Biosynthetic enzymes of antifouling halogenated compounds
| Project/Area Number |
24380112
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
Okino Tatsufumi 北海道大学, 地球環境科学研究科(研究院), 准教授 (30280910)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Fuyuhiko 北海道大学, 大学院地球環境科学研究院, 教授 (10219446)
UMEZAWA Taiki 北海道大学, 大学院地球環境科学研究院, 准教授 (20503618)
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| Co-Investigator(Renkei-kenkyūsha) |
NOGATA Yasuyuki 電力中央研究所, 環境科学研究所, 主任研究員 (10371535)
WASHIO Kenji 北海道大学, 大学院地球環境科学研究院, 助教 (50241302)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Keywords | 生合成 / 付着阻害 / フジツボ / 紅藻 / ブロモアレン / キプリス / ブロモペルオキシダーゼ / テルペノイド / タテジマフジツボ / マギレソゾ / ハチジョウジマレン / Omaezallene / Laurencin / ハロゲン / 付着生物 / ローレンシン / ウラソゾ |
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| Outline of Final Research Achievements |
Vanadium dependent bromoperoxidase (VBPO) was cloned from the red alga Laurencia nipponica which produces a halogenated compound, laurencin. The enzyme catalyzed cyclization and bromination of a precursor of laurencin. Bromination activity of recombinant enzyme was investigated. A similar type of VBPO was cloned from L. okamurae which produces a halogenated sesquiterpenoid, laurinterol. The recombinant enzyme showed bromination activity. In addition, another type of VBPO was cloned from L. saitoi which produces a halogenated triterpenoid. Its properties were investigated by using its recombinant protein. The structure of bromoallene containing C15 compound, omaezallene was reported. Its totaly syntehsis was successfully achieved.
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Report
(5 results)
Research Products
(21 results)
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[Journal Article] Omaezallene from red alga laurencia sp.: Structure elucidation, total synthesis, and antifouling activity2014
Author(s)
Umezawa, T., Oguri, Y., Matsuura, H., Yamazaki, S., Suzuki, M., Yoshimura, E., Furuta, T., Nogata, Y., Serisawa, Y., Matsuyama-Seisawa, K., Abe, T., Matsuda, F., Suzuki, M., Okino, T.
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Journal Title
Angewandte Chemie International Edition
Volume: 53
Issue: 15
Pages: 3909-3912
DOI
Related Report
Peer Reviewed
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[Journal Article] cDNA Cloning and Characterization of Vanadium-Dependent 3 Bromoperoxidases from the Red Alga Laurencia nipponica2014
Author(s)
Kaneko, K., Washio, K., Umezawa, T., Matsuda, F., Morikawa, M. and Okino, T.
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Journal Title
Bioscience, biotechnology, and biochemistry
Volume: 印刷中
Related Report
Peer Reviewed
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[Presentation] Vanadium dependent bromoperoxidase from the red alga Laurencia nipponica2013
Author(s)
Kaneko, K., Washio, K., Umezawa, T., Morikawa, M., Matsuda, F., and Okino, T.
Organizer
14th International Symposium on Marine Natural Products
Place of Presentation
スペイン （La Toja Island）
Related Report
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