Solution-Phase Chemical Synthesis of Insulin by using Reverse-Micellar Continuous Reaction Method
Project/Area Number |
24380061
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Bioproduction chemistry/Bioorganic chemistry
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Research Institution | Tokyo University of Agriculture and Technology |
Principal Investigator |
CHIBA Kazuhiro 東京農工大学, (連合)農学研究科(研究院), 教授 (20227325)
|
Co-Investigator(Kenkyū-buntansha) |
KIM Shokaku 東京農工大学, 大学院農学研究院, 准教授 (90537127)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
|
Keywords | ペプチド / インスリン / 電解合成 / 有機合成化学 / 化学合成 / 逆ミセル / 液相反応 |
Outline of Final Research Achievements |
Synthesis of peptide molecules of was tried by a combination of fragment synthetic technology with precise control of selective disulfide-bond formation. The reaction was performed in a reverse micellar reaction field. As a result, the new synthesis method was established for combining several peptides fragments between the aimed positions of each molecule. The peptide-chain elongation was also achieved by introducing the hydrophobic tag on each peptide fragment by using the reverse micellar reaction field. After peptide skeletons were formed, the hydrophobic tags and other deprotection reactions were performed to give key units of insulin molecu
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Report
(4 results)
Research Products
(76 results)
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[Journal Article] Tag-Assisted Liquid-Phase Peptide Synthesis Using Hydrophobic Benzyl Alcohols as Supports2013
Author(s)
Okada, Y.; Suzuki, H.; Nakae, T.; Fujita, S.; Abe, H.; Nagano, K.; Yamada, T.; Ebata, N.; Kim, S.; Chiba, K.
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Journal Title
Journal of Organic Chemistry
Volume: 78
Issue: 2
Pages: 320-327
DOI
Related Report
Peer Reviewed
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