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A study on ecological functions of insect virus genes in biological interactions

Research Project

Project/Area Number 24380035
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Applied entomology
Research InstitutionForestry and Forest Products Research Institute

Principal Investigator

TAKATSUKA JUN  独立行政法人森林総合研究所, 森林昆虫研究領域, 主任研究員 (80399378)

Co-Investigator(Kenkyū-buntansha) NAKAI Madoka  東京農工大学, (連合)農学研究科(研究院), 准教授 (60302907)
KUNIMI Yasuhisa  東京農工大学, (連合)農学研究科(研究院), 教授 (50195476)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Keywords昆虫ウイルス / アワヨトウ / 生物間相互作用 / 昆虫ポックスウイルス / 適応度 / 昆虫 / ウイルス
Outline of Final Research Achievements

To analyze biological functions of the gene that is thought to be responsible for manipulation of host development, we constructed recombinant viruses, namely knock-in virus created by inserting the green fluorescent protein reporter under the control of spheroidin gene promoter (GFP cassette) into a non-coding region of virus genome, knock-out virus having the gene disrupted by a GFP cassette and rescue virus that is restored the complete gene in knock-out virus. Phenotypic comparison of wild-type and recombinant viruses-infected larvae of Mythimna separata indicated that the gene is truly responsible for manipulation of host development and metamorphosis of the infected hosts. Furthermore, the gene altered normal behavior of the host before pupation. Non-infected larvae moved from host plants to soil and formed cell for pupation. However, insects infected with viruses having the gene did not show such behavior and stayed on host plants.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (10 results)

All 2015 2014 2013 2012 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (7 results)

  • [Journal Article] Gene Acquisition Convergence between Entomopoxviruses and Baculoviruses.2015

    • Author(s)
      Theze, J., Takatsuka, J., Nakai, M., Arif, B., Herniou, EA.
    • Journal Title

      Viruses

      Volume: 7 Issue: 4 Pages: 1960-1974

    • DOI

      10.3390/v7041960

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Replication of Mythimna separata entomopoxvirus in High FiveTM cells and the construction of a recombinant2014

    • Author(s)
      Jun Takatsuka, Madoka Nakai
    • Journal Title

      Journal of Invertebrate Pathology

      Volume: 118 Pages: 12-17

    • DOI

      10.1016/j.jip.2014.02.010

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] New Insights into the Evolution of Entomopoxvirinae from the Complete Genome Sequences of Four Entomopoxviruses Infecting Adoxophyes honmai, Choristoneura biennis, Choristoneura rosaceana, and Mythimna separata2013

    • Author(s)
      Julien Theze, Jun Takatsuka, Zhen Li, Julie Gallais, Daniel Doucet, Basil Arif, Madoka Nakai, Elisabeth A. Hernioua
    • Journal Title

      Journal of Virology

      Volume: 87 Issue: 14 Pages: 7992-8003

    • DOI

      10.1128/jvi.00453-13

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 昆虫ポックスウイルスの感染経路について2015

    • Author(s)
      高務淳
    • Organizer
      日本応用動物昆虫学会
    • Place of Presentation
      山形大学(山形県山形市)
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 昆虫ポックスウイルスの世界:ゲノム、おもしろ遺伝子、ゲノム外DNA2014

    • Author(s)
      高務淳
    • Organizer
      昆虫病理研究会シンポジウム
    • Place of Presentation
      一般財団法人人材開発センター富士研修所富士Calm (山梨県富士吉田市)
    • Year and Date
      2014-09-18 – 2014-09-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 昆虫ポックスウイルス感染がアワヨトウの発育と変態に与える影響2013

    • Author(s)
      金城寛俊
    • Organizer
      昆虫病理研究会
    • Place of Presentation
      帯広畜産大学(北海道帯広市)
    • Year and Date
      2013-09-22
    • Related Report
      2012 Annual Research Report
  • [Presentation] 昆虫ポックスウイルスの戦略パート1:宿主や宿主を取り巻く生物との相互作用2013

    • Author(s)
      高務淳
    • Organizer
      日本応用動物昆虫学会
    • Place of Presentation
      日本大学(神奈川県藤沢市)
    • Year and Date
      2013-03-29
    • Related Report
      2012 Annual Research Report
  • [Presentation] 昆虫ポックスウイルスjhamtホモログ破壊株がアワヨトウ幼虫の変態に与える影響2013

    • Author(s)
      金城寛俊
    • Organizer
      日本応用動物昆虫学会
    • Place of Presentation
      日本大学(神奈川県藤沢市)
    • Year and Date
      2013-03-28
    • Related Report
      2012 Annual Research Report
  • [Presentation] Effect of entomopoxvirus infection on growth and metamorphosis of Mythimna separate larvae2012

    • Author(s)
      金城寛俊
    • Organizer
      国際昆虫学会
    • Place of Presentation
      EXCO(デグ、韓国)
    • Year and Date
      2012-08-20
    • Related Report
      2012 Annual Research Report
  • [Presentation] アワヨトウ昆虫ポックスウイルスが持つシロモンヤガ顆粒病ウイルス遺伝子のホモログについて

    • Author(s)
      高務淳、仲井まどか
    • Organizer
      日本応用動物昆虫学会
    • Place of Presentation
      高知大学
    • Related Report
      2013 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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