Occurrence of progesterone in higher and lower plants and its role in sexual organ differentiation

• Project/Area Number: 24380064
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Teikyo University
• Principal Investigator: YOKOTA Takao 帝京大学, 理工学部, 教授 (40011986)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2012: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: プロゲステロン / 性分化 / トウモロコシ / 生合成 / 代謝 / ステロイド / ジャスモン酸 / オス花メス化変異体 / プロゲステロン合成酵素 / 生殖器分化 / 花芽分化

Outline of Final Research Achievements

Occurrence of progesterone was found to spread widely in the plant kingdom ranging from higher plants to lower plants including mosses and ferns. Furthermore some species of microorganisms were found to produce progesterone. Deuterated pregnenolone and progesterone were synthesized to clarify the synthesis and metabolism of progesterone. Using these deuterated steroids, it was found that pregnenolone is converted to progesterone which, in turn, is further metabolized to 5a-dihydrproesterone、allopregnanolone and epiallopregnanolone. It seems that the maize gene TS2 is involved in the synthesis of progesterone. It was also found that jasmonic acid influences the synthesis of progesterone. In addition, tobacco flowers have higher ability to convert pregnenolone to progesterone than leaves.

Research Products

• [Journal Article] A cytochrome P450, OsDSS1, is involved in growth and drought stress responses in rice (Oryza sativa L.) (2015)
  • Author(s): Tamiru, M., Undan, J. R., Takagi, H., Abe, A., Yoshida, K., Undan, J. Q., Natsume, S., Uemura, A., Saitoh, H., Matsumura, H., Urasaki, N., Yokota, T., Terauchi, R.
  • Journal Title: Plant Mol. Biol. Volume: 88 Pages: 85-99
  • DOI: 10.1007/s11103-015-0310-5
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 1. CYP90A1/CPD, a Brassinosteroid Biosynthetic Cytochrome P450 of Arabidopsis, Catalyzes C-3 Oxidation. (2012)
  • Author(s): Toshiyuki Ohnishi
  • Journal Title: J Biol Chem Volume: 287 Pages: 31551-31560
  • DOI: 10.1074/jbc.m112.392720
  • Peer Reviewed
• [Journal Article] Biosynthesis of a cholesterol-derived brassinosteroid, 28-norcastas terone, in Arabidopsis thaliana (2012)
  • Author(s): Takao Yokota(分担)
  • Journal Title: J Expt Bot Volume: 63 Pages: 1823-1833
  • DOI: 10.1093/jxb/err354
  • Peer Reviewed
• [Journal Article] How do nitrogen and phosphorus deficiencis affect strigolactone production and exudation? (2012)
  • Author(s): Kaori Yoneyama
  • Journal Title: Planta Volume: (印刷中) Pages: 1197-1207
  • DOI: 10.1007/s00425-011-1568-8
  • Peer Reviewed
• [Journal Article] Identification of marneral synthase, which is critical for growth and development in Arabidopsis (2012)
  • Author(s): Takao Yokota (分担)
  • Journal Title: Plant J Volume: 72 Pages: 791-804
  • DOI: 10.1111/j.1365-313x.2012.05120.x
  • Peer Reviewed
• [Presentation] ブラシノステロイドによるジャスモン酸の生合成の調節 (2014)
  • Author(s): 横田孝雄、湯本絵美、柴田恭美
  • Organizer: 植物化学調節学会
  • Place of Presentation: 京都大学
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] エンバクが生産する新規ストリゴラクトンの探索 (2013)
  • Author(s): 横田孝雄
  • Organizer: 農芸化学会2012年度大会(京都)
  • Place of Presentation: 京都女子大学
  • Year and Date: 2013-03-23
• [Presentation] 重水素化プロゲステロンおよび関連する重水素化誘導体の合成 (2013)
  • Author(s): 内田健一、 横田孝雄
  • Organizer: 植物化学調節学会
  • Place of Presentation: 新潟大学
• [Presentation] 高等植物におけるプロゲステロンの生合成と代謝 (2013)
  • Author(s): 湯本絵美、柴田恭美、内田健一、横田孝雄
  • Organizer: 植物化学調節学会
  • Place of Presentation: 新潟大学