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Establishment of the system for identification of causal viruses in fruit tree virus diseases based on virome analyses

Research Project

Project/Area Number 24380027
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Plant pathology
Research InstitutionIwate University

Principal Investigator

Yoshikawa Nobuyuki  岩手大学, 農学部, 教授 (40191556)

Co-Investigator(Kenkyū-buntansha) Ito Tsutae  国立研究開発法人農業・食品産業技術総合研究機構, 果樹茶業研究部門, 上席研究員 (20355415)
Yaegashi Hajime  国立研究開発法人農業・食品産業技術総合研究機構, 果樹茶業研究部門, 主任研究員 (90582594)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Keywords植物ウイルス学 / 植物病理学 / 果樹ウイルス病 / 病原ウイルスの同定 / バイローム解析 / 次世代シークエンス / オウトウ芽枯病 / リンゴ輪状さび果病 / リンゴモザイク病 / オウトウBウイルス / リンゴえそモザイクウイルス / オウトウ芽枯れ病 / リンゴ奇形果病 / セイヨウナシ粗皮病 / コンティグ / Foveavirus
Outline of Final Research Achievements

We conducted virome analyses by the next-generation sequencer of cherry trees affected by a bud blight disease, apple trees by a russet ring disease, apple trees by a green crinkle disease, and apple trees by a mosaic-disease. The results indicated that two new viruses, Cherry virus B and Apple necrotic mosaic virus (ApMV) were detected from a cherry tree affected by a bud blight disease and mosaic-diseased apple trees, respectively. Furthermore, we found an Apple chlorotic leaf spot virus variant from a russet ring-diseased apple trees and this variant induced a russet-like symptom on fruits of inoculated apple seedlings.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (11 results)

All 2015 2014 2013

All Presentation (11 results)

  • [Presentation] Isolation of Apple chlorotic leaf spot virus variants from a russet ring-diseased apple trees by in vitro transcription system and evaluation of their pathogenesis on fruits of apple2015

    • Author(s)
      R. Kishigami, H. Yaegashi, N. Yamagishi, T. Ito, and N. Yoshikawa
    • Organizer
      23rd International Conference on Virus and Other Graft Transmissible Diseases of Fruit Crops
    • Place of Presentation
      Aiina Hall, Miroika, Japan
    • Year and Date
      2015-06-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Apple necrotic mosaic virus (ApNMV), a new virus classified into genus Ilarvirus, found in apple trees affected by a mosaic disease.2015

    • Author(s)
      H. Noda, N. Yamagishi, H. Yaegashi, M. Isogai, T. Ito, and N. Yoshikawa
    • Organizer
      23rd International Conference on Virus and Other Graft Transmissible Diseases of Fruit Crops
    • Place of Presentation
      Aiina Hall, Miroika, Japan
    • Year and Date
      2015-06-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Multiple virus infection in cherry trees affected by a bud blight disease2015

    • Author(s)
      N. Yoshikawa, E. Oyamada, H. Yaegashi, S. Goto, N. Yamagishi, M. Isogai, T. Ito,
    • Organizer
      23rd International Conference on Virus and Other Graft Transmissible Diseases of Fruit Crops
    • Place of Presentation
      Aiina Hall, Miroika, Japan
    • Year and Date
      2015-06-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Cherry virus B, a new virus classified into genus Foveavirus found in a sweet cherry tree.2015

    • Author(s)
      S. Oyamada, H. Yaegashi, S. Goto, N. Yamagishi, M. Isogai, T. Ito, and N. Yoshikawa
    • Organizer
      23rd International Conference on Virus and Other Graft Transmissible Diseases of Fruit Crops
    • Place of Presentation
      Aiina Hall, Miroika, Japan
    • Year and Date
      2015-06-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] 感染リンゴ樹試料からRT-PCRとIn Vitro転写で合成したリンゴウイルスRNAのリンゴ実生への感染2014

    • Author(s)
      岸上隆介・山岸紀子・八重樫 元・伊藤 伝・吉川信幸
    • Organizer
      平成26年度日本植物病理学会東北部会
    • Place of Presentation
      盛岡市アイーナホール
    • Year and Date
      2014-09-25 – 2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] モザイク症状を示すリンゴから分離された新規イラルウイルス[リンゴえそモザイクウイルス, Apple necrotic mosaic virus (ANMV)](仮称)について2014

    • Author(s)
      野田浩気・山岸紀子・八重樫元・磯貝雅道・伊藤 伝・吉川信幸
    • Organizer
      平成26年度日本植物病理学会東北部会
    • Place of Presentation
      盛岡市アイーナホール
    • Year and Date
      2014-09-25 – 2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] ウイルスRNAのIn Vitro転写系を利用したリンゴ奇形果病と輪状さび果病樹からのウイルスの単離2014

    • Author(s)
      岸上隆介・山岸紀子・八重樫 元・伊藤 伝・吉川信幸
    • Organizer
      平成26年度日本植物病理学会大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-06-02 – 2014-06-04
    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
  • [Presentation] バイローム解析で明らかになった芽枯病罹病オウトウ樹の多重ウイルス感染2014

    • Author(s)
      吉川信幸・小山田早希・八重樫元・山岸紀子・後藤新一・磯貝雅道・伊藤 伝
    • Organizer
      平成26年度日本植物病理学会大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-06-02 – 2014-06-04
    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
  • [Presentation] リンゴ奇形果病罹病樹の次世代シークエンスによるバイローム解析2013

    • Author(s)
      吉川信幸・斉藤邦亮・山岸紀子・八重樫元・伊藤 伝
    • Organizer
      平成25年度日本植物病理学会大会
    • Place of Presentation
      岐阜大学(岐阜県)
    • Year and Date
      2013-03-29
    • Related Report
      2012 Annual Research Report
  • [Presentation] オウトウ芽枯病罹病樹の次世代シークエンスによるバイローム解析2013

    • Author(s)
      斉藤邦亮・山岸紀子・後藤新一・八重樫元・伊藤 伝・磯貝雅道・吉川信幸
    • Organizer
      平成25年度日本植物病理学会大会
    • Place of Presentation
      岐阜大学(岐阜県)
    • Year and Date
      2013-03-29
    • Related Report
      2012 Annual Research Report
  • [Presentation] 芽枯病罹病オウトウ樹に見いだされた新規Foveavirus属ウイルス[チェリーBウイルス(仮称)]について2013

    • Author(s)
      小山田早希・斉藤邦亮・山岸紀子・後藤新一・八重樫元・伊藤 伝・磯貝雅道・吉川信幸
    • Organizer
      平成25年度日本植物病理学会東北部会
    • Place of Presentation
      秋田市にぎわい交流館
    • Related Report
      2013 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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