Production of gene-designed calves using combination of highly accurate gene analysis and advanced reproductive technologies
Project/Area Number |
24380151
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Applied animal science
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Research Institution | Utsunomiya University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
FUKUI Emiko 宇都宮大学, 農学部, 准教授 (20208341)
MATSUMOTO Hiromichi 宇都宮大学, 農学部, 准教授 (70241552)
NAGAO Yosikazu 宇都宮大学, 農学部, 教授 (70291953)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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Keywords | ウシ / 遺伝子多型 / 一塩基多型 / ゲノム / 体外受精 / 繁殖 / 生殖工学 / 経済形質 / ゲノムコピー数多型 / 繁殖性 / 遺伝子 / 育種 / 育種学 / 畜産学 / 先端生殖技術 / 応用動物 / バイオテクノロジー |
Outline of Final Research Achievements |
It was aimed to produce genetically designed calves efficiently using in vitro fertilization techniques of gametes from the parents which were accurately analyzed for whole genomes. The blood samples of 110 females from the herd of the Tochigi Prefectural Livestock & Dairy Experimental Center were highly accurately analyzed for single nucleotide polymorphism (SNP) of genotypes on economically valuable genes. The genotypes of same genes were also determined for frozen semen samples purchased of ten bulls. Based on the genotyping results, combinations of ova and sperm were planned for yields of genetically superior calves. Ova were obtained from ovaries of the females of the center using OPU technics or ovaries from the slaughterhouse in Utsunomiya-city. The resulting blastocysts, which should have the desired genotypes, were obtained using the previously reported IVM-IVF-IVC system. The produced blastocysts were transferred to recipient females, but no calf was obtained until now.
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Report
(5 results)
Research Products
(12 results)
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[Journal Article] Anaslysis of a single nucleotide polymorphism in the BRCA/gene in Holstein cattle and its in mRNA expression in the gonads.2018
Author(s)
Fukui E, Zhang C, Suzuki N, Matsumoto H, Kawanobe A, Sakurai Y, Ooshima T, Muroi S, Kuwahata A, Ochi M, Yoshizawa M.
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Journal Title
J. Comp. Clin. Med.
Volume: 25
Pages: 1-8
Related Report
Peer Reviewed
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