐圭日子, 鮫島正浩
    • Journal Title

      Seibutsu Butsuri

      Volume: 54 Issue: 6 Pages: 318-320

    • DOI

      10.2142/biophys.54.318

    • NAID

      130004713033

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] High-speed atomic force microscope combined with single-molecule fluorescence microscope.2013

    • Author(s)
      S. Fukuda, T. Uchihashi, R. Iino, Y. Okazaki, M. Yoshida, K. Igarashi, T. Ando
    • Journal Title

      Rev. Sci. Instrum.

      Volume: 84 Issue: 7 Pages: 73706-73706

    • DOI

      10.1063/1.4813280

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intersubunit coordination and cooperativity in ring-shaped NTPases2013

    • Author(s)
      Iino R, Noji H
    • Journal Title

      Current Opinion in Structural Biology

      Volume: 23 Issue: 2 Pages: 229-234

    • DOI

      10.1016/j.sbi.2013.01.004

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Winding single-molecule double-stranded DNA on a nanometer-sized reel.2012

    • Author(s)
      You H, Iino R, Watanabe R, Noji H.
    • Journal Title

      Nucleic Acids Research, Published online

      Volume: 12 Issue: 19 Pages: 1-6

    • DOI

      10.1093/nar/gks651

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Label-free single-particle imaging of the influenza virus by objective-type total internal reflection dark-field microscopy2012

    • Author(s)
      Enoki S
    • Journal Title

      PLoS One

      Volume: 7(11) Issue: 11 Pages: e49208-e49208

    • DOI

      10.1371/journal.pone.0049208

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Dynamics of linear and rotary molecular motors revealed by gold nanoprobes2015

    • Author(s)
      Ryota Iino
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Honolulu, Hawaii, USA (Hawaii Convention Center)
    • Year and Date
      2015-12-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Single-molecule imaging analysis of molecular motors2015

    • Author(s)
      Ryota Iino
    • Organizer
      PACCON2015
    • Place of Presentation
      Bangkok, Thailand (Amari Watergate Bangkok)
    • Year and Date
      2015-01-22
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Importance of membrane pumps and channels: an introduction2014

    • Author(s)
      飯野亮太
    • Organizer
      第52回生物物理学会年会
    • Place of Presentation
      北海道札幌市(札幌コンベンションセンター)
    • Year and Date
      2014-09-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Motions of individual biomolecular motors probed by gold nanoparticle and nanorod2014

    • Author(s)
      Ryota Iino
    • Organizer
      International Symposium on Small Particles and Inorganic Clusters (ISSPIC XVII)
    • Place of Presentation
      Fukuoka, Fukuoka, Japan (Kyushu University)
    • Year and Date
      2014-09-08
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Watching and manipulating biomolecules one at a time2014

    • Author(s)
      Ryota Iino
    • Organizer
      PITTCON 2014
    • Place of Presentation
      米国、McCormick Place in Chicago, Illinois
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 天然ナノモーターと人工ナノローターの1分子ダイナミクス2014

    • Author(s)
      飯野亮太
    • Organizer
      第三回 次世代の物質科学・ナノサイエンスを探る
    • Place of Presentation
      北海道、北海道大学百年記念会館
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] タンパク質分子機械の1分子計測のこれから : 改造して理解するデザイン原理2013

    • Author(s)
      飯野亮太
    • Organizer
      自然科学研究機構 分子科学研究所 所長招へいセミナー
    • Place of Presentation
      自然科学研究機構分子科学研究所(愛知県)(招待講演)
    • Year and Date
      2013-03-05
    • Related Report
      2012 Annual Research Report
  • [Presentation] 1分子蛍光観測による次世代リニアモータータンパク質セルラーゼのセルロースへの吸脱着ダイナミクスの解明2013

    • Author(s)
      柴藤祐介、中村彰彦、内橋貴之、五十嵐圭日子、野地博行、飯野亮太
    • Organizer
      第2回生物物理関東支部研究会
    • Place of Presentation
      東京農工大学(東京都)
    • Year and Date
      2013-03-04
    • Related Report
      2012 Annual Research Report
  • [Presentation] Single-molecule imaging analysis of cellobiohydrolase hydrolyzing crystalline cellulose2013

    • Author(s)
      Shibafuji Y, Nakamura A, Sugimoto N, Igarashi K, Fukuda S, Watanabe H, Uchihashi T, Noji H, Iino R
    • Organizer
      日本生物物理学会第51回年会
    • Place of Presentation
      京都府、国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] Molecular mechanism of biological nanomachines probed by single-molecule techniques2013

    • Author(s)
      Ryota Iino
    • Organizer
      12th International Conference on Atomically Controlled Surfaces, Interfaces and Nanostructures (ACSIN-12)
    • Place of Presentation
      茨城県、Tsukuba International Congress Center, Tsukuba
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] You can observe a lot just by watching motions of individual molecules2013

    • Author(s)
      Ryota Iino
    • Organizer
      Japanese-German Frontiers of Science Symposium
    • Place of Presentation
      京都府、KYOTO BRIGHTON HOTEL
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 金プローブで観た生体分子モーターの動き2013

    • Author(s)
      飯野亮太
    • Organizer
      新世代研究所 2013年度第1回バイオ単分子研究会
    • Place of Presentation
      岩手県、ホテル紫苑
    • Related Report
      2013 Annual Research Report
  • [Presentation] 倒立型顕微鏡を用いたリアルタイム1分子イメージング:蛍光法と暗視野法2013

    • Author(s)
      飯野亮太
    • Organizer
      第51回生物物理学会年会 オリンパスランチョンセミナー
    • Place of Presentation
      京都府、国立京都国際会館
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 生体分子モーターの揺らぎと機能2013

    • Author(s)
      飯野亮太
    • Organizer
      国際高等研究所 「分子基盤に基づく生体機能への揺らぎとダイナミックネットワークの解明」 第2回研究会
    • Place of Presentation
      京都府、国際高等研究所
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ATP駆動モーターの揺らぎとメカニズム2012

    • Author(s)
      飯野亮太、椶佐和子、野地博行
    • Organizer
      新学術領域「揺らぎと生体機能」「水和とATP」合同公開シンポジウム「ゆらぎと水一生命のエネルギーと機能の分子機構を探る」
    • Place of Presentation
      大阪ガーデンパレス(大阪府)(招待講演)
    • Year and Date
      2012-09-14
    • Related Report
      2012 Annual Research Report
  • [Book] DOJIN BIOSCIENCEシリーズ「揺らぎ・ダイナミクスと生体機能-物理化学的視点からみた生体分子-」Part III 機能とダイナミクス 第15章1節 モータータンパク質2013

    • Author(s)
      飯野亮太
    • Total Pages
      10
    • Publisher
      化学同人
    • Related Report
      2013 Annual Research Report
  • [Book] 先端バイオマテリアルハンドブック 秋吉一成、石原一彦、山岡哲二 監修.第4編第2章14節「全反射照明蛍光顕微鏡」2012

    • Author(s)
      飯野亮太
    • Total Pages
      6
    • Publisher
      NTS
    • Related Report
      2012 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi