Tension-induced conformational changes of actin filaments and their influence on actin binding and intracellular localization of myosin.

• Project/Area Number: 24370069
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biophysics
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: UYEDA Taro 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 総括研究主幹 (90356551)
• Co-Investigator(Kenkyū-buntansha): YUMURA Shigehiko 山口大学, 医学研究科, 教授 (70183986) ; HAGA Hisashi 北海道大学, 先端生命科学研究科, 教授 (00292045) ; NOGUCHI Taro 都城工業高等専門学校, 物質工学科, 講師 (90615866)
• Research Collaborator: ADACHI Taiji 京都大学, 再生医科学研究所, 教授 (40243323)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2013: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000); Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: アクチン / ミオシン / メカノセンシティビティ / メカノセンサー / 構造多型性 / 細胞運動 / 細胞極性 / MDシミュレーション / 蛍光プローブ

Outline of Final Research Achievements

To elucidate the mechanism by which actin filaments perform different functions in vivo, we tested the hypothesis that tension applied to actin filaments increases affinity for myosin II. We were able to estimate the tension applied to actin filaments in amoeba cells. Experimental system to visualize myosin binding to tensed actin filaments in vitro was established, opening an avenue to directly test the hypothesis in vitro in the future. Some progress was also made in attempts to visualize tensed actin filaments in amoeba cells using a FRET-based system, demonstrating that the actin filaments in cells are polymorphic.

Research Products

• [Journal Article] Cofilin-induced unidirectional cooperative conformational changes in actin filaments revealed by high-speed atomic force microscopy (2015)
  • Author(s): Ngo, K.X., Kodera, N., Katayama, E., Ando, T., Uyeda, T.Q.P.
  • Journal Title: eLife Volume: 4
  • DOI: 10.7554/elife.04806
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Role of Structural Dynamics of Actin in Class-Specific Myosin Motility (2015)
  • Author(s): Noguchi, T.Q.P., Morimatsu, M., Iwane, A.H., Yanagida, T., Uyeda, T.Q.P.
  • Journal Title: PLoS One Volume: 10 Issue: 5 Pages: e0126262-e0126262
  • DOI: 10.1371/journal.pone.0126262
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Compressive stress induces dephosphorylation of the myosin regulatory light chain via RhoA phosphorylation by the adenylyl cyclase/protein kinase A signaling pathway. (2015)
  • Author(s): Takemoto, T., Ishihara, S., Mizutani, T., Kawabata, K., Haga, H.
  • Journal Title: PLoS One Volume: 10 Issue: 3 Pages: e0117937-e0117937
  • DOI: 10.1371/journal.pone.0117937
  • NAID: 120005613567
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of kinases and regulatory proteins required for cell migration using a transfected cell-microarray system (2015)
  • Author(s): Onuki-Nagasaki R, Nagasaki A, Hakamada K, Uyeda T, Miyake M, Miyake J, Fujita S
  • Journal Title: BMC Genetics Volume: 16 Issue: 1 Pages: 9-20
  • DOI: 10.1186/s12863-015-0170-7
  • Peer Reviewed / Open Access
• [Journal Article] Myosin II does not contribute to wound repair in Dictyostelium cells. (2014)
  • Author(s): Yumura, S., Hashima, S., Muranaka, S.
  • Journal Title: Biol. Open Volume: 3 Issue: 10 Pages: 966-973
  • DOI: 10.1242/bio.20149712
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Modulation of extracellular conditions prevents the multilayering of the simple epithelium (2014)
  • Author(s): T. Mizutani, K. Takeda, H. Haga, M. Todo, K. Kawabata
  • Journal Title: Histochemistry and Cell Biology Volume: Vol. 141(5) Issue: 5 Pages: 459-471
  • DOI: 10.1007/s00418-013-1176-8
  • Peer Reviewed
• [Journal Article] Coating extracelular matrix proteins on a (3-aminopropyl)triethoxysilane-treated glass substrate for improved cell culture. (2014)
  • Author(s): Masuda, H.T., Ishihara, S., Harada, I., Mizutani, T., Ishikawa, M., Kawabata, K., Haga, H..
