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Development of a 3D high-resolution component analysis system and observation of biological specimens

Research Project

Project/Area Number 24370070
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Biophysics
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

OGURA Toshihiko  独立行政法人産業技術総合研究所, バイオメディカル研究部門, 上級主任研究員 (70371028)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Keywords電子顕微鏡 / 非染色生物試料 / 液中観察 / 薄膜 / 誘電率 / 3次元構造解析 / 画像情報処理 / 生命分子計算 / 3次元構造解析
Outline of Final Research Achievements

To understand various biological functions, observation of the nanometre structures of bacteria and viruses is essential. Scanning electron microscopy (SEM) is an important tool for such observations. However, samples of SEM are usually prepared by staining to enhance image contrast. Staining preparation is often not recommended to observe detail of the biological structures. Moreover, biological samples are prone to radiation damage and unstained samples give very poor contrast. Here, we report new methods for the unstained biological specimens in water of bacteria and virus, proteins. The obtained images show very high-contrast, and the samples incur low-electron radiation damage. Our method can be used for diverse liquid specimens across a broad range, such as nanoparticles and catalytic materials.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (13 results)

All 2015 2014 2012

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 2 results,  Acknowledgement Compliant: 3 results) Presentation (6 results)

  • [Journal Article] Nanoscale analysis of unstained biological specimens in water without radiation damage using high-resolution frequency transmission electric-field system based on FE-SEM2015

    • Author(s)
      Toshihiko Ogura
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 459 Issue: 3 Pages: 521-528

    • DOI

      10.1016/j.bbrc.2015.02.140

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Non-destructive observation of intact bacteria and viruses in water by the highly sensitive frequency transmission electric-field method based on SEM2014

    • Author(s)
      Toshihiko Ogura
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 450 Issue: 4 Pages: 1684-1689

    • DOI

      10.1016/j.bbrc.2014.07.062

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] New simulated annealing approach considering helix bending applied to determine the 8.8 A structure of 15-protofilament microtubules2014

    • Author(s)
      T.Ogura, H. Yajima, R.Nitta, N.Hirokawa, C.Sato
    • Journal Title

      J Struct Biol

      Volume: 188 Issue: 2 Pages: 165-176

    • DOI

      10.1016/j.jsb.2014.08.009

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Direct scanning electron microscopy-based observation of dispersed core shell-type nanoparticles in a wet state2014

    • Author(s)
      Noriya Izua, Toshihiko Ogura, Takafumi Akamatsu, Toshio Itoh, Woosuck Shin
    • Journal Title

      Ceramics International

      Volume: 40 Pages: 16361-16364

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct observation of unstained biological specimens in water by the frequency transmission electric-field method using SEM2014

    • Author(s)
      Toshihiko Ogura
    • Journal Title

      PLoS ONE

      Volume: 9 Issue: 3 Pages: e92780-e92780

    • DOI

      10.1371/journal.pone.0092780

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct observation of the inner structure of unstained atmospheric cells by low-energy electrons2012

    • Author(s)
      Toshihiko Ogura
    • Journal Title

      Measurement Science & Technology

      Volume: Vol.23 Issue: 8 Pages: 085402-085402

    • DOI

      10.1088/0957-0233/23/8/085402

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-contrast observation of unstained proteins and viruses by scanning electron microscopy2012

    • Author(s)
      Toshihiko Ogura
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 10 Pages: e46904-e46904

    • DOI

      10.1371/journal.pone.0046904

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 変動電位透過観察法による水溶液中の非染色・非固定ウイルスの直接観察2014

    • Author(s)
      小椋 俊彦
    • Organizer
      日本ウイルス学会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 走査電子顕微鏡による大気圧下の非染色バクテリアの高コントラスト観察法2012

    • Author(s)
      小椋俊彦
    • Organizer
      極限環境生物学会
    • Place of Presentation
      東京 日本大学
    • Year and Date
      2012-12-02
    • Related Report
      2012 Annual Research Report
  • [Presentation] 走査電顕による非染色ウイルス試料の高コントラスト・低ダメージ観察方法2012

    • Author(s)
      小椋俊彦
    • Organizer
      日本ウイルス学会
    • Place of Presentation
      大阪
    • Year and Date
      2012-11-14
    • Related Report
      2012 Annual Research Report
  • [Presentation] 走査電子顕微鏡による非染色バクテリアサンプルの高コントラスト・低ダメージ観察法2012

    • Author(s)
      小椋俊彦
    • Organizer
      日本食品微生物学会
    • Place of Presentation
      福岡市
    • Year and Date
      2012-10-25
    • Related Report
      2012 Annual Research Report
  • [Presentation] A high-contrast and low-damage observation method of the unstained biological samples by scanning-electron microscop2012

    • Author(s)
      小椋俊彦
    • Organizer
      日本生物物理学会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2012-09-24
    • Related Report
      2012 Annual Research Report
  • [Presentation] 走査電子線を用いた非染色生物試料の高コントラスト・低ダメージ観察方法2012

    • Author(s)
      小椋俊彦
    • Organizer
      日本顕微鏡学会
    • Place of Presentation
      つくば(招待公演)
    • Year and Date
      2012-05-16
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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