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Roles of autophagy in the life cycle of rice

Research Project

Project/Area Number 24380037
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionTohoku University

Principal Investigator

Ishida Hiroyuki  東北大学, (連合)農学研究科(研究院), 准教授 (60312625)

Co-Investigator(Kenkyū-buntansha) MAKINO Amane  東北大学, 大学院農学研究科, 教授 (70181617)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2012: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Keywordsオートファジー / 窒素転流 / 栄養成長 / 生殖成長 / イネ / 葉緑体 / 老化 / 光合成 / 栄養飢餓
Outline of Final Research Achievements

We aimed to reveal roles of autophagy in the life cycle of rice and obtained following results. (1) We isolated autophagy-defective rice mutants. (2) We established monitoring methods for autophagy by generating transgenic rice expressing fluorescent protein markers. (3) We found that autophagy-defective rice mutants show delayed reproductive growth and heading and they show male sterile phenotype due to incomplete development of pollen and anther. (4) We found that autophagy-defective rice mutants show reduced biomass production and nitrogen use efficiency due to suppression of chloroplast protein degradation and nitrogen remobilisation.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (31 results)

All 2016 2015 2014 2013 2012

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (23 results) (of which Invited: 1 results)

  • [Journal Article] From Arabidopsis to cereal crops: Conservation of chloroplast protein degradation by autophagy indicates its fundamental role in plant productivity.2015

    • Author(s)
      Izumi M, Hidema J, Ishida H
    • Journal Title

      Plant Signaling & Behavior

      Volume: 10 Issue: 11 Pages: e1101199-e1101199

    • DOI

      10.1080/15592324.2015.1101199

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Establishment of monitoring methods for autophagy in rice reveals autophagic recycling of chloroplasts and root plastids during energy limitation.2015

    • Author(s)
      Izumi M, Hidema J, Wada S, Kondo E, Kurusu T, Kuchitsu K, Makino A, Ishida H.
    • Journal Title

      Plant Physiology

      Volume: 169 Issue: 4 Pages: 1307-1320

    • DOI

      10.1104/pp.114.254078

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Autophagy supports biomass production and nitrogen use efficiency at the vegetative stage in rice2015

    • Author(s)
      Wada S, Hayashida Y, Izumi M, Kurusu T, Hanamata S, Kanno K, Kojima S, Yamaya T, Kuchitsu K, Makino A, Ishida H
    • Journal Title

      Plant Physiology

      Volume: in press Issue: 1 Pages: 60-73

    • DOI

      10.1104/pp.15.00242

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Roles of autophagy in chloroplast recycling2014

    • Author(s)
      Ishida H, Izumi M, Wada S and Makino A
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1837 (4) Issue: 4 Pages: 512-521

    • DOI

      10.1016/j.bbabio.2013.11.009

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] OsATG7 is required for autophagy-dependent lipid metabolism in rice postmeiotic anther development2014

    • Author(s)
      Takamitsu Kurusu, Tomoko Koyano, Shigeru Hanamata, Takahiko Kubo, Yuhei Noguchi, Chikako Yagi, Noriko Nagata, Takashi Yamamoto, Takayuki Ohnishi, Yozo Okazaki, Nobutaka Kitahata, Daichi Ando, Masaya Ishikawa, Shinya Wada, Akio Miyao, Hirohiko Hirochika, Hiroaki Shimada, Amane Makino, Kazuki Saito, Hiroyuki Ishida, Tetsu Kjnoshita, Nori Kurata and Kazuyuki Kuchitsu^*
    • Journal Title

      Autophagy

      Volume: 10(5) Issue: 5 Pages: 878-888

    • DOI

      10.4161/auto.28279

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Deficiency of autophagy leads to significant changes of metabolic profiles in Arabidopsis.2013

    • Author(s)
      Izumi M, Hidema J, Ishida H
    • Journal Title

      Plant Signaling & Behavior

      Volume: 8 Issue: 8 Pages: 443-443

    • DOI

      10.4161/psb.25023

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evidence for contribution of autophagy to Rubisco degradation during leaf senescence in Arabidopsis thaliana.2013

    • Author(s)
      Ono Y, Wada S, Izumi M, Makino A, Ishida H
    • Journal Title

      Plant, Cell and Environment

      Volume: (In press) Issue: 6 Pages: 1147-1159

    • DOI

      10.1111/pce.12049

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Autophagy contributes to nighttime energy availability for growth in Arabidopsis.2013

    • Author(s)
      Izumi M, Hidema J, Makino A, Ishida H
    • Journal Title

      Plant Physiology

      Volume: 161 Issue: 4 Pages: 1682-1693

    • DOI

      10.1104/pp.113.215632

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 異なる窒素栄養条件下においてオートファジーの欠損がイネの窒素利用と成長に与える影響の解析2016

    • Author(s)
      横浜諒、和田慎也、菅野圭一、小島創一、山谷知行、牧野周、石田宏幸
    • Organizer
      日本植物生理学会
    • Place of Presentation
      岩手大学(岩手県盛岡市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] イネの発芽過程におけるオートファジーの役割2015

    • Author(s)
      和田慎也、中村 萌、石田宏幸、牧野 周
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      京都大学(京都府京都市左京区)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] シロイヌナズナのRCBおよび葉緑体オートファジーにおけるATI(ATG8-interacting proteins)の役割について2015

    • Author(s)
      石田宏幸、西村 翼、泉 正範、Gad Galili、牧野 周
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      京都大学(京都府京都市左京区)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] イネの栄養成長と老化葉の窒素リサイクルにおけるオートファジーの役割の解析2015