  • Journal Title: Biotechniques Volume: 56 Issue: 4 Pages: 172-179
  • DOI: 10.2144/000114156
  • Peer Reviewed
• [Journal Article] Structural and Functional Differentiation of Actin Filaments (2014)
  • Author(s): 上田太郎
  • Journal Title: Seibutsu Butsuri Volume: 54 Issue: 2 Pages: 100-103
  • DOI: 10.2142/biophys.54.100
  • NAID: 130003391415
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed
• [Journal Article] Contraction velocity of the actomyosin cytoskeleton in the absence of cell membrane. (2013)
  • Author(s): Plaza, G. R., T. Q. P. Uyeda
  • Journal Title: Soft Matter Volume: 9 Issue: 17 Pages: 4390-4400
  • DOI: 10.1039/c3sm27867k
  • Peer Reviewed
• [Journal Article] ADF/cofilin is not essential but is crucially important for actin activities during phagocytosis in Tetrahymena thermophila. (2013)
  • Author(s): Shiozaki, N., Nakano, K., Kushida, Y., Noguchi, T.Q.P., Uyeda, T.Q.P., Wloga, D., Dave, D., Vasudevan, K. K., Gaertig, J., Numata, O.
  • Journal Title: Eukaryotic Cell Volume: 12 Issue: 8 Pages: 1080-1086
  • DOI: 10.1128/ec.00074-13
  • Peer Reviewed
• [Journal Article] Bulkiness or aromatic nature of tyrosine-143 of actin is important for the weak binding between F-actin and myosin-ADP-phosphate. (2013)
  • Author(s): Gomibuchi, Y., Uyeda, T. Q. P., Wakabayashi, T.
  • Journal Title: Biochem. Biophys. Res Commun. Volume: 441 Issue: 4 Pages: 844-848
  • DOI: 10.1016/j.bbrc.2013.10.144
  • Peer Reviewed
• [Journal Article] Substrate stiffness regulates temporary NF-κB activation via actomyosin contractions (2013)
  • Author(s): Seiichiro Ishihara, Motoaki Yasuda, Ichiro Harada, Takeomi Mizutani, Kazushige Kawabata, Hisashi Haga
  • Journal Title: Experimental Cell Research Volume: 319(19) Issue: 19 Pages: 2916-2927
  • DOI: 10.1016/j.yexcr.2013.09.018
  • Peer Reviewed
• [Journal Article] Dynamin contributes to cytokinesis by stabilizing actin filaments in the contractile ring (2013)
  • Author(s): Masud Rana, A.Y. K. Md., Tsujioka, M., Miyagishima, S., Ueda, M. and Yumura, S.
  • Journal Title: Genes to Cells Volume: 18 Issue: 8 Pages: 621-635
  • DOI: 10.1111/gtc.12060
  • Peer Reviewed
• [Journal Article] Cell-scale dynamic recycling arid cortical flow of the actin-myosin cytoskeleton for rapid cell migration (2013)
  • Author(s): Yumura, S., Itoh, G., Kikuta, Y., Kikuchi, T., Kitanishi-Yumura, T. and Tsujioka, M.
  • Journal Title: Biol. Open Volume: 2 Issue: 2 Pages: 200-209
  • DOI: 10.1242/bio.20122899
  • Peer Reviewed
• [Journal Article] Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain (2013)
  • Author(s): Ishihara S, Yasuda M, Nishioka T, Mizutani T, Kawabata K, Shirato H, Haga H
  • Journal Title: FEBS Lett Volume: 587(6) Issue: 6 Pages: 732-736
  • DOI: 10.1016/j.febslet.2013.01.055
  • NAID: 120005228631
  • Peer Reviewed
• [Journal Article] G146V mutation at the hinge region of actin reveals a myosin class-specific requirement of actin conformations for motility. (2012)
  • Author(s): Noguchi, T. Q. P., Komori, T., Umeki, N., Demizu, N., Ito, K., Iwane, A. H., Tokuraku, K., Yanagida, T., Uyeda, T. Q. P.
  • Journal Title: J. Biol. Chem. Volume: 279 Issue: 29 Pages: 24399-34345
  • DOI: 10.1074/jbc.m111.321752
  • Peer Reviewed
• [Journal Article] Rapid nucleotide exchange renders Asp-11 mutant actins resistant to depolymerizing activity of cofilin, leading to dominant toxicity in vivo. (2012)
  • Author(s): Umeki, N., Nakajima, J., Noguchi, T.Q.P., Tokuraku, K., Nagasaki, A., Ito, K., Hirose, K. and Uyeda, T.Q.P.
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 3 Pages: 1739-1748
  • DOI: 10.1074/jbc.m112.404657
  • Peer Reviewed
• [Journal Article] Talin couples the actomyosin cortex to the plasma membrane during rear retraction and cytokinesis. (2012)
  • Author(s): Tsujioka, M., Yumura, S., Inouye, K., Patel, H., Ueda, M. and Yonemura, S.
