|Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
|Outline of Final Research Achievements
In order to understand the detailed mechanism of the initiation of DNA replication in eukaryotic cells, we have developed a novel strategy to isolate mutants in budding yeast and have isolated tight mutants of replication factors. By using these mutants, we have analysed the mode of association of replication factors to the origin of DNA replication on chromosomal DNA and have revealed that the molecular identity and the mode of assembly of ‘pre-initiation complex (pre-IC)’, which was proposed to exist temporally just before the initiation of DNA replication and was never proved that there. The assembly of pre-IC was mutually dependent on factors that constitute the pre-IC. Such mode of regulation might contribute to the biological robustness of the initiation reaction, and finally contribute to the precise regulation of cellular proliferation.