| Project/Area Number |
24380110
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | National Institute for Environmental Studies |
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Principal Investigator |
HORIGUCHI Toshihiro 独立行政法人国立環境研究所, 環境リスク研究センター, 室長 (30260186)
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| Co-Investigator(Kenkyū-buntansha) |
OHTA Yasuhiko 鳥取大学, 農学部, 教授 (60069078)
MORISHITA Fumihiro 広島大学, 理学(系)研究科(研究院), 助教 (20210164)
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| Co-Investigator(Renkei-kenkyūsha) |
IGUCHI Taisen 大学共同利用機関法人自然科学研究機構, 岡崎統合バイオサイエンスセンター, 教授 (90128588)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | 軟体動物 / 前鰓類 / レチノイン酸受容体（RAR） / レチノイドX受容体（RXR） / インポセックス / レチノイン酸受容体 (RAR) / レチノイドX受容体 (RXR) / レチノイン酸受容体（ＲＡＲ） / レチノイドＸ受容体（ＲＸＲ） / レチノイン酸受容体(RAR) / レチノイドX受容体(RXR) |
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| Outline of Final Research Achievements |
We isolated a retinoic acid receptor (RAR)-like cDNA (TcRAR) in the rock shell, Thais clavigera, and examined the transcriptional activity of the TcRAR protein by using all-trans retinoic acid (ATRA). However, no ligand-dependent transactivation was observed by this protein. We also examined the transcriptional activity of the TcRAR-ligand binding domain fused with the GAL4-DNA binding domain by using retinoic acids, retinol, and organotins and again saw no noteworthy transcriptional induction by these chemicals. Use of a mammalian two-hybrid assay to assess the interaction of the TcRAR protein with the TcRXR isoforms suggested TcRAR might form a heterodimer with the RXR isoforms. The transcriptional activity of domain-swapped TcRAR chimeric proteins (the A/B domain of TcRAR combined with the D–F domain of human RARα) was also examined and found to be ATRA-dependent. These results suggest TcRAR is not activated by retinoic acids, but can form a heterodimer with TcRXR isoforms.
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