  • Journal Title: Proc. Natl. Acad. Sci. USA. Volume: 109 Issue: 32 Pages: 12992-12997
  • DOI: 10.1073/pnas.1208296109
  • Peer Reviewed
• [Journal Article] Neuronal correlates of multiple top-down signals during covert tracking of moving objects in macaque prefrontal cortex (2012)
  • Author(s): Matsushima, A. & Tanaka, M.
  • Journal Title: J. Cogn. Neurosci Volume: 24 Issue: 01 Pages: 2043-2056
  • DOI: 10.4236/cellbio.2012.11001
  • NAID: 120005090600
  • Peer Reviewed
• [Presentation] Functional differentiation of actin filaments mediated by cooperative conformational changes of the filament structure (2015)
  • Author(s): Uyeda, T.Q.P., Kodera, N., and Tokuraku, K.
  • Organizer: 日本生物物理学会第53回年会
  • Place of Presentation: 金沢大学、金沢
  • Year and Date: 2015-09-13
  • Invited
• [Presentation] 協同的構造変化を介したアクチンフィラメントの機能制御 (2015)
  • Author(s): 上田太郎、古寺哲幸、徳楽清孝
  • Organizer: 第67回日本細胞生物学会大会
  • Place of Presentation: タワーホール船堀、東京
  • Year and Date: 2015-07-02
  • Invited
• [Presentation] Comparision of traction force among three modes of cytokinesis in Dictyostelium. (2014)
  • Author(s): Jahan, S., Yumura, S.
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2014-11-25
• [Presentation] アクチン結合タンパク質のアクチン結合ドメインは細胞の前後特異的に局在する (2014)
  • Author(s): 柴田桂太朗、長崎晃、辻岡政経、髙稲正勝、中野賢太郎、沼田治、上田太郎
  • Organizer: 日本細胞性粘菌学会第4回例会
  • Place of Presentation: 東北大学、仙台
  • Year and Date: 2014-10-12
• [Presentation] Cofilin-induced unidirectional cooperative conformational changes of actin filaments visualized by high speed atomic force microscopy (2014)
  • Author(s): Ngo, K.X., 古寺哲幸、片山栄作、長崎晃、安藤敏夫、上田太郎
  • Organizer: 日本生物物理学会第52回年会
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2014-09-26
• [Presentation] The motility of Myosin IXb depend on difference of actin filaments (2014)
  • Author(s): 山田正文、梅木伸久、池辺光男、上田太郎
  • Organizer: 日本生物物理学会第52回年会
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2014-09-26
• [Presentation] Arabidopsis thaliana actin isoforms do not bind phalloidin (2014)
  • Author(s): 貴嶋紗久、孔三根、森正之、和田正三、上田太郎
  • Organizer: 日本生物物理学会第52回年会
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2014-09-25
• [Presentation] Dictyostelium cells carrying the plasmids to express mutant actin (Tyr143Phe) are more susceptible to high-pressure treatment. (2014)
  • Author(s): 五味渕由貴、大貫貴広、上田太郎、西山雅祥、若林健之
  • Organizer: 日本生物物理学会第52回年会
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2014-09-25
• [Presentation] Actin binding domains of certain actin binding proteins are sufficient to localize at specific F-actin in vivo (2014)
  • Author(s): 柴田桂太朗、長崎晃、辻岡政経、上田太郎
  • Organizer: 日本生物物理学会第52回年会
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2014-09-25
• [Presentation] Mutations introduced into the hydrophobic helix of the Dictyostelium actin impaired cell motility and cytokinesis (2014)
  • Author(s): 大貫貴広、五味渕由貴、上田太郎、若林健之
  • Organizer: 日本生物物理学会第52回年会
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2014-09-25
• [Presentation] Piezoelectric property of an actin filament (2014)
  • Author(s): 大貫準、佐藤昴人、梅沢公二、上田太郎、高野光則
  • Organizer: 日本生物物理学会第52回年会
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2014-09-25
