Head-head coordination mechanism of kinesin during processive motility
Project/Area Number |
24370063
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Biophysics
|
Research Institution | The University of Tokyo |
Principal Investigator |
TOMISHIGE Michio 東京大学, 工学(系)研究科(研究院), 准教授 (50361530)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2012: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
|
Keywords | 分子モーター / 細胞内輸送 / 一分子計測(SMD) / ナノマシン / 構造変化 / 生物物理 / 一分子計測(SMD) |
Outline of Final Research Achievements |
Molecular motor kinesin walks along microtubules by alternately moving two motor heads, although the underlying mechanism for the head-head coordination is still unknown. Here we employed single molecule measurements with high temporal and spatial resolutions based on dark-field microscopy, and could observe intermediate states during the stepping motion of the head and the allosteric conformational change of the head. Based on these observations we showed that the direction and tension of the neck linker play pivotal roles on the head-head coordination.
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Report
(4 results)
Research Products
(23 results)