Characterization and function analysis of retinoic acid receptor (RAR) in prosobranch gastropods

• Project/Area Number: 24380110
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General fisheries
• Research Institution: National Institute for Environmental Studies
• Principal Investigator: HORIGUCHI Toshihiro 独立行政法人国立環境研究所, 環境リスク研究センター, 室長 (30260186)
• Co-Investigator(Kenkyū-buntansha): OHTA Yasuhiko 鳥取大学, 農学部, 教授 (60069078) ; MORISHITA Fumihiro 広島大学, 理学(系)研究科(研究院), 助教 (20210164)
• Co-Investigator(Renkei-kenkyūsha): IGUCHI Taisen 大学共同利用機関法人自然科学研究機構, 岡崎統合バイオサイエンスセンター, 教授 (90128588)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: 軟体動物 / 前鰓類 / レチノイン酸受容体（RAR） / レチノイドX受容体（RXR） / インポセックス / レチノイン酸受容体 (RAR) / レチノイドX受容体 (RXR) / レチノイン酸受容体（ＲＡＲ） / レチノイドＸ受容体（ＲＸＲ） / レチノイン酸受容体(RAR) / レチノイドX受容体(RXR)

Outline of Final Research Achievements

We isolated a retinoic acid receptor (RAR)-like cDNA (TcRAR) in the rock shell, Thais clavigera, and examined the transcriptional activity of the TcRAR protein by using all-trans retinoic acid (ATRA). However, no ligand-dependent transactivation was observed by this protein. We also examined the transcriptional activity of the TcRAR-ligand binding domain fused with the GAL4-DNA binding domain by using retinoic acids, retinol, and organotins and again saw no noteworthy transcriptional induction by these chemicals. Use of a mammalian two-hybrid assay to assess the interaction of the TcRAR protein with the TcRXR isoforms suggested TcRAR might form a heterodimer with the RXR isoforms. The transcriptional activity of domain-swapped TcRAR chimeric proteins (the A/B domain of TcRAR combined with the D–F domain of human RARα) was also examined and found to be ATRA-dependent. These results suggest TcRAR is not activated by retinoic acids, but can form a heterodimer with TcRXR isoforms.

Research Products

• [Journal Article] Molecular cloning of precursors for TEP-1 and TEP-2: the GGNG peptide-related peptides of a prosobranch gastropod, Thais clavigera (2015)
  • Author(s): Morishita, F., Furukawa, Y., Kodani, Y., Minakata, H., Horiguchi, T., Matsushima, O.
  • Journal Title: Peptides Volume: in press Pages: 72-82
  • DOI: 10.1016/j.peptides.2014.10.009
  • Peer Reviewed
• [Journal Article] Development of reproductive organs in the ivory shell Babylonia japonica: Observations from wild populations and laboratory-reared juveniles (2014)
  • Author(s): Horiguchi T, Ohta Y, Hamada F, Urushitani H, Cho H.S, Shiraishi H
  • Journal Title: Marine Environmental Research Volume: 93 Pages: 4-14
  • DOI: 10.1016/j.marenvres.2013.07.003
  • Peer Reviewed / Open Access
• [Journal Article] Cloning and characterization of the retinoic acid receptor-1 like protein in the rock shell, Thais clavigera. (2013)
  • Author(s): Urushitani H, Katsu Y, Ohta Y, Shiraishi H, Iguchi T, Horiguchi T
  • Journal Title: Aquat. Toxicol. Volume: 142-143 Pages: 403-413
  • DOI: 10.1016/j.aquatox.2013.09.008
  • Peer Reviewed
• [Journal Article] Specific accumulation of organotin compounds in tissues of the rock shell, Thais clavigera (2012)
  • Author(s): Horiguchi, T., Lee, J.H., Park, J.C., Cho, H.S., Shiraishi, H., Morita, M.
  • Journal Title: Marine Environmental Research Volume: VOL.76 Pages: 56-62
  • DOI: 10.1016/j.marenvres.2011.09.002
  • Peer Reviewed
• [Journal Article] Vas deferens and penis development in the imposex-exhibiting female rock shell, Thais clavigera (2012)
  • Author(s): Horiguchi, T., Ohta, Y., Urushitani, H., Lee, J.H., Park, J.C., Cho, H.S., Shiraishi, H.
  • Journal Title: Marine Environmental Research Volume: VOL.76 Pages: 71-79
  • DOI: 10.1016/j.marenvres.2011.10.001
  • Peer Reviewed
• [Presentation] Characterization and comparison of transcriptional activities of the retinoid X receptors by various organotin compounds in three prosobranch gastropods (2014)
  • Author(s): Urushitani H., Katsu Y., Kagechika H., Sousa A.C., Baarroso C.M., Ohta Y., Shiraichi H., Iguchi T., Horiguchi T.
  • Organizer: SETAC Europe 24th Annual Meeting
  • Place of Presentation: Basel (Switzerland)
  • Year and Date: 2014-05-11 – 2014-05-15
• [Presentation] 軟体動物前鰓類RXRの転写活性誘導における有機スズ化合物の影響 (2013)
  • Author(s): 漆谷博志, 勝義直, 太田康彦, 白石寛明, 井口泰泉, 堀口敏宏
  • Organizer: 環境ホルモン学会第16回研究発表会
  • Place of Presentation: 東京大学山下会館(東京都文京区)
• [Presentation] 軟体動物前鰓類RXRを用いた有機スズ化合物による転写活性誘導差異 (2012)
  • Author(s): 漆谷博志、勝義直、太田康彦、白石寛明、井口泰泉、堀口敏宏
  • Organizer: 環境ホルモン学会第15回研究発表会
  • Place of Presentation: 東京大学山上会館(東京都)
  • Year and Date: 2012-12-18
• [Presentation] Comparison of vas deferens and penis developmental between the rock shell, Tha IS clavigera(Muricidae)and the ivory shell, Babylonia japonica(Buccinidae) (2012)
  • Author(s): Horiguchi T., Ohta Y, Hamada F., Urushitani H., Cho H.S., Shiraishi H.
  • Organizer: 1st Marine NanoEcotox Workshop (5^<th> BILATERAL SEMINAR ITALY-JAPAN)
  • Place of Presentation: Consiglio Nazionale delle Ricerche, Palermo (Italy)(招待講演)
  • Year and Date: 2012-11-28
• [Presentation] Comparison of vas deferens and penis development between the rock shell, Thais clavigera (Muricidae) and the ivory shell, Babylonia japonica (Buccinidae). (2012)
  • Author(s): Horiguchi T., Ohta Y, Hamada F., Urushitani H., Cho.H.S., Shiraishi H.
  • Organizer: 6^<th> SETAC World Congress (SETAC Europe 22^<nd> Annual Meeting)
  • Place of Presentation: Estrel Berlin (Germany)
  • Year and Date: 2012-05-21