• [Presentation] FRETプローブアクチンを用いた細胞内におけるアクチンの構造多型の検出 (2014)
  • Author(s): 岡崎美緒、貴嶋紗久、岩楯好昭、上田太郎、野口太郎
  • Organizer: 日本生物物理学会第52回年会
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2014-09-25
• [Presentation] 高速AFMと蛍光顕微鏡観察によるF-アクチンへのHMM、コフィリンの協同的結合の解析 (2014)
  • Author(s): 上野寛朗、西川雄亮、相内瑛、平川利佳、吉野敦貴、古寺哲幸、上田太郎、徳楽清孝
  • Organizer: 日本生物物理学会第52回年会
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2014-09-25
• [Presentation] 微小管結合蛋白質MAP2、MAP4、TauのF-アクチン-微小管束化活性の違い (2014)
  • Author(s): 齊藤翔馬、倉本歩、槇原光、志賀美由貴、小谷享、徳楽清孝
  • Organizer: 日本生物物理学会第52回年会
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2014-09-25
• [Presentation] シロイヌナズナアクチンの諸性質：ミオシンとの相互作用を中心に (2014)
  • Author(s): 諏訪貴大、大矢里美、貴嶋紗久、富永基樹、大木高志、中野明彦、石渡信一、上田太郎、伊藤光二
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学生田キャンパス、川崎
  • Year and Date: 2014-09-13
• [Presentation] 植物アクチンアイソフォームの精製・機能解析 (2014)
  • Author(s): 貴嶋紗久、孔三根、和田正三、上田太郎
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学生田キャンパス、川崎
  • Year and Date: 2014-09-13
• [Presentation] 細胞運動における各セルイベントを制御するシグナル経路の探索 (2014)
  • Author(s): 長崎晃、上田太郎、長崎玲子、藤田聡史
  • Organizer: 第9回細胞運動研究会
  • Place of Presentation: 広島大学、東広島
  • Year and Date: 2014-09-03
• [Presentation] アクチンフィラメントの構造多型と機能分化 (2014)
  • Author(s): 上田太郎
  • Organizer: 理研シンポジウム第４回分子モーター討論会
  • Place of Presentation: 大阪大学、豊中
  • Year and Date: 2014-06-27
  • Invited
• [Presentation] 優性にミオパチーを引き起こすDH変異アクチンは、ヌクレオチド解離・交換が速いため、コフィリンと相互作用できない (2013)
  • Author(s): 梅木伸久、中嶋潤、野口太郎、徳楽清孝、長崎晃、伊藤光二、広瀬恵子、上田太郎
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 広島
  • Year and Date: 2013-01-12
• [Presentation] Role of dynamin in Dictyostelium cytokinesis. (2012)
  • Author(s): Rana,.A.Y.Md M. and Yumura S.
  • Organizer: 第85回日本生化学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-14
• [Presentation] Contraction speed of the actomyosin cortex in the absence of cell membrane. (2012)
  • Author(s): グスタボ・プラザ、上田太郎
  • Organizer: 第2回日本細胞性粘菌学会例会
  • Place of Presentation: 東京
  • Year and Date: 2012-11-17
• [Presentation] Cooperative conformational changes of actin filaments drive mutually exclusive cooperative binding of cofilin and HMM to the filaments. (2012)
  • Author(s): Umeki N, Uyeda TQ.
  • Organizer: 日本生物物理学会第50回年会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-23
• [Presentation] Structural polymorphism and functional differentiation of actin filaments (2012)
  • Author(s): 上田太郎、梅木伸久、貴嶋紗久、西川雄亮、徳楽清孝、長崎晃、野口太郎
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 名古屋(招待) )
  • Year and Date: 2012-09-23
• [Presentation] Analysis of cooperative interaction of Dictyostelium-derived cofilin and HMM to F-actin using fluorescence microscopy. (2012)
  • Author(s): 西川雄亮、佐藤あゆみ、上田太郎、徳楽清孝
  • Organizer: 日本生物物理学会第50回年会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-23
• [Presentation] ミオシン結合により誘起されるアクチンフィラメントの協同的構造変化-アクチンフィラメントの構造多型と機能分化- (2012)
  • Author(s): 上田太郎
  • Organizer: 公開シンポジウム「ゆらぎと水一生命のエネルギーと機能の分子機構を探る」
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-15
• [Presentation] Dynamin affects cytokinesis in Dictyostelium cells. (2012)
  • Author(s): Rana,.A.Y.Md M. and Yumura S
  • Organizer: 第64回日本細胞生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2012-05-28