    • Author(s)
      和田慎也、林田泰和、泉 正範、来須孝光、花俣 繁、朽津和幸、牧野 周、石田宏幸
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      京都大学(京都府京都市左京区)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] シロイヌナズナにおけるオートファジーが亜鉛欠乏時に果たす役割2015

    • Author(s)
      江口雅丈、吉本光希、木村和彦、泉 正範、和田慎也、牧野 周、石田宏幸
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      京都大学(京都府京都市左京区)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] Establishment of monitoring methods for autophagy in rice reveals autophagic recycling of chloroplasts and root plastids during energy limitation2015

    • Author(s)
      Masanori Izumi,Jun Hidema,Shinya Wada,Eri Kondo,Takamitsu Kurusu,Kazuyuki Kuchitsu,Amane Makino,Hiroyuki Ishida
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東京農業大学、世田谷
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 異なる窒素栄養条件下においてオートファジーの欠損がイネの窒素利用と成長に及ぼす影響の解析2015

    • Author(s)
      横浜諒,和田慎也,牧野周,石田宏幸
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東京農業大学、世田谷
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] イネの栄養成長と老化葉の窒素リサイクルにおける オートファジーの役割の解析2015

    • Author(s)
      和田慎也,林田泰和, 泉正範,来須孝光,花俣繁,朽津和幸,牧野周,石田宏幸
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東京農業大学、世田谷
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 異なる窒素栄養条件下においてオートファジーの欠損がイネの窒素利用と成長に及ぼす影響の解析2014

    • Author(s)
      和田慎也, 横浜諒, 石田宏幸, 牧野周
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      東京農工大学、府中
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Autophagy for Photodamaged Chloroplast in Arabidopsis2014

    • Author(s)
      Izumi,M., Ishida,H., Makino,A., Hidema, J.
    • Organizer
      日本植物生理学会
    • Place of Presentation
      富山(富山大学)
    • Related Report
      2013 Annual Research Report
  • [Presentation] オートファジーの欠損がイネの栄養成長と窒素転流へ与える影響の解析2014

    • Author(s)
      林田泰和、和田慎也、来須孝光、朽津和幸、牧野周、石田宏幸
    • Organizer
      日本植物生理学会
    • Place of Presentation
      富山(富山大学)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 植物の栄養リサイクルと葉緑体のオートファジー2014

    • Author(s)
      石田宏幸
    • Organizer
      日本農芸化学会シンポジウム
    • Place of Presentation
      生田(明治大学)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] イネオートファジー欠損変異体Osatg7 の生理解析- ( その1) 栄養成長期における個体生育について-2013

    • Author(s)
      和田慎也、林田泰和、来須孝光、朽津和幸、牧野周、石田宏幸
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋(名古屋大学)
    • Related Report
      2013 Annual Research Report
  • [Presentation] イネオートファジー欠損変異体Osatg7 の生理解析-(その2)葉の老化過程における窒素転流への影響について-2013

    • Author(s)
      林田泰和、和田慎也、来須孝光、朽津和幸、牧野周、石田宏幸
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋(名古屋大学)
    • Related Report
      2013 Annual Research Report
  • [Presentation] イネにおけるRubisco-containing body/ オートファジー系の可視化2013

    • Author(s)
      泉正範、日手間純、近藤依里、牧野周、石田宏幸
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋(名古屋大学)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Role of autophagy in Rubisco degradation during leaf senescence2013

    • Author(s)
      Ishida, H., Ono, Y., Wada, S., Izumi, M., Yoshimoto, K., Mae, T., Makino, A.
    • Organizer
      6th European Workshop on Leaf Senescence
    • Place of Presentation
      フランス(INRA-Versailles)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Identification of a rice autophagy defected mutant, OsATG7, and phenotypes in the life cycle2013

    • Author(s)
      Wada, S., Hatyashida, Y., Kurusu, T., Kuchitsu, K., Makino, A., Ishida, H.
    • Organizer
      6th European Workshop on Leaf Senescence
    • Place of Presentation
      フランス(INRA-Versailles)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Autophagy in plant energy availability2013

    • Author(s)
      Izumi, M., Hirota, T., Hidema, J., Makino, A.
    • Organizer
      6th European Workshop on Leaf Senescence
    • Place of Presentation
      フランス(INRA-Versailles)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Autophagy contributes to Rubisco degradation during leaf senescence in Arabidopsis2013

    • Author(s)
      小野佑樹、和田慎也、泉正範、牧野周 石田宏幸
    • Organizer
      第54回日本植物生理学会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナのエネルギー利用におけるオートファジーの寄与について2013

    • Author(s)
      泉正範、日出間純、牧野周、石田宏幸
    • Organizer
      第54回日本植物生理学会
    • Place of Presentation
      岡山大学(岡山市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Autophagy of chloroplasts during leaf senescence2012

    • Author(s)
      Ishida H.
    • Organizer
      10th International Congress on Plant Molecular Biology
    • Place of Presentation
      済州島ICC(韓国)(招待講演)
    • Related Report
      2012 Annual Research Report
  • [Presentation] シロイヌナズナ葉の栄養素リサイクルにおいてRCB/オートファジー系が担う役割の解析2012

    • Author(s)
      泉正範、日出間純、牧野周、石田宏幸
    • Organizer
      第58回日本土壌肥料学会
    • Place of Presentation
      鳥取大学(鳥取市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Rubisco分解におけるオートファジーの貢献度の評価2012

    • Author(s)
      小野佑樹、和田慎也、泉正範、牧野周、石田宏素
    • Organizer
      第58回日本土壌肥料学会
    • Place of Presentation
      鳥取大学(鳥取市)
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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