Functional analysis of odorant and gustatory receptors in insects
Project/Area Number |
24380030
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Applied entomology
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Research Institution | The University of Tokyo |
Principal Investigator |
MATSUO Takashi 東京大学, 農学生命科学研究科, 准教授 (70301223)
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Co-Investigator(Kenkyū-buntansha) |
OZAKI Katsuhisa 株式会社生命誌研究館, 研究員 (60396223)
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Co-Investigator(Renkei-kenkyūsha) |
ISHIKAWA Yukio 東京大学, 農学生命科学研究科, 教授 (60125987)
TAMURA Koichiro 首都大学東京, 理工学研究科, 教授 (00254144)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
|
Keywords | 化学感覚 / de novo トランスクリプトーム解析 / asdf / フェロモン / 食草 |
Outline of Final Research Achievements |
Perception of odors and tastes plays an important role in insect ecology. Understanding chemosensory systems provides us the opportunity to understand the insect behavior and develop novel methods to control pest insects. In this study, using high-throughput DNA sequencing system, we established the method for genome wide identification of odorant- and gustatory- receptors, as well as odorant-binding proteins, in various insect species. Furthermore, we generated knock-out strains for odorant receptor genes in a non-model insect species using artificial nuclease. Combining these two methods, now it is possible to identify chemosensory genes in any insect species for functional analysis using reverse genetics.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Gustatory sensing mechanism coding for multiple oviposition stimulants in the swallowtail butterfly, Papilio xuthus.2013
Author(s)
Ryuda, M, Calas-List, D., Yamada, A, Marion-Poll, F.. Yoshikawa, R, Tanimura, T., Ozaki, K.
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Journal Title
Journal of Neuroscience
Volume: 33
Pages: 914-924
Related Report
Peer Reviewed
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