• [Presentation] Involvement of cell polarity in folding phenomena of epithelial sheets (2012)
  • Author(s): 石田すみれ、田中良昌、水谷武臣、川端和重、芳賀永
  • Organizer: 第64回日本細胞生物学会大会
  • Place of Presentation: 神戸国際会議場(神戸市)
• [Presentation] Live cell imaging of collective migration in 3D morphogenesis of epithelial cells (2012)
  • Author(s): 今井美沙子、水谷武臣、川端和重、芳賀永
  • Organizer: 第64回日本細胞生物学会大会
  • Place of Presentation: 神戸国際会議場(神戸市)
• [Presentation] Invasive migration of irradiation-survived human lung adenocarcinoma cells in 3D matrix (2012)
  • Author(s): 芳賀永
  • Organizer: 第31回札幌国際がんシンポジウム
  • Place of Presentation: 北海道大学(札幌市)(招待講演)
• [Presentation] Collective behavior of epithelial cells responding to stiffness, roughness and Composition of extracellular matrix (2012)
  • Author(s): 芳賀永
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場(福岡市)(招待講演)
• [Presentation] Involvement of cell polarity and cell migration in folding phenomena of epith elial sheets (2012)
  • Author(s): S. Ishida, R. Tanaka, N. Yamaguchi, T. Mizutani, K. Kawabata, H. Haga
  • Organizer: 第52回米国細胞生物学会年会
  • Place of Presentation: モスコーンセンター(米国,サンフランシスコ)
• [Presentation] 細胞運動に関与する遺伝子として同定したホスホリパーゼDとキナーゼ群の細胞生物学的解析およびパスウェイ解析
  • Author(s): 長崎玲子、上田太郎、藤田聡史、長崎晃
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: ウインクあいち（名古屋市）
• [Presentation] アクチン重合、ミオシンATP 加水分解活性化に対するTyr143 変異の効果
  • Author(s): 五味渕由貴、上田太郎、若林健之
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都国際会議場（京都市）
• [Presentation] ガラス基板上に固定したF-アクチンへのコフィリンとHMMと協同的結合
  • Author(s): 西川雄亮、上野寛朗、相内瑛、上田太郎、徳楽清孝
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都国際会議場（京都市）
• [Presentation] Actin mutation introduced into the hydrophobic helix impairs cytokinesis of Dictyostelium cell
  • Author(s): 大貫貴広、五味渕由貴、上田太郎、若林健之
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都国際会議場（京都市）
• [Presentation] Myosin mutant G680V accelerated sliding velocities of skeletal muscle acto-myosin
  • Author(s): 岩瀬航平、田中正輝、上田太郎、本多元
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都国際会議場（京都市）
• [Presentation] Piezoelectric effect in a protein and its involvement in allosteric regulation
  • Author(s): 大貫準、佐藤昴人、梅沢公二、上田太郎、高野光則
  • Organizer: 日本生物物理学会第51回年会
  • Place of Presentation: 京都国際会議場（京都市）
• [Presentation] Piezoelectricity of protein and its implication for function.
  • Author(s): 大貫準、佐藤昴人、梅沢公二、上田太郎、高野光則
  • Organizer: 国際分子シミュレーション討論会
  • Place of Presentation: 神戸コンベンションセンター（神戸市）
• [Presentation] 種々のGFP-アクチン結合ドメインの細胞内局在と、細胞の前後極性の関係
  • Author(s): 柴田桂太朗、長崎晃、辻岡政経、上田太郎
  • Organizer: 日本細胞性粘菌学会第3回例会
  • Place of Presentation: 京都大学（京都市）
• [Presentation] Video Imaging of Cofilin-Induced Actin Filament Severing by High Speed AFM
  • Author(s): Ngo Xuan Kien, 古寺哲幸、長崎晃、安藤敏夫、上田太郎
  • Organizer: Biophysical Society 57th Annual Meeting
  • Place of Presentation: Moscone Convention Center, San Francisco, USA
• [Presentation] 細胞性粘菌で同定した細胞運動制御遺伝子ホスホリパーゼDの1分子解析
  • Author(s): 長崎晃、猪爪貴美子、上田太郎
  • Organizer: 日本細胞性粘菌学会第3回例会
  • Place of Presentation: 京都大学（京都市）
• [Book] アクチン・ミオシン系細胞運動:分裂の分子機械 ln細胞性粘菌:研究の新展開:モデル生物・創薬資源・バイオ (2012)
  • Author(s): 祐村恵彦、上田太郎
  • Total Pages: 24
  • Publisher: アイピーシー社
• [Remarks] セルメカニクス研究グループ
  • URL: https://staff.aist.go.jp/t-uyeda/HP/