Higher order structure and function of retinal proteins
| Project/Area Number |
24370066
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Nagoya University |
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Principal Investigator |
KOUYAMA tsutomu 名古屋大学, 理学(系)研究科(研究院), 教授 (30170210)
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| Co-Investigator(Kenkyū-buntansha) |
MURAKAMI Midori 名古屋大学, 理学研究科, 助教 (20324387)
IHARA Kunio 名古屋大学, 遺伝子実験施設, 准教授 (90223297)
IWASA Tatsuo 室蘭工業大学, 工学部, 教授 (00133926)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Keywords | レチナールタンパク質 / プロトン輸送性ロドプシン / 陰イオン輸送性ロドプシン / 無脊椎動物系ロドプシン / X線結晶構造解析 / ハロロドプシン / アーキロドプシン / 反応中間体 / プロトンポンプ / 塩素イオンポンプ / 光駆動イオンポンプ / 視物質ロドプシン / 古細菌型ロドプシン / レチナール蛋白質 / 光駆動プロトンポンプ / 視物質 / 光遺伝学 / G蛋白質共役レセプター / 光センサー |
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| Outline of Final Research Achievements |
① Archaerhodopsin-2 was crystallized and its structure was determined at 1.8 Åresolution. ② Cruxrhodopsin-3 was crystallized and its structure was determined at 2.1 Å resolution. ③ Deltarhodopsin-3 was crystallized and its trimeric structure was determined at 2.7 Å resolution. ④ The light-driven chloride ion pump halorhodopsin (pHR) from Natronomonas pharonis was crystallized, and the major photoreaction states (L1, L2, N, N’and O states) of pHR-bromide ion complex were determined at 2.2 -2.7 Å resolutions. ⑤ The crystal structure of an M intermediate of pHR-azide complex was determined at 2.3 Å resolution. ⑥ Various mutants of phoborhodopsin from Halobacterium salinarum were prepared and their photoreaction kinetics were investigated. ⑦ The crystal structure of the Lumi state of squid rhodopsin was determined at 2.7 Å resolution. ⑧ Octopus rhodopsin was crystallized by the membrane fusion method.
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Report
(4 results)
Research Products
(67 results)
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[Journal Article] Searching for genomic region of high-fat diet-induced type 2 diabetes in mouse chromosome 2 by analysis of congenic strains2014
Author(s)
4.Kobayashi M, Ohno T, Ihara K, Murai, A., Kumazawa, M., Hoshino, H., Iwanaga, K., Iwai, H., Hamana, Y., Ito, M., Ohno, K., Horio, M..
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Enantioselective diels-alder reaction of 1,2-dihydropyridines with aldehydes using beta-amino alcohol organocatalyst2014
Author(s)
Kohari Y, Okuyama Y, Kwon E, Furuyama T, Kobayashi N, Otuki T, Kumagai J, Seki C, Uwai K, Dai G, Iwasa T, Nakano H
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Journal Title
The Journal of Organic Chemistry
Volume: 79
Pages: 9500-9511
DOI
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Peer Reviewed / Open Access
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[Journal Article] A blue-shifted light-driven proton pump for neural silencing2013
Author(s)
Sudo, Y., Okazaki, A., Ono, H., Yagasaki, J., Sugo, S., Kamiya, M., Reissig, L., Inoue, K., Ihara, K., Kandori, H., Takagi, S., Hayashi, S.
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Journal Title
J Biol Chem
Volume: 288
Pages: 20624-20624
DOI
Related Report
Peer Reviewed
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[Journal Article] Exome sequencing of senescence-accelerated mice (SAM) reveals deleterious mutations in degenerative disease-causing genes.2013
Author(s)
Tanisawa K., Mikami E., Fuku N., Honda Y., Honda S., Ohsawa I., Ito M., Endo S., Ihara K., Ohno K., Kishimoto Y., Ishigami A., Maruyama N., Sawabe M., Iseki H., Okazaki Y., Hasegawa-Ishii S., Takei S., Shimada A., Hosokawa M., Mori M., Higuchi K., Takeda T., Higuchi M., Tanaka M.
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Journal Title
Exome sequencing of senescence-accelerated mice (SAM) reveals deleterious mutations in degenerative disease-causing genes
Volume: 14(1):
Pages: 1-15
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] クラックスロドプシンの3量体構造と光安定性2014
Author(s)
S. K. Chan, T. Kitajima, M. Murakami, K. Ihara, T. Kouyama
Organizer
日本生物物理学会第52回年会
Place of Presentation
札幌
Year and Date
2014-09-25 – 2014-09-27
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