Analysis of the genes expressed in canine lymphoma using in vitro and in vivo methodology.

• Project/Area Number: 24380170
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Yamaguchi University
• Principal Investigator: TAKUYA Mizuno 山口大学, 獣医学部, 教授 (90398826)
• Co-Investigator(Kenkyū-buntansha): TSUJIMOTO Hajime 東京大学, 大学院農学生命科学研究科, 教授 (60163804) ; SHIMIZU Kanako 統合生命医科学研究センター, 上級研究員 (20391980)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
• Keywords: リンパ腫 / 犬

Outline of Final Research Achievements

The genes specifically expressed in canine lymphoma was analyzed in vitro and in vivo. Especially, three genes were analyzed in detail, and the function was elucidated among some of them. This results would expect that these molecules could be target for lymphoma therapy in the future.

Research Products

• [Journal Article] Oncolytic effects of reovirus in canine solid tumor cell lines. (2015)
  • Author(s): Igase M, Hwang CC, Coffey M, Okuda M, Noguchi S, Mizuno T.
  • Journal Title: J Vet Med Sci. 2015 Jan16[Epub ahead of print] Volume: 印刷中
  • NAID: 130005071879
  • Peer Reviewed
• [Journal Article] Investigation of the cytotoxic effect of flavopiridol in canine lymphoma cell lines. (2015)
  • Author(s): Ema Y, Igase M, Takeda Y, Yanase T, Umeki S, Hiraoka H, Okuda M, Mizuno T.
  • Journal Title: Vet Comp Oncol. Volume: 印刷中
  • DOI: 10.1111/vco.12130
  • Peer Reviewed
• [Journal Article] The effects of oncolytic reovirus in canine lymphoma cell lines. (2015)
  • Author(s): Hwang CC, Umeki S, Igase M, Coffey M, Noguchi S, Okuda M, Mizuno T.
  • Journal Title: Vet Comp Oncol. Volume: 印刷中
  • DOI: 10.1111/vco.12124
  • Peer Reviewed
• [Journal Article] Analysis of microRNA-203 function in CREB/MITF/RAB27a pathway: comparison between canine and human melanoma cells. (2015)
  • Author(s): Noguchi S, Kumazaki M, Mori T, Baba K, Okuda M, Mizuno T, Akao Y.
  • Journal Title: Vet Comp Oncol. Volume: 印刷中
  • DOI: 10.1111/vco.12118
  • Peer Reviewed
• [Journal Article] Regulation of the development of asthmatic inflammation by in situ CD4(+)Foxp3 (+) T cells in a mouse model of late allergic asthma. (2014)
  • Author(s): Nakashima T, Hayashi T, Mizuno T.
  • Journal Title: Inflammation Volume: 37(5) Pages: 1642-1653
  • DOI: 10.1007/s10753-014-9892-3
  • Peer Reviewed
• [Journal Article] Seroepidemiology of Reovirus in Healthy Dogs in Six Prefectures in Japan. (2014)
  • Author(s): Hwang CC, Mochizuki M, Maeda K, Okuda M, Mizuno T.
  • Journal Title: J Vet Med Sci. Volume: 76 (3) Pages: 471-475
  • NAID: 130003382393
  • Peer Reviewed
• [Journal Article] Oncolytic reovirus in canine mast cell tumor. (2013)
  • Author(s): Hwang CC, Umeki S, Kubo M, Hayashi T, Shimoda H, Mochizuki M, Maeda K, Baba K, Hiraoka H, Coffey M, Okuda M, Mizuno T.
  • Journal Title: PLoS ONE Volume: 8(9)
  • Peer Reviewed
• [Journal Article] Anti-adhesive property of P-selectin glycoprotein ligand-1 (PSGL-1)due to steric hindrance effect. (2013)
  • Author(s): Umeki S, Suzuki R, Ema Y, Shimojima N, Nishinura Y, Okuda M, Mizuno T.
  • Journal Title: J Cell Biochem Volume: 114 (6) Pages: 1271-1285
  • DOI: 10.1002/jcb.24468
  • Peer Reviewed
• [Journal Article] Establishment of Five Canine Lymphoma Cell Lines and Tumor Formation in a Xenotransplantation Model (2013)
  • Author(s): Umeki, S., Ema, Y., Suzuki, R., Kubo, M., Hayashi, T., Okamura, Y., Yamazaki, J., Tsujimoto, H., Tani, K., Hiraoka, H., Okuda, M. and Mizuno, T
  • Journal Title: J. Vet. Med. Sci. Volume: 75 Issue: 4 Pages: 467-474
  • DOI: 10.1292/jvms.12-0448
  • NAID: 130002502194
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Presentation] 犬乳腺腫瘍細胞株に対する腫瘍溶解性ウイルス療法と化学療法の併用による相乗効果の検討 (2014)
  • Author(s): 伊賀瀬雅也、Hwang Chung Chew、上林聡之、下川孝子、馬場健司、奥田優、野口俊助、水野拓也
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（札幌市）
  • Year and Date: 2014-09-10
• [Presentation] イヌのPD-1およびPD-L1に対するモノクローナル抗体の作製とその機能の解析 (2014)
  • Author(s): 正司麗葉、伊賀瀬雅也、下川孝子、馬場健司、奥田優、野口俊助、水野拓也
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（札幌市）
  • Year and Date: 2014-09-10
• [Presentation] Reovirus induces poptotic cell death by the dysregulation of the Ras signling pathway (2014)
  • Author(s): Hwang Chung Chew、伊賀瀬雅也、下川孝子、馬場健司、奥田優、野口俊助、水野拓也
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（札幌市）
  • Year and Date: 2014-09-10
• [Presentation] Reovirus infection dysregulates the Ras signaling pathway in canine mast cell tumor cells (2014)
  • Author(s): Hwang CC, Coffery M, Noguchi S, Okuda M, Mizuno T.
  • Organizer: 23rd Biennial Congress of the EUROPEAN ASSOCIATION FOR CANCER RESEARCH
  • Place of Presentation: Munich（ドイツ）
  • Year and Date: 2014-07-05 – 2014-07-08
• [Presentation] 腫瘍溶解性ウイルスによる犬乳腺腫瘍細胞に対する抗腫瘍効果の検討 (2014)
  • Author(s): 伊賀瀬雅也、Chung Chew Hwang、久保正仁、中嶋朋美、林俊春、馬場健司、野口俊助、奥田優、水野拓也
  • Organizer: 日本獣医内科学アカデミー第10回記念学術大会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] 日本国内の主要犬種における犬白血球抗原I型の遺伝子解析 (2013)
  • Author(s): 中谷亮太、井上公美、川野浩志、奥田優、水野拓也
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学（岐阜市）
• [Presentation] レオウイルスによる犬の各種腫瘍細胞株に対する細胞死の誘導 (2013)
  • Author(s): 伊賀瀬雅也、Chung Chew Hwang、奥田優、水野拓也
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学（岐阜市）
• [Presentation] Reovirus effectively induces oncolysis in canine mast cell tumor in vivo and ex vivo (2013)
  • Author(s): Chung Chew Hwang、梅城沙織、伊賀瀬雅也、久保正仁、林俊春、奥田優、水野拓也
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学（岐阜市）
• [Presentation] イヌインターフェロンガンマによる犬腫瘍細胞株の増殖抑制効果の検討 (2013)
  • Author(s): 濱村友紀、奥田優、水野拓也
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学（岐阜市）
• [Presentation] イヌのリンパ腫において過剰発現する遺伝子の解析 (2012)
  • Author(s): 柳瀬拓磨、梅城沙織、酒井治、平岡博子、藤原亜紀、辻本元、奥田優、水野拓也
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学(盛岡市)
  • Year and Date: 2012-09-15
• [Presentation] イヌのTT-1に対する抗体の特異性の解析 (2012)
  • Author(s): 酒井治、中谷亮太、梅城沙織、酒井洋樹、久保正仁、森本将弘、林俊春、平岡博子、奥田優、水野拓
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学(盛岡市)
  • Year and Date: 2012-09-15
• [Presentation] 新しく樹立したイヌリンパ腫細胞株のin vitroおよびin vivoにおける性状解析 (2012)
  • Author(s): 梅城沙織、江馬康夫、鈴木綾一、久保正仁, 林俊春、岡村泰彦、山崎淳平、辻本元、谷健二、平岡博子、奥田優、水野拓也
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学(盛岡市)
  • Year and Date: 2012-09-15
• [Presentation] Discovering the oncolytic potential of reovirus in veterinary medicine : reovi rus-induced cell death in canine lymphoma and last cell tumor ceell lines (2012)
  • Author(s): Hwang Chung Chew、梅城沙織、中川貴之、下田宙、望月雅美、前田健、平岡博子、奥田優、水野拓也
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学(盛岡市)
  • Year and Date: 2012-09-15