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Transcriptome sequencing in cambial zone and differentiating xylem of Japanese cedar (Cryptomeria japonica) and seasonal profiling of the expressed genes throughout a growing season

Research Project

Project/Area Number 24380098
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Wood science
Research InstitutionForestry and Forest Products Research Institute

Principal Investigator

FUJIWARA Takeshi  独立行政法人森林総合研究所, 木材特性研究領域, 室長 (00353839)

Co-Investigator(Kenkyū-buntansha) WATANABE Atsushi  九州大学, 農学研究院, 准教授 (10360471)
NOSE Mine  森林総合研究所, 林木育種センター, 研究員 (20582753)
KURITA Manabu  森林総合研究所, 林木育種センター, 室長 (40370829)
IKI Taiichi  森林総合研究所, 林木育種センター, 主任研究員 (40370845)
ORIBE Yuichiro  森林総合研究所, 東北育種場, 課長 (40370853)
YAMASHITA Kana  森林総合研究所, 木材特性研究領域, 主任研究員 (60353900)
KURODA Katsushi  森林総合研究所, 木材特性研究領域, 主任研究員 (90399379)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywordsスギ / EST / マイクロアレイ / 木部形成 / 材形成 / 遺伝子発現 / データベース
Outline of Final Research Achievements

Japanese cedar is one of the most valuable species for wood industries and it has been target species of tree breeding program because of its wide diversity in wood properties. Thus, molecular aspects of wood formation offer practical information that can be used for screening and forward genetic approaches to improve wood quality.
Total RNA was isolated from samples including cambium and differentiating xylem collected from trunks of plus trees and 55,051 unique sequences were obtained. After identifying expressed sequence tags, we designed a custom cDNA microarray with 18,082 probes to compare expression of highly regulated genes throughout a growing season. Among various types of the gene expression profiles provided by the microarray, groups of genes regulated in growing season were detected, which were assigned to the functional classes such as “Carbohydrate transport and metabolism” and “Cell wall/membrane/envelope biogenesis” which are supposed to relate with wood formation.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (20 results)

All 2015 2014 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (17 results)

  • [Journal Article] 発現遺伝子による材関連遺伝子マーカー開発の可能性2014

    • Author(s)
      三嶋賢太郎、藤原健、井城泰一、黒田克志、山下香菜、田村美帆、藤澤義武、渡辺敦史
    • Journal Title

      森林遺伝育種

      Volume: 3 Pages: 167-172

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptome sequencing and profiling of expressed genes in cambial zone and differentiating xylem of Japanese cedar (Cryptomeria japonica)2014

    • Author(s)
      Kentaro Mishima, Takeshi Fujiwara, Taiichi Iki, Katsushi Kuroda, Kana Yamashita, Miho Tamura, Yoshitake Fujisawa, Atsushi Watanabe
    • Journal Title

      BMC Genomics

      Volume: 15 Issue: 1 Pages: 219-219

    • DOI

      10.1186/1471-2164-15-219

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cryo-TOF-SIMS/SEM System for analysis of the chemical distribution in freeze-fixed Cryptomeria japonica wood2012

    • Author(s)
      Katsushi Kuroda
    • Journal Title

      Surface and Interface Analysis

      Volume: (in press) Issue: 1 Pages: 215-219

    • DOI

      10.1002/sia.4979

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] スギの材質・成長形質に関するQTL解析2015

    • Author(s)
      三嶋賢太郎、平尾知士、田村美帆、井城泰一、平岡裕一郎、能勢美峰、坪村美代子、花岡創、藤原健、黒田克志、栗田学、高橋誠、渡辺敦史
    • Organizer
      第126回日本森林学会大会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2015-03-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] スギ連鎖地図作成と材質形質に関するQTLマッピング2015

    • Author(s)
      三嶋賢太郎、平尾知士、井城泰一、平岡裕一郎、坪村美代子、能勢美峰、花岡創、栗田学、高橋誠、黒田克志、藤原健、田村美帆、渡辺敦史
    • Organizer
      第65回日本木材学会大会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Year and Date
      2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 形成層活動期における材関連遺伝子の発現パターンのクローン間差2014

    • Author(s)
      三嶋賢太郎、藤原健、井城泰一、高島有哉、黒田克志、栗田学、渡辺敦史
    • Organizer
      第3回森林遺伝育種学会大会
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2014-11-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] スギ材質形質に関するQTLマッピング2014

    • Author(s)
      三嶋賢太郎、井城泰一、平尾知士、藤澤義武、藤原健、田村美帆、渡辺敦史
    • Organizer
      第64回日本木材学会大会
    • Place of Presentation
      松山
    • Related Report
      2013 Annual Research Report
  • [Presentation] 発現遺伝子解析による材関連遺伝子マーカー開発の可能性2014

    • Author(s)
      三嶋賢太郎
    • Organizer
      第125回日本森林学会大会
    • Place of Presentation
      大宮
    • Related Report
      2013 Annual Research Report
  • [Presentation] スギの温度に伴う日周性の変化2014

    • Author(s)
      能勢美峰、田村美帆、三嶋賢太郎、井城泰一、坪村美代子、栗田学、渡辺敦史
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Related Report
      2013 Annual Research Report
  • [Presentation] スギ次世代育種に向けたDNAマーカーの開発とその適用2014

    • Author(s)
      平尾知士、三嶋賢太郎、田村美帆、渡辺敦史
    • Organizer
      第125回日本森林学会大会
    • Place of Presentation
      大宮
    • Related Report
      2013 Annual Research Report
  • [Presentation] スギ次世代検定林におけるゲノムワイドマーカーを用いた形質予想2014

    • Author(s)
      内山憲太郎、岩田洋佳、木村恵、伊原徳子、上野真義、森口喜成、坪村美代子、三嶋賢太郎、井城泰一、高橋誠、渡辺敦史、二村典宏、篠原健司、津村義彦
    • Organizer
      日本育種学会第125回講演会
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] スギ器官別ESTの基盤整備2014

    • Author(s)
      田村美穂、手島康一、三嶋賢太郎、井城泰一、能勢美峰、坪村美代子、栗田学、渡辺敦史
    • Organizer
      第125回日本森林学会大会
    • Place of Presentation
      大宮
    • Related Report
      2013 Annual Research Report
  • [Presentation] スギの育種形質に対するゲノムワイド関連解析および形質予測2014

    • Author(s)
      内山憲太郎、岩田洋佳、伊原徳子、上野真義、森口喜成、坪村美代子、三嶋賢太郎、井城泰一、高橋誠、渡辺敦史、二村典宏、篠原健司、津村義彦
    • Organizer
      第125回日本森林学会大会
    • Place of Presentation
      大宮
    • Related Report
      2013 Annual Research Report
  • [Presentation] The continuity of cell division and the resumption of xylem differentiation of new cambial derivatives after the cambial reactivation induced by localizedstem heating in temperate zone trees2014

    • Author(s)
      Y. Oribe
    • Organizer
      9th International Conference on Dendrochronology
    • Place of Presentation
      メルボルン市、オーストラリア
    • Related Report
      2013 Annual Research Report
  • [Presentation] 圧縮あて材の細胞間隙形成過程に関する研究2013

    • Author(s)
      平井阿佐美
    • Organizer
      木材学会
    • Place of Presentation
      盛岡市民文化ホール(岩手県盛岡市)
    • Year and Date
      2013-03-28
    • Related Report
      2012 Annual Research Report
  • [Presentation] スギ材形成に関与する遺伝子群の探索(7) : スギ形成層帯における通年の時系列発現プロフファイル2013

    • Author(s)
      三嶋賢太郎
    • Organizer
      木材学会
    • Place of Presentation
      盛岡市民文化ホール(岩手県盛岡市)
    • Year and Date
      2013-03-28
    • Related Report
      2012 Annual Research Report
  • [Presentation] 形成層帯の休眠打破期の前後に局部加温処理を施した冷温帯に生育する常緑針葉樹(スギ)の樹幹部における木部形成2013

    • Author(s)
      織部雄一朗
    • Organizer
      木材学会
    • Place of Presentation
      盛岡市民文化ホール(岩手県盛岡市)
    • Year and Date
      2013-03-28
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒノキ、カラマツ、トドマツ心材形成過程における木部の水分布変化のCryo-SEM解析2013

    • Author(s)
      黒田克史
    • Organizer
      木材学会
    • Place of Presentation
      岩手大学(盛岡市盛岡市)
    • Year and Date
      2013-03-27
    • Related Report
      2012 Annual Research Report
  • [Presentation] スギ早期選抜にむけたEST情報の収集と網羅的発現解析2013

    • Author(s)
      三嶋賢太郎
    • Organizer
      森林学会
    • Place of Presentation
      岩手大学(盛岡市盛岡市)
    • Year and Date
      2013-03-26
    • Related Report
      2012 Annual Research Report
  • [Presentation] New Approach of Chemical Distribution in a Frozen-Hydrated Wood Sample Using the Cryo-TOF-SIMS/SEM System2012

    • Author(s)
      Katsushi Kuroda
    • Organizer
      IUFRO Division 5
    • Place of Presentation
      ポルトガル